PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic

Detalhes bibliográficos
Autor(a) principal: Martinez, Alejandra Nóbrega
Data de Publicação: 2014
Outros Autores: Talhari, Carolina, Moraes, Milton Ozório, Talhari, Sinésio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10478
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil / Tulane National Primate Research Center. Divisions of Bacteriology and Parasitology. Louisiania, USA.
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spelling Martinez, Alejandra NóbregaTalhari, CarolinaMoraes, Milton OzórioTalhari, Sinésio2015-05-22T18:42:45Z2015-05-22T18:42:45Z2014MARTINEZ, Alejandra Nóbrega et al. PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic. PLOS Neglected Tropical Diseases, v.8, n.4, 8p, 2014.https://www.arca.fiocruz.br/handle/icict/1047810.1371/journal.pntd.0002655engPlos OneHanseníaseLeprosyMycobacterium lepraePCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinicinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil / Tulane National Primate Research Center. Divisions of Bacteriology and Parasitology. Louisiania, USA.Fundação Alfredo da Matta. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Universidade Nilton Lins. Manaus, AM, Brasil.In leprosy, classic diagnostic tools based on bacillary counts and histopathology have been facing hurdles, especially in distinguishing latent infection from active disease and diagnosing paucibacillary clinical forms. Serological tests and IFN-gamma releasing assays (IGRA) that employ humoral and cellular immune parameters, respectively, are also being used, but recent results indicate that quantitative PCR (qPCR) is a key technique due to its higher sensitivity and specificity. In fact, advances concerning the structure and function of the Mycobacterium leprae genome led to the development of specific PCR-based gene amplification assays for leprosy diagnosis and monitoring of household contacts. Also, based on the validation of point-of-care technologies for M. tuberculosis DNA detection, it is clear that the same advantages of rapid DNA detection could be observed in respect to leprosy. So far, PCR has proven useful in the determination of transmission routes, M. leprae viability, and drug resistance in leprosy. However, PCR has been ascertained to be especially valuable in diagnosing difficult cases like pure neural leprosy (PNL), paucibacillary (PB), and patients with atypical clinical presentation and histopathological features compatible with leprosy. Also, the detection of M. leprae DNA in different samples of the household contacts of leprosy patients is very promising. Although a positive PCR result is not sufficient to establish a causal relationship with disease outcome, quantitation provided by qPCR is clearly capable of indicating increased risk of developing the disease and could alert clinicians to follow these contacts more closely or even define rules for chemoprophylaxis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10478/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALalejandra_martinezetal_IOC_2014.pdfapplication/pdf211978https://www.arca.fiocruz.br/bitstream/icict/10478/2/alejandra_martinezetal_IOC_2014.pdf016e6ec5b676ea1547991d9506ce858fMD52TEXTalejandra_martinezetal_IOC_2014.pdf.txtalejandra_martinezetal_IOC_2014.pdf.txtExtracted texttext/plain57320https://www.arca.fiocruz.br/bitstream/icict/10478/3/alejandra_martinezetal_IOC_2014.pdf.txt16e766279ee5fb72422bbc25b9fb6933MD53icict/104782022-06-24 12:19:40.289oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:19:40Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic
title PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic
spellingShingle PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic
Martinez, Alejandra Nóbrega
Hanseníase
Leprosy
Mycobacterium leprae
title_short PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic
title_full PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic
title_fullStr PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic
title_full_unstemmed PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic
title_sort PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic
author Martinez, Alejandra Nóbrega
author_facet Martinez, Alejandra Nóbrega
Talhari, Carolina
Moraes, Milton Ozório
Talhari, Sinésio
author_role author
author2 Talhari, Carolina
Moraes, Milton Ozório
Talhari, Sinésio
author2_role author
author
author
dc.contributor.author.fl_str_mv Martinez, Alejandra Nóbrega
Talhari, Carolina
Moraes, Milton Ozório
Talhari, Sinésio
dc.subject.other.pt_BR.fl_str_mv Hanseníase
topic Hanseníase
Leprosy
Mycobacterium leprae
dc.subject.en.pt_BR.fl_str_mv Leprosy
Mycobacterium leprae
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil / Tulane National Primate Research Center. Divisions of Bacteriology and Parasitology. Louisiania, USA.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-22T18:42:45Z
dc.date.available.fl_str_mv 2015-05-22T18:42:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARTINEZ, Alejandra Nóbrega et al. PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic. PLOS Neglected Tropical Diseases, v.8, n.4, 8p, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10478
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pntd.0002655
identifier_str_mv MARTINEZ, Alejandra Nóbrega et al. PCR-Based Techniques for Leprosy Diagnosis: From the Laboratory to the Clinic. PLOS Neglected Tropical Diseases, v.8, n.4, 8p, 2014.
10.1371/journal.pntd.0002655
url https://www.arca.fiocruz.br/handle/icict/10478
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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