Arsenic trioxide triggers apoptosis of metastatic oral squamous cells carcinoma with concomitant downregulation of gli1 in hedgehog signaling
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/57106 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). INOVA FIOCRUZ. Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). |
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Nogueira, Raphael Luís RochaAraújo, Taís Bacelar Sacramento deValverde, Ludmila FaroSilva, Viviane Aline OliveiraCavalcante, Bruno Raphael RibeiroRossi, Erik AranhaAllahdadi, Kyan JamesReis, Mitermayer Galvão dosPereira, Thiago AlmeidaColetta, Ricardo D.Bezerra, Daniel PereiraSouza, Bruno Solano de FreitasDias, Rosane BorgesRocha, Clarissa A. Gurgel2023-02-24T12:24:53Z2023-02-24T12:24:53Z2022NOGUEIRA, Raphael Luís Rocha et al. Arsenic Trioxide Triggers Apoptosis of Metastatic Oral Squamous Cells Carcinoma with Concomitant Downregulation of GLI1 in Hedgehog Signaling. Biomedicines, v. 10, p. 1-13, 2022.2227-9059https://www.arca.fiocruz.br/handle/icict/5710610.3390/biomedicines10123293Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). INOVA FIOCRUZ. Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Patologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Odontologia. Departamento de Propedêutica. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Odontologia. Departamento de Propedêutica. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Patologia. Salvador, BA, Brasil / Hospital do Câncer de Barretos. Centro de Pesquisas em Oncologia Molecular. Barretos, SP, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Patologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Patologia. Salvador, BA, Brasil / Hospital São Rafael. Instituto D’Or de Pesquisa e Ensino. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil.Hospital São Rafael. Instituto D’Or de Pesquisa e Ensino. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Patologia. Salvador, BA, Brasil.Institute for Stem Cell Biology and Regenerative Medicine. Stanford University. Stanford, CA, USA.Universidade Estadual de Campinas. Faculdade de Odontologia. Departamento de Diagnóstico Bucal. Piracicaba, SP, Brasil / Universidade Estadual de Campinas. Faculdade de Odontologia. Programa de Pós-Graduação em Biologia Oral. Piracicaba, SP, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Patologia. Salvador, BA, Brasil/ Hospital São Rafael. Instituto D’Or de Pesquisa e Ensino. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Odontologia. Departamento de Propedêutica. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Departamento de Patologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Odontologia. Departamento de Propedêutica. Salvador, BA, Brasil / Hospital São Rafael. Instituto D’Or de Pesquisa e Ensino. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil.Given the lack of advances in Oral Squamous Cell Carcinoma (OSCC) therapy in recent years, pharmacological strategies to block OSCC-related signaling pathways have gained prominence. The present study aimed to evaluate the therapeutic potential of Arsenic Trioxide (ATO) concerning its antitumoral effects and the inhibition of the Hedgehog (HH) pathway in OSCC. Initially, ATO cytotoxicity was assessed in a panel of cell lines. Cell viability, cell cycle, death patterns, and cell morphology were analyzed, as well as the effect of ATO on the expression of HH pathway components. After the cytotoxic assay, HSC3 cells were chosen for all in vitro assays. ATO increased apoptotic cell death and nuclear fragmentation in the sub-G1 cell cycle phase and promoted changes in cell morphology. In addition, the reduced expression of GLI1 indicated that ATO inhibits HH activity. The present study provides evidence of ATO as an effective cytotoxic drug for oral cancer treatment in vitro.engMDPICâncer bucalSinalização ouriçoTrióxido de arsênicoGLI1Reaproveitamento de medicamentosOral cancerHedgehog signalingArsenic trioxideGLI1Drug repurposingNeoplasias bucaisTrióxido de arsênioArsenic trioxide triggers apoptosis of metastatic oral squamous cells carcinoma with concomitant downregulation of gli1 in hedgehog signalinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57106/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALNogueira,R.L.R. Arsenic trioxide triggers...pdfNogueira,R.L.R. Arsenic trioxide triggers...pdfapplication/pdf4128865https://www.arca.fiocruz.br/bitstream/icict/57106/2/Nogueira%2cR.L.R.%20Arsenic%20trioxide%20triggers...pdfa53266b5bd2606064a02384ada027425MD52icict/571062023-03-15 14:32:52.583oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:52Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Arsenic trioxide triggers apoptosis of metastatic oral squamous cells carcinoma with concomitant downregulation of gli1 in hedgehog signaling |
