Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy

Detalhes bibliográficos
Autor(a) principal: Sousa, Karina P. de
Data de Publicação: 2023
Outros Autores: Rossi, Izadora, Abdullahi, Mahamed, Ramirez, Marcel Ivan, Stratton, Dan, Inal, Jameel Malhador
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57396
Resumo: Bioscience Research Group. School of Life and Medical Sciences. University of Hertfordshire. Hertfordshire, UK.
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spelling Sousa, Karina P. deRossi, IzadoraAbdullahi, MahamedRamirez, Marcel IvanStratton, DanInal, Jameel Malhador2023-03-15T19:48:33Z2023-03-15T19:48:33Z2023SOUSA, Karina P. de et al. Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy. WIREs Nanomed Nanobiotechnol, v. 15, n. e1835, p. 1–29, 2023.1939-0041https://www.arca.fiocruz.br/handle/icict/5739610.1002/wnan.1835porWileyIsolamento CelularExtracellular VesiclesBiomarkersCell SeparationPathology, MolecularVesículas ExtracelularesBiomarcadoresSeparación CelularPatología MolecularVésicules extracellulairesMarqueurs biologiquesSéparation cellulaireAnatomopathologie moléculaireVesículas ExtracelularesBiomarcadoresSeparação CelularPatologia MolecularIsolation and characterization of extracellular vesicles and future directions in diagnosis and therapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBioscience Research Group. School of Life and Medical Sciences. University of Hertfordshire. Hertfordshire, UK.School of Human Sciences. London Metropolitan University. London, UK. / Universidade Federal do Paraná. Curitiba, PR, Brasil.School of Human Sciences. London Metropolitan University. London, UK.Universidade Federal do Paraná. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Open University. The School of Life, Health, and Chemical Sciences. Milton Keynes, UK.Bioscience Research Group. School of Life and Medical Sciences. University of Hertfordshire. Hertfordshire, UK. / School of Human Sciences. London Metropolitan University. London, UK.Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV-related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57396/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALWIREs_Nanomed - 2022.pdfWIREs_Nanomed - 2022.pdfapplication/pdf4007592https://www.arca.fiocruz.br/bitstream/icict/57396/2/WIREs_Nanomed%20-%202022.pdf7017c4429b1c24e739231f92c37a7d72MD52icict/573962023-03-15 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dc.title.en_US.fl_str_mv Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
title Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
spellingShingle Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
Sousa, Karina P. de
Isolamento Celular
Extracellular Vesicles
Biomarkers
Cell Separation
Pathology, Molecular
Vesículas Extracelulares
Biomarcadores
Separación Celular
Patología Molecular
Vésicules extracellulaires
Marqueurs biologiques
Séparation cellulaire
Anatomopathologie moléculaire
Vesículas Extracelulares
Biomarcadores
Separação Celular
Patologia Molecular
title_short Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
title_full Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
title_fullStr Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
title_full_unstemmed Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
title_sort Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy
author Sousa, Karina P. de
author_facet Sousa, Karina P. de
Rossi, Izadora
Abdullahi, Mahamed
Ramirez, Marcel Ivan
Stratton, Dan
Inal, Jameel Malhador
author_role author
author2 Rossi, Izadora
Abdullahi, Mahamed
Ramirez, Marcel Ivan
Stratton, Dan
Inal, Jameel Malhador
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Karina P. de
Rossi, Izadora
Abdullahi, Mahamed
Ramirez, Marcel Ivan
Stratton, Dan
Inal, Jameel Malhador
dc.subject.other.en_US.fl_str_mv Isolamento Celular
topic Isolamento Celular
Extracellular Vesicles
Biomarkers
Cell Separation
Pathology, Molecular
Vesículas Extracelulares
Biomarcadores
Separación Celular
Patología Molecular
Vésicules extracellulaires
Marqueurs biologiques
Séparation cellulaire
Anatomopathologie moléculaire
Vesículas Extracelulares
Biomarcadores
Separação Celular
Patologia Molecular
dc.subject.en.en_US.fl_str_mv Extracellular Vesicles
Biomarkers
Cell Separation
Pathology, Molecular
dc.subject.es.en_US.fl_str_mv Vesículas Extracelulares
Biomarcadores
Separación Celular
Patología Molecular
dc.subject.fr.en_US.fl_str_mv Vésicules extracellulaires
Marqueurs biologiques
Séparation cