5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils

Detalhes bibliográficos
Autor(a) principal: Luz, Ricardo Alves
Data de Publicação: 2014
Outros Autores: Xavier-Elsas, Pedro Paulo, Luca, Bianca de, Masid-de-Brito, Daniela, Cauduro, Priscila Soares, Arcanjo, Luiz Carlos Gondar, Santos, Ana Carolina Cordeiro Faria dos, Oliveira, Ivi Cristina Maria de, Gaspar-Elsas, Maria Ignez Capella
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8745
Resumo: Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Puericultura e Pediatria Martagão Gesteira. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. / Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil.
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spelling Luz, Ricardo AlvesXavier-Elsas, Pedro PauloLuca, Bianca deMasid-de-Brito, DanielaCauduro, Priscila SoaresArcanjo, Luiz Carlos GondarSantos, Ana Carolina Cordeiro Faria dosOliveira, Ivi Cristina Maria deGaspar-Elsas, Maria Ignez Capella2014-11-06T17:31:55Z2014-11-06T17:31:55Z2014LUZ, Ricardo Alves. et al. 5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils. Mediat. inflamm.,New York, 2014.https://www.arca.fiocruz.br/handle/icict/874510.1155/2014/102160engHindawi Publishing Corporation5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophilsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Puericultura e Pediatria Martagão Gesteira. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. / Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Puericultura e Pediatria Martagão Gesteira. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Puericultura e Pediatria Martagão Gesteira. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Puericultura e Pediatria Martagão Gesteira. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Puericultura e Pediatria Martagão Gesteira. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Puericultura e Pediatria Martagão Gesteira. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Puericultura e Pediatria Martagão Gesteira. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil.The roles of eosinophils in antimicrobial defense remain incompletely understood. In ovalbumin-sensitized mice, eosinophils are selectively recruited to the peritoneal cavity by antigen, eotaxin, or leukotriene(LT)B4, a 5-lipoxygenase (5-LO) metabolite. 5-LO blockade prevents responses to both antigen and eotaxin.We examined responses to eotaxin in the absence of sensitization and their dependence on 5-LO. BALB/c or PAS mice and their mutants (5-LO-deficient ALOX; eosinophil-deficient GATA-1) were injected i.p. with eotaxin, eosinophils, or both, and leukocyte accumulation was quantified up to 24 h. Significant recruitment of eosinophils by eotaxin in BALB/c, up to 24 h, was accompanied bymuch larger numbers of recruited neutrophils andmonocytes/macrophages. These effects were abolished by eotaxin neutralization and 5-LO-activating protein inhibitor MK886. In ALOX (but not PAS) mice, eotaxin recruitment was abolished for eosinophils and halved for neutrophils. In GATA-1 mutants, eotaxin recruited neither neutrophils nor macrophages. Transfer of eosinophils cultured from bone-marrow of BALB/c donors, or from ALOX donors, into GATA-1 mutant recipients, i.p., restored eotaxin recruitment of neutrophils and showed that the critical step dependent on 5-LO is the initial recruitment of eosinophils by eotaxin, not the secondary neutrophil accumulation. Eosinophil-dependent recruitment of neutrophils in naive BALB/c mice was associated with increased binding of bacteria.EosinófilosCamundongosAntígenosNeutrófilosMedula Ósseainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by....pdfapplication/pdf4182730https://www.arca.fiocruz.br/bitstream/icict/8745/1/5-Lipoxygenase-Dependent%20Recruitment%20of%20Neutrophils%20and%20Macrophages%20by....pdf4feed5498cec072c9157f73603c6f0a2MD51LICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/8745/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXT5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by....pdf.txt5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by....pdf.txtExtracted texttext/plain60697https://www.arca.fiocruz.br/bitstream/icict/8745/3/5-Lipoxygenase-Dependent%20Recruitment%20of%20Neutrophils%20and%20Macrophages%20by....pdf.txt6daf0a1f1ec26e23827a18c96eb3a73dMD53icict/87452022-08-11 11:19:45.209oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-08-11T14:19:45Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv 5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils
title 5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils
spellingShingle 5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils
Luz, Ricardo Alves
Eosinófilos
Camundongos
Antígenos
Neutrófilos
Medula Óssea
title_short 5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils
title_full 5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils
title_fullStr 5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils
title_full_unstemmed 5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils
title_sort 5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils
author Luz, Ricardo Alves
author_facet Luz, Ricardo Alves
Xavier-Elsas, Pedro Paulo
Luca, Bianca de
Masid-de-Brito, Daniela
Cauduro, Priscila Soares
Arcanjo, Luiz Carlos Gondar
Santos, Ana Carolina Cordeiro Faria dos
Oliveira, Ivi Cristina Maria de
Gaspar-Elsas, Maria Ignez Capella
author_role author
author2 Xavier-Elsas, Pedro Paulo
Luca, Bianca de
Masid-de-Brito, Daniela
Cauduro, Priscila Soares
Arcanjo, Luiz Carlos Gondar
Santos, Ana Carolina Cordeiro Faria dos
Oliveira, Ivi Cristina Maria de
Gaspar-Elsas, Maria Ignez Capella
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Luz, Ricardo Alves
Xavier-Elsas, Pedro Paulo
Luca, Bianca de
Masid-de-Brito, Daniela
Cauduro, Priscila Soares
Arcanjo, Luiz Carlos Gondar
Santos, Ana Carolina Cordeiro Faria dos
Oliveira, Ivi Cristina Maria de
Gaspar-Elsas, Maria Ignez Capella
dc.subject.decs.pt_BR.fl_str_mv Eosinófilos
Camundongos
Antígenos
Neutrófilos
Medula Óssea
topic Eosinófilos
Camundongos
Antígenos
Neutrófilos
Medula Óssea
description Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Puericultura e Pediatria Martagão Gesteira. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. / Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-11-06T17:31:55Z
dc.date.available.fl_str_mv 2014-11-06T17:31:55Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LUZ, Ricardo Alves. et al. 5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils. Mediat. inflamm.,New York, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8745
dc.identifier.doi.pt_BR.fl_str_mv 10.1155/2014/102160
identifier_str_mv LUZ, Ricardo Alves. et al. 5-Lipoxygenase-dependent recruitment of neutrophils and macrophages by eotaxin-stimulated murine eosinophils. Mediat. inflamm.,New York, 2014.
10.1155/2014/102160
url https://www.arca.fiocruz.br/handle/icict/8745
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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