Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection

Detalhes bibliográficos
Autor(a) principal: Almeida, Lucas Frederico de
Data de Publicação: 2017
Outros Autores: Silva, Juliana Almeida, Andrade, Viviane Magalhães, Machado, Paulo Roberto Lima, Jamieson, Sarra E, Carvalho Filho, Edgar Marcelino, Blackwell, Jenefer M, Castellucci, Léa Cristina de Carvalho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24893
Resumo: CARVALHOI FILHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Almeida, Lucas Frederico deSilva, Juliana AlmeidaAndrade, Viviane MagalhãesMachado, Paulo Roberto LimaJamieson, Sarra ECarvalho Filho, Edgar MarcelinoBlackwell, Jenefer MCastellucci, Léa Cristina de Carvalho2018-02-20T17:52:29Z2018-02-20T17:52:29Z2017ALMEIDA, L. F. et al. Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection. Infection, Genetics and Evolution, v. 49, p. 212–220, 2017.1567-1348https://www.arca.fiocruz.br/handle/icict/2489310.1016/j.meegid.2017.01.018CARVALHOI FILHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.theNIH Grant AI 30639 and CNPq/INCT.Federal University of Bahia. National Institute of Science and Technology in Tropical Diseases. Salvador, BA, Brazil / Federal University of Bahia. Post-graduation in Health Sciences. Salvador, BA, BrazilFederal University of Bahia. National Institute of Science and Technology in Tropical Diseases. Salvador, BA, Brazil / Federal University of Bahia. Post-graduation in Health Sciences. Salvador, BA, BrazilFederal University of Bahia. National Institute of Science and Technology in Tropical Diseases. Salvador, BA, Brazil / Federal University of Bahia. Post-graduation in Health Sciences. Salvador, BA, BrazilFederal University of Bahia. National Institute of Science and Technology in Tropical Diseases. Salvador, BA, BrazilThe University of Western Australia.Telethon Kids Institute. Subiaco, Western Australia, AustraliaFederal University of Bahia. National Institute of Science and Technology in Tropical Diseases. Salvador, BA, BrazilThe University of Western Australia.Telethon Kids Institute. Subiaco, Western Australia, Australia / University of Cambridge. Cambridge Institute for Medical Research. Department of Pathology. Cambridge, UKFederal University of Bahia. National Institute of Science and Technology in Tropical Diseases. Salvador, BA, BrazilFLI1 (Friend leukemia virus integration 1) and IL6 (interleukin 6; IL-6) are associated with Leishmania braziliensis susceptibility. Cutaneous lesions show exaggerated matrix metalloproteinase 1 (MMP1). In other skin diseases, FLI1 promoter methylation reduces FLI1 expression, and low FLI1 down-regulates MMP1. IL-6 increases FLI1 expression. We hypothesized that epigenetic regulation of FLI1 in cutaneous leishmaniasis, together with IL-6, might determine MMP1 expression. While generally low (<10%), percent FLI1 promoter methylation was lower (P=0.001) in lesion biopsies than normal skin. Contrary to expectation, a strong positive correlation occurred between FLI1 methylation and gene expression in lesions (r=0.98, P=0.0005) and in IL-6-treated L. braziliensis-infected macrophages (r=0.99, P=0.0004). In silico analysis of the FLI1 promoter revealed co-occurring active H3K27ac and repressive DNA methylation marks to enhance gene expression. FLI1 expression was enhanced between 3 and 24hour post infection in untreated (P=0.0002) and IL-6-treated (P=0.028) macrophages. MMP1 was enhanced in lesion biopsies (P=0.0002), induced (P=0.007) in infected macrophages, but strongly inhibited by IL-6. No correlations occurred between FLI1 and MMP1 expression in lesions or infected macrophages (with/without IL-6). We conclude that MMP1 is regulated by factors other than FLI1, and that the influence of IL-6 on MMP1 was independent of its effect on FLI1.engElsevierLeishmanioseFLI1MMP1IL-6Lesões cutâneasMacrófagosLeishmaniasisFLI1MMP1IL-6Cutaneous lesionsMacrophagesAnalysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24893/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAlmeida L Analysis of expression of FLI1 and MMP1.pdfAlmeida L Analysis of expression of FLI1 and 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dc.title.pt_BR.fl_str_mv Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection
title Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection
spellingShingle Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection
Almeida, Lucas Frederico de
Leishmaniose
FLI1
MMP1
IL-6
Lesões cutâneas
Macrófagos
Leishmaniasis
FLI1
MMP1
IL-6
Cutaneous lesions
Macrophages
title_short Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection
title_full Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection
title_fullStr Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection
title_full_unstemmed Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection
title_sort Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection
author Almeida, Lucas Frederico de
author_facet Almeida, Lucas Frederico de
Silva, Juliana Almeida
Andrade, Viviane Magalhães
Machado, Paulo Roberto Lima
Jamieson, Sarra E
Carvalho Filho, Edgar Marcelino
Blackwell, Jenefer M
Castellucci, Léa Cristina de Carvalho
author_role author
author2 Silva, Juliana Almeida
Andrade, Viviane Magalhães
Machado, Paulo Roberto Lima
Jamieson, Sarra E
Carvalho Filho, Edgar Marcelino
Blackwell, Jenefer M
Castellucci, Léa Cristina de Carvalho
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Lucas Frederico de
Silva, Juliana Almeida
Andrade, Viviane Magalhães
Machado, Paulo Roberto Lima
Jamieson, Sarra E
Carvalho Filho, Edgar Marcelino
Blackwell, Jenefer M
Castellucci, Léa Cristina de Carvalho
dc.subject.other.pt_BR.fl_str_mv Leishmaniose
FLI1
MMP1
IL-6
Lesões cutâneas
Macrófagos
topic Leishmaniose
FLI1
MMP1
IL-6
Lesões cutâneas
Macrófagos
Leishmaniasis
FLI1
MMP1
IL-6
Cutaneous lesions
Macrophages
dc.subject.en.pt_BR.fl_str_mv Leishmaniasis
FLI1
MMP1
IL-6
Cutaneous lesions
Macrophages
description CARVALHOI FILHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-20T17:52:29Z
dc.date.available.fl_str_mv 2018-02-20T17:52:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, L. F. et al. Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection. Infection, Genetics and Evolution, v. 49, p. 212–220, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24893
dc.identifier.issn.pt_BR.fl_str_mv 1567-1348
dc.identifier.doi.none.fl_str_mv 10.1016/j.meegid.2017.01.018
identifier_str_mv ALMEIDA, L. F. et al. Analysis of expression of FLI1 and MMP1 in American cutaneous leishmaniasis caused by Leishmania braziliensis infection. Infection, Genetics and Evolution, v. 49, p. 212–220, 2017.
1567-1348
10.1016/j.meegid.2017.01.018
url https://www.arca.fiocruz.br/handle/icict/24893
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/24893/1/license.txt
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