Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments

Detalhes bibliográficos
Autor(a) principal: Horodesky, Aline
Data de Publicação: 2020
Outros Autores: Castilho-Westphal, Gisela G., Pont, Giorgi Dal, Faoro, Helisson, Balsanelli, Eduardo, Tadra-Sfeir, Michelle Z., Cozer, Nathieli, Pie, Marcio Roberto, Ostrensky, Antonio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42394
Resumo: Universidade Federal do Paraná. Setor de Ciências Agrárias. Grupo Integrado de Agricultura e Estudos Ambientais. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Setor de Ciências Biológicas. Programa de Pós-Graduação em Zoologia, Curitiba, PR, Brasil.
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spelling Horodesky, AlineCastilho-Westphal, Gisela G.Pont, Giorgi DalFaoro, HelissonBalsanelli, EduardoTadra-Sfeir, Michelle Z.Cozer, NathieliPie, Marcio RobertoOstrensky, Antonio2020-07-24T22:07:12Z2020-07-24T22:07:12Z2020HORODESKY, Aline et al. Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments. Anais da Academia Brasileira de Ciências, v.92, supl.1, p. 1-15, 2020.1678-2690https://www.arca.fiocruz.br/handle/icict/4239410.1590/0001-37652020201804321678-2690porAcademia Brasileira de CiênciasOysterOstraBiological ContaminationOstreidaeVirulencePathogenicityContaminación BiológicaVirulenciaContaminação BiológicaVirulênciaMetagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environmentsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Setor de Ciências Agrárias. Grupo Integrado de Agricultura e Estudos Ambientais. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Setor de Ciências Biológicas. Programa de Pós-Graduação em Zoologia, Curitiba, PR, Brasil.Universidade Federal do Paraná. Setor de Ciências Agrárias. Grupo Integrado de Agricultura e Estudos Ambientais. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Setor de Ciências Biológicas. Programa de Pós-Graduação em Zoologia. Curitiba, PR, Brasil.Universidade Federal do Paraná. Setor de Ciências Agrárias. Grupo Integrado de Agricultura e Estudos Ambientais. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Setor de Ciências Biológicas. Programa de Pós-Graduação em Zoologia. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Setor de Ciências Agrárias. Programa de Pós-Graduação em Zootecnia, Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal do Paraná. Setor de Ciências Biológicas. Departamento de Bioquímica. Curitiba, PR, Brasil.Universidade Federal do Paraná. Setor de Ciências Biológicas. Departamento de Bioquímica. Curitiba, PR, Brasil.Universidade Federal do Paraná. Setor de Ciências Agrárias. Grupo Integrado de Agricultura e Estudos Ambientais. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Setor de Ciências Agrárias. Programa de Pós-Graduação em Zootecnia. Curitiba, PR, Brasil.Universidade Federal do Paraná. Setor de Ciências Biológicas. Programa de Pós-Graduação em Zoologia Curitiba. PR, Brasil.Universidade Federal do Paraná. Setor de Ciências Agrárias. Grupo Integrado de Agricultura e Estudos Ambientais. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Setor de Ciências Biológicas. Programa de Pós-Graduação em Zoologia, Curitiba, PR, Brasil. / Universidade Federal do Paraná. Setor de Ciências Agrárias. Programa de Pós-Graduação em Zootecnia, Curitiba, PR, Brasil.In this work, we identified the bacterial microbiota associated with farmed oystersin estuarine regions of four states in the north eastern region of Brazil. During the drought and rainy seasons, for eight months, twenty oysters were sampled seasonally from seven different marine farms. In the laboratory, DNA extraction, amplification, and sequencing of the 16S rRNA gene were performed to establish the taxonomic units. We identified 106 genera of bacteria belonging to 103 families, 70 orders, 39 classes, and 21 phyla. Out of the total, 40 of the genera represented bacteria potentially pathogenic to humans; of these, nine are known to cause foodborne