TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosomacruzi

Detalhes bibliográficos
Autor(a) principal: Bombaça, Ana Cristina Souza
Data de Publicação: 2018
Outros Autores: Dossow, Daniela Von, Barbosa, Juliana Magalhães Chaves, Paz, Cristian, Burgos, Viviana, Menna-Barreto, Rubem F. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31505
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
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spelling Bombaça, Ana Cristina SouzaDossow, Daniela VonBarbosa, Juliana Magalhães ChavesPaz, CristianBurgos, VivianaMenna-Barreto, Rubem F. S.2019-02-08T18:01:54Z2019-02-08T18:01:54Z2018BOMBAÇA, Ana Cristina et al. TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosoma cruzi. Molecules, v. 23, n. 2800, p. 1-14, 2018.1420-3049https://www.arca.fiocruz.br/handle/icict/3150510.3390/molecules231128001420-3049engMDPITrypanosomacruziDoença de ChagasQuimioterapiaAutofagiaMitocôndriaSesquiterpenóidesTrypanosomacruziChagas DiseaseChemotherapySesquiterpenoidsMitochondriaAutophagyTrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosomacruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Universidad de la Frontera. Departamento de Ciencias Químicas y Recursos Naturales. Temuco, Chile.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Universidad de la Frontera. Departamento de Ciencias Químicas y Recursos Naturales. Temuco, Chile.Universidad de la Frontera. Departamento de Ciencias Químicas y Recursos Naturales. Temuco, Chile.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Chagas disease is a neglected tropical disease that is caused by the protozoan Trypanosomacruzi and represents a serious health problem, especially in Latin America. The clinical treatment of Chagas disease is based on two nitroderivatives that present severe side effects and important limitations. In folk medicine, natural products, including sesquiterpenoids, have been employed for the treatment of different parasitic diseases. In this study, the trypanocidal activity of compounds isolated from the Chilean plants Drimyswinteri, Podanthusmitiquiand Maytenusboaria on three T.cruzi evolutive forms (epimastigote, trypomastigote and amastigote) was evaluated. Total extracts and seven isolated sesquiterpenoids were assayed on trypomastigotes and epimastigotes. Polygodial (Pgd) from D.winteri, total extract from P.mitiqui (PmTE) and the germacrane erioflorin (Efr) from P.mitiqui were the most bioactive substances. Pgd, Efr and PmTE also presented strong effects on intracellular amastigotes and low host toxicity. Many ultrastructural effects of these substances, including reservosome disruption, cytosolic vacuolization, autophagic phenotype and mitochondrial swelling (in the case of Pgd), were observed. Flow cytometric analysis demonstrated a reduction in mitochondrial membrane potential in treated epimastigotes and an increase in ROS production and high plasma membrane permeability after treatment with Pgd. The promising trypanocidal activity of these natural sesquiterpenoids may be a good starting point for the development of alternative treatments for Chagas disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31505/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjulianaMC_barbosa_etal_IOC_2018.pdfjulianaMC_barbosa_etal_IOC_2018.pdfapplication/pdf3576015https://www.arca.fiocruz.br/bitstream/icict/31505/2/julianaMC_barbosa_etal_IOC_2018.pdff63f6167ff7a0ff18c4cd59fa93e0805MD52TEXTjulianaMC_barbosa_etal_IOC_2018.pdf.txtjulianaMC_barbosa_etal_IOC_2018.pdf.txtExtracted texttext/plain64426https://www.arca.fiocruz.br/bitstream/icict/31505/3/julianaMC_barbosa_etal_IOC_2018.pdf.txtbffeadaffcc672bae4e7c28a39b54598MD53icict/315052019-02-10 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dc.title.pt_BR.fl_str_mv TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosomacruzi
title TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosomacruzi
spellingShingle TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosomacruzi
Bombaça, Ana Cristina Souza
Trypanosomacruzi
Doença de Chagas
Quimioterapia
Autofagia
Mitocôndria
Sesquiterpenóides
Trypanosomacruzi
Chagas Disease
Chemotherapy
Sesquiterpenoids
Mitochondria
Autophagy
title_short TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosomacruzi
title_full TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosomacruzi
title_fullStr TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosomacruzi
title_full_unstemmed TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosomacruzi
title_sort TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosomacruzi
author Bombaça, Ana Cristina Souza
author_facet Bombaça, Ana Cristina Souza
Dossow, Daniela Von
Barbosa, Juliana Magalhães Chaves
Paz, Cristian
Burgos, Viviana
Menna-Barreto, Rubem F. S.
author_role author
author2 Dossow, Daniela Von
Barbosa, Juliana Magalhães Chaves
Paz, Cristian
Burgos, Viviana
Menna-Barreto, Rubem F. S.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bombaça, Ana Cristina Souza
Dossow, Daniela Von
Barbosa, Juliana Magalhães Chaves
Paz, Cristian
Burgos, Viviana
Menna-Barreto, Rubem F. S.
dc.subject.other.pt_BR.fl_str_mv Trypanosomacruzi
Doença de Chagas
Quimioterapia
Autofagia
Mitocôndria
Sesquiterpenóides
topic Trypanosomacruzi
Doença de Chagas
Quimioterapia
Autofagia
Mitocôndria
Sesquiterpenóides
Trypanosomacruzi
Chagas Disease
Chemotherapy
Sesquiterpenoids
Mitochondria
Autophagy
dc.subject.en.pt_BR.fl_str_mv Trypanosomacruzi
Chagas Disease
Chemotherapy
Sesquiterpenoids
Mitochondria
Autophagy
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-08T18:01:54Z
dc.date.available.fl_str_mv 2019-02-08T18:01:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BOMBAÇA, Ana Cristina et al. TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosoma cruzi. Molecules, v. 23, n. 2800, p. 1-14, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31505
dc.identifier.issn.pt_BR.fl_str_mv 1420-3049
dc.identifier.doi.none.fl_str_mv 10.3390/molecules23112800
dc.identifier.eissn.none.fl_str_mv 1420-3049
identifier_str_mv BOMBAÇA, Ana Cristina et al. TrypanocidalActivity of Natural Sesquiterpenoids Involves Mitochondrial Dysfunction, ROS Production and Autophagic Phenotype in Trypanosoma cruzi. Molecules, v. 23, n. 2800, p. 1-14, 2018.
1420-3049
10.3390/molecules23112800
url https://www.arca.fiocruz.br/handle/icict/31505
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31505/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/31505/2/julianaMC_barbosa_etal_IOC_2018.pdf
https://www.arca.fiocruz.br/bitstream/icict/31505/3/julianaMC_barbosa_etal_IOC_2018.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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