Trypanosoma cruzi Presenilin-like transmembrane aspartyl protease: charactherization and cellular localization
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44850 |
Resumo: | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciada. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. |
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Lechuga, Guilherme CurtyPêgo, Paloma NapoleãoBottino, Carolina G.Pinho, Rosa T.Provance Junior, David WilliamDe Simone, Salvatore Giovanni2020-12-11T18:10:32Z2020-12-11T18:10:32Z2020LECHUGA, Guilherme C. et al. Trypanosoma cruzi Presenilin-Like Transmembrane Aspartyl Protease: Characterization and Cellular Localization, Biomolecules, v. 10, n. 11, p. 1-20, Nov, 2020.2218-273Xhttps://www.arca.fiocruz.br/handle/icict/4485010.3390/biom10111564engMDPITrypanosoma cruziPresenilinaAnticorpos anti-peptídeoSíntese SPOTImunolocalizaçãoDomínios transmembranaTrypanosoma cruzi; presenilinAspartic proteaseAnti-peptide antibodiesSPOT-synthesisImmunolocalizationTransmembrane domainsTrypanosoma cruzi Presenilin-like transmembrane aspartyl protease: charactherization and cellular localizationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciada. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciada. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisa Médica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciada. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.The increasing detection of infections of Trypanosoma cruzi, the etiological agent of Chagas disease, in non-endemic regions beyond Latin America has risen to be a major public health issue. With an impact in the millions of people, current treatments rely on antiquated drugs that produce severe side effects and are considered nearly ineffective for the chronic phase. The minimal progress in the development of new drugs highlights the need for advances in basic research on crucial biochemical pathways in T. cruzi to identify new targets. Here, we report on the T. cruzi presenilin-like transmembrane aspartyl enzyme, a protease of the aspartic class in a unique phylogenetic subgroup with T. vivax separate from protozoans. Computational analyses suggest it contains nine transmembrane domains and an active site with the characteristic PALP motif of the A22 family. Multiple linear B-cell epitopes were identified by SPOT-synthesis analysis with Chagasic patient sera. Two were chosen to generate rabbit antisera, whose signal was primarily localized to the flagellar pocket, intracellular vesicles, and endoplasmic reticulum in parasites by whole-cell immunofluorescence. The results suggest that the parasitic presenilin-like enzyme could have a role in the secretory pathway and serve as a target for the generation of new therapeutics specific to the T. cruzi.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44850/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSlavatoredeSimone_RosaPinho_etal_IOC-2020.pdfSlavatoredeSimone_RosaPinho_etal_IOC-2020.pdfapplication/pdf2063899https://www.arca.fiocruz.br/bitstream/icict/44850/2/SlavatoredeSimone_RosaPinho_etal_IOC-2020.pdf2e68526bad01eaa717c9fa2861fedd17MD52TEXTSlavatoredeSimone_RosaPinho_etal_IOC-2020.pdf.txtSlavatoredeSimone_RosaPinho_etal_IOC-2020.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Trypanosoma cruzi Presenilin-like transmembrane aspartyl protease: charactherization and cellular localization |
| title |
Trypanosoma cruzi Presenilin-like transmembrane aspartyl protease: charactherization and cellular localization |
| spellingShingle |
Trypanosoma cruzi Presenilin-like transmembrane aspartyl protease: charactherization and cellular localization Lechuga, Guilherme Curty Trypanosoma cruzi Presenilina Anticorpos anti-peptídeo Síntese SPOT Imunolocalização Domínios transmembrana Trypanosoma cruzi; presenilin Aspartic protease Anti-peptide antibodies SPOT-synthesis Immunolocalization Transmembrane domains |
| title_short |
Trypanosoma cruzi Presenilin-like transmembrane aspartyl protease: charactherization and cellular localization |
| title_full |
Trypanosoma cruzi Presenilin-like transmembrane aspartyl protease: charactherization and cellular localization |
| title_fullStr |
Trypanosoma cruzi Presenilin-like transmembrane aspartyl protease: charactherization and cellular localization |
| title_full_unstemmed |
Trypanosoma cruzi Presenilin-like transmembrane aspartyl protease: charactherization and cellular localization |
| title_sort |
Trypanosoma cruzi Presenilin-like transmembrane aspartyl protease: charactherization and cellular localization |
| author |
Lechuga, Guilherme Curty |
| author_facet |
Lechuga, Guilherme Curty Pêgo, Paloma Napoleão Bottino, Carolina G. Pinho, Rosa T. Provance Junior, David William De Simone, Salvatore Giovanni |
| author_role |
author |
| author2 |
Pêgo, Paloma Napoleão Bottino, Carolina G. Pinho, Rosa T. Provance Junior, David William De Simone, Salvatore Giovanni |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Lechuga, Guilherme Curty Pêgo, Paloma Napoleão Bottino, Carolina G. Pinho, Rosa T. Provance Junior, David William De Simone, Salvatore Giovanni |
| dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi Presenilina Anticorpos anti-peptídeo Síntese SPOT Imunolocalização Domínios transmembrana |
| topic |
Trypanosoma cruzi Presenilina Anticorpos anti-peptídeo Síntese SPOT Imunolocalização Domínios transmembrana Trypanosoma cruzi; presenilin Aspartic protease Anti-peptide antibodies SPOT-synthesis Immunolocalization Transmembrane domains |
| dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi; presenilin Aspartic protease Anti-peptide antibodies SPOT-synthesis Immunolocalization Transmembrane domains |
| description |
Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciada. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. |
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2020 |
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2020-12-11T18:10:32Z |
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2020-12-11T18:10:32Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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LECHUGA, Guilherme C. et al. Trypanosoma cruzi Presenilin-Like Transmembrane Aspartyl Protease: Characterization and Cellular Localization, Biomolecules, v. 10, n. 11, p. 1-20, Nov, 2020. |
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https://www.arca.fiocruz.br/handle/icict/44850 |
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2218-273X |
| dc.identifier.doi.none.fl_str_mv |
10.3390/biom10111564 |
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LECHUGA, Guilherme C. et al. Trypanosoma cruzi Presenilin-Like Transmembrane Aspartyl Protease: Characterization and Cellular Localization, Biomolecules, v. 10, n. 11, p. 1-20, Nov, 2020. 2218-273X 10.3390/biom10111564 |
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MDPI |
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MDPI |
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