diseases and six are potentially pathogenic to oysters. The most prevalent genera were Mycoplasma, Propionigenium, Psychrilyobacter, and Arcobacter. The results indicate the need for more systematic monitoring of bacteria of the genus Mycoplasma in oyster farming operations in the Brazilian north eastern region. Currently, Mycoplasma is not one of the microorganisms analysed and monitored by order of Brazilian legislation during the oyster production and/or commercialization process, even though this genus was the most prevalent at all sampling points and presents pathogenic potential both for oysters and for consumers.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL0001-3765-aabc-92.pdf0001-3765-aabc-92.pdfapplication/pdf436895https://www.arca.fiocruz.br/bitstream/icict/42394/2/0001-3765-aabc-92.pdfa21e92ab8161c6174750a0638a1b43e6MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42394/3/license.txt5a560609d32a3863062d77ff32785d58MD53TEXT0001-3765-aabc-92.pdf.txt0001-3765-aabc-92.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments
title Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments
spellingShingle Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments
Horodesky, Aline
Oyster
Ostra
Biological Contamination
Ostreidae
Virulence
Pathogenicity
Contaminación Biológica
Virulencia
Contaminação Biológica
Virulência
title_short Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments
title_full Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments
title_fullStr Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments
title_full_unstemmed Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments
title_sort Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments
author Horodesky, Aline
author_facet Horodesky, Aline
Castilho-Westphal, Gisela G.
Pont, Giorgi Dal
Faoro, Helisson
Balsanelli, Eduardo
Tadra-Sfeir, Michelle Z.
Cozer, Nathieli
Pie, Marcio Roberto
Ostrensky, Antonio
author_role author
author2 Castilho-Westphal, Gisela G.
Pont, Giorgi Dal
Faoro, Helisson
Balsanelli, Eduardo
Tadra-Sfeir, Michelle Z.
Cozer, Nathieli
Pie, Marcio Roberto
Ostrensky, Antonio
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Horodesky, Aline
Castilho-Westphal, Gisela G.
Pont, Giorgi Dal
Faoro, Helisson
Balsanelli, Eduardo
Tadra-Sfeir, Michelle Z.
Cozer, Nathieli
Pie, Marcio Roberto
Ostrensky, Antonio
dc.subject.other.pt_BR.fl_str_mv Oyster
Ostra
topic Oyster
Ostra
Biological Contamination
Ostreidae
Virulence
Pathogenicity
Contaminación Biológica
Virulencia
Contaminação Biológica
Virulência
dc.subject.en.pt_BR.fl_str_mv Biological Contamination
Ostreidae
Virulence
Pathogenicity
dc.subject.es.pt_BR.fl_str_mv Contaminación Biológica
Virulencia
dc.subject.decs.pt_BR.fl_str_mv Contaminação Biológica
Virulência
description Universidade Federal do Paraná. Setor de Ciências Agrárias. Grupo Integrado de Agricultura e Estudos Ambientais. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Setor de Ciências Biológicas. Programa de Pós-Graduação em Zoologia, Curitiba, PR, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-07-24T22:07:12Z
dc.date.available.fl_str_mv 2020-07-24T22:07:12Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HORODESKY, Aline et al. Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments. Anais da Academia Brasileira de Ciências, v.92, supl.1, p. 1-15, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42394
dc.identifier.issn.pt_BR.fl_str_mv 1678-2690
dc.identifier.doi.none.fl_str_mv 10.1590/0001-3765202020180432
dc.identifier.eissn.none.fl_str_mv 1678-2690
identifier_str_mv HORODESKY, Aline et al. Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments. Anais da Academia Brasileira de Ciências, v.92, supl.1, p. 1-15, 2020.
1678-2690
10.1590/0001-3765202020180432
url https://www.arca.fiocruz.br/handle/icict/42394
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/42394/4/0001-3765-aabc-92.pdf.txt
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