| title |
Arsenic trioxide triggers apoptosis of metastatic oral squamous cells carcinoma with concomitant downregulation of gli1 in hedgehog signaling |
| spellingShingle |
Arsenic trioxide triggers apoptosis of metastatic oral squamous cells carcinoma with concomitant downregulation of gli1 in hedgehog signaling Nogueira, Raphael Luís Rocha Câncer bucal Sinalização ouriço Trióxido de arsênico GLI1 Reaproveitamento de medicamentos Oral cancer Hedgehog signaling Arsenic trioxide GLI1 Drug repurposing Neoplasias bucais Trióxido de arsênio |
| title_short |
Arsenic trioxide triggers apoptosis of metastatic oral squamous cells carcinoma with concomitant downregulation of gli1 in hedgehog signaling |
| title_full |
Arsenic trioxide triggers apoptosis of metastatic oral squamous cells carcinoma with concomitant downregulation of gli1 in hedgehog signaling |
| title_fullStr |
Arsenic trioxide triggers apoptosis of metastatic oral squamous cells carcinoma with concomitant downregulation of gli1 in hedgehog signaling |
| title_full_unstemmed |
Arsenic trioxide triggers apoptosis of metastatic oral squamous cells carcinoma with concomitant downregulation of gli1 in hedgehog signaling |
| title_sort |
Arsenic trioxide triggers apoptosis of metastatic oral squamous cells carcinoma with concomitant downregulation of gli1 in hedgehog signaling |
| author |
Nogueira, Raphael Luís Rocha |
| author_facet |
Nogueira, Raphael Luís Rocha Araújo, Taís Bacelar Sacramento de Valverde, Ludmila Faro Silva, Viviane Aline Oliveira Cavalcante, Bruno Raphael Ribeiro Rossi, Erik Aranha Allahdadi, Kyan James Reis, Mitermayer Galvão dos Pereira, Thiago Almeida Coletta, Ricardo D. Bezerra, Daniel Pereira Souza, Bruno Solano de Freitas Dias, Rosane Borges Rocha, Clarissa A. Gurgel |
| author_role |
author |
| author2 |
Araújo, Taís Bacelar Sacramento de Valverde, Ludmila Faro Silva, Viviane Aline Oliveira Cavalcante, Bruno Raphael Ribeiro Rossi, Erik Aranha Allahdadi, Kyan James Reis, Mitermayer Galvão dos Pereira, Thiago Almeida Coletta, Ricardo D. Bezerra, Daniel Pereira Souza, Bruno Solano de Freitas Dias, Rosane Borges Rocha, Clarissa A. Gurgel |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nogueira, Raphael Luís Rocha Araújo, Taís Bacelar Sacramento de Valverde, Ludmila Faro Silva, Viviane Aline Oliveira Cavalcante, Bruno Raphael Ribeiro Rossi, Erik Aranha Allahdadi, Kyan James Reis, Mitermayer Galvão dos Pereira, Thiago Almeida Coletta, Ricardo D. Bezerra, Daniel Pereira Souza, Bruno Solano de Freitas Dias, Rosane Borges Rocha, Clarissa A. Gurgel |
| dc.subject.other.en_US.fl_str_mv |
Câncer bucal Sinalização ouriço Trióxido de arsênico GLI1 Reaproveitamento de medicamentos |
| topic |
Câncer bucal Sinalização ouriço Trióxido de arsênico GLI1 Reaproveitamento de medicamentos Oral cancer Hedgehog signaling Arsenic trioxide GLI1 Drug repurposing Neoplasias bucais Trióxido de arsênio |
| dc.subject.en.en_US.fl_str_mv |
Oral cancer Hedgehog signaling Arsenic trioxide GLI1 Drug repurposing |
| dc.subject.decs.en_US.fl_str_mv |
Neoplasias bucais Trióxido de arsênio |
| description |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). INOVA FIOCRUZ. Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). |
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2022 |
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2022 |
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2023-02-24T12:24:53Z |
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2023-02-24T12:24:53Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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NOGUEIRA, Raphael Luís Rocha et al. Arsenic Trioxide Triggers Apoptosis of Metastatic Oral Squamous Cells Carcinoma with Concomitant Downregulation of GLI1 in Hedgehog Signaling. Biomedicines, v. 10, p. 1-13, 2022. |
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https://www.arca.fiocruz.br/handle/icict/57106 |
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2227-9059 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/biomedicines10123293 |
| identifier_str_mv |
NOGUEIRA, Raphael Luís Rocha et al. Arsenic Trioxide Triggers Apoptosis of Metastatic Oral Squamous Cells Carcinoma with Concomitant Downregulation of GLI1 in Hedgehog Signaling. Biomedicines, v. 10, p. 1-13, 2022. 2227-9059 10.3390/biomedicines10123293 |
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MDPI |
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