Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19323 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. |
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Porrozzi, RenatoCosta, Marcos V. Santos daTeva, AntonioFalqueto, AloísioFerreira, Adelson L.Santos, Claudiney D. dosFernandes, Ana PaulaGazzinelli, Ricardo T.Campos Neto, AntonioGrimaldi Jr., Gabriel2017-06-06T15:32:54Z2017-06-06T15:32:54Z2007PORROZZI, Renato; et al. Comparative Evaluation of Enzyme-Linked Immunosorbent Assays Based on Crude and Recombinant Leishmanial Antigens for Serodiagnosis of Symptomatic and Asymptomatic Leishmania infantum Visceral Infections in Dogs. Clinical and Vaccine Immunology, v.14, n.5, p.544-548, May 2007.1556-6811https://www.arca.fiocruz.br/handle/icict/1932310.1128/CVI.00420-061556-679XengAmerican Society for MicrobiologyCãesInfecções visceraisLeishmania infantumSorodiagnósticoImunossorvente ligado a enzimasAntígenosLeishmania infantumDogsVisceral InfectionsSerodiagnosisEnzyme-Linked ImmunosorbentAntigensComparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade Federal do Espírito Santo. Vitória, ES, Brasil.,Universidade Federal do Espírito Santo. Vitória, ES, Brasil.,Universidade Federal do Espírito Santo. Vitória, ES, Brasil.,Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.The Forsyth Institute. Boston, Massachusetts, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.The diagnosis of visceral leishmaniasis remains difficult in rural areas where the disease is endemic, and serologic methods still need assessment, as they are not very sensitive for the detection of asymptomatic infectious dogs. Here we present data on the development of enzyme-linked immunosorbent assay (ELISA)-based methods for the detection of antibodies against recombinant leishmanial antigens (namely, the recombinant K26 [rK26] and rK39 antigens from Leishmania infantum and the rA2 protein from Leishmania donovani) in comparison to ELISAs employing crude soluble antigen (CSA). The assays utilized sera from known negative controls (n=25) and clinically asymptomatic (n=50) and symptomatic (n=50) dogs with confirmed L. infantum infections. Additional studies were also done using sera from animals harboring other infections (n=14) for the evaluation of cross-reactivity. Our study indicated that rK26 and rK39 used in ELISAs provided very high sensitivities for the detection of symptomatic dogs (94% and 100%, respectively), followed by CSA (88%) and rA2 (70%). Conversely, rA2 was more sensitive for asymptomatic dogs (88%) than rK39 and rK26 (both 66%) and CSA (30%). Some cross-reactivity in sera from dogs with other infections (Leishmania braziliensis and Leptospira interrogans) was identified, but the rA2 protein provided the greatest specificity (98%). Data further indicate that all three recombinant proteins must be used in parallel to detect essentially all infected dogs. Efforts should be made to develop a cheap and reliable serologic test based on epitope selection from these diagnostic markers for the sensitive detection of L. infantum-infected dogs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19323/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALgabriel_grimaldijr3_etal_IOC_2007.pdfgabriel_grimaldijr3_etal_IOC_2007.pdfapplication/pdf95256https://www.arca.fiocruz.br/bitstream/icict/19323/2/gabriel_grimaldijr3_etal_IOC_2007.pdf176d81f8be2c9f63b7c5ead7f0265bdbMD52TEXTgabriel_grimaldijr3_etal_IOC_2007.pdf.txtgabriel_grimaldijr3_etal_IOC_2007.pdf.txtExtracted texttext/plain35573https://www.arca.fiocruz.br/bitstream/icict/19323/3/gabriel_grimaldijr3_etal_IOC_2007.pdf.txt2bb4f71081309723476fea1e5efcc28fMD53icict/193232022-06-24 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| dc.title.pt_BR.fl_str_mv |
Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs |
| title |
Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs |
| spellingShingle |
Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs Porrozzi, Renato Cães Infecções viscerais Leishmania infantum Sorodiagnóstico Imunossorvente ligado a enzimas Antígenos Leishmania infantum Dogs Visceral Infections Serodiagnosis Enzyme-Linked Immunosorbent Antigens |
| title_short |
Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs |
| title_full |
Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs |
| title_fullStr |
Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs |
| title_full_unstemmed |
Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs |
| title_sort |
Comparative evaluation of enzyme-linked immunosorbent assays based on crude and recombinant leishmanial antigens for serodiagnosis of symptomatic and asymptomatic Leishmania infantum visceral infections in dogs |
| author |
Porrozzi, Renato |
| author_facet |
Porrozzi, Renato Costa, Marcos V. Santos da Teva, Antonio Falqueto, Aloísio Ferreira, Adelson L. Santos, Claudiney D. dos Fernandes, Ana Paula Gazzinelli, Ricardo T. Campos Neto, Antonio Grimaldi Jr., Gabriel |
| author_role |
author |
| author2 |
Costa, Marcos V. Santos da Teva, Antonio Falqueto, Aloísio Ferreira, Adelson L. Santos, Claudiney D. dos Fernandes, Ana Paula Gazzinelli, Ricardo T. Campos Neto, Antonio Grimaldi Jr., Gabriel |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Porrozzi, Renato Costa, Marcos V. Santos da Teva, Antonio Falqueto, Aloísio Ferreira, Adelson L. Santos, Claudiney D. dos Fernandes, Ana Paula Gazzinelli, Ricardo T. Campos Neto, Antonio Grimaldi Jr., Gabriel |
| dc.subject.other.pt_BR.fl_str_mv |
Cães Infecções viscerais Leishmania infantum Sorodiagnóstico Imunossorvente ligado a enzimas Antígenos |
| topic |
Cães Infecções viscerais Leishmania infantum Sorodiagnóstico Imunossorvente ligado a enzimas Antígenos Leishmania infantum Dogs Visceral Infections Serodiagnosis Enzyme-Linked Immunosorbent Antigens |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania infantum Dogs Visceral Infections Serodiagnosis Enzyme-Linked Immunosorbent Antigens |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. |
| publishDate |
2007 |
| dc.date.issued.fl_str_mv |
2007 |
| dc.date.accessioned.fl_str_mv |
2017-06-06T15:32:54Z |
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2017-06-06T15:32:54Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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| dc.identifier.citation.fl_str_mv |
PORROZZI, Renato; et al. Comparative Evaluation of Enzyme-Linked Immunosorbent Assays Based on Crude and Recombinant Leishmanial Antigens for Serodiagnosis of Symptomatic and Asymptomatic Leishmania infantum Visceral Infections in Dogs. Clinical and Vaccine Immunology, v.14, n.5, p.544-548, May 2007. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/19323 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1556-6811 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/CVI.00420-06 |
| dc.identifier.eissn.none.fl_str_mv |
1556-679X |
| identifier_str_mv |
PORROZZI, Renato; et al. Comparative Evaluation of Enzyme-Linked Immunosorbent Assays Based on Crude and Recombinant Leishmanial Antigens for Serodiagnosis of Symptomatic and Asymptomatic Leishmania infantum Visceral Infections in Dogs. Clinical and Vaccine Immunology, v.14, n.5, p.544-548, May 2007. 1556-6811 10.1128/CVI.00420-06 1556-679X |
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https://www.arca.fiocruz.br/handle/icict/19323 |
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eng |
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eng |
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openAccess |
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American Society for Microbiology |
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American Society for Microbiology |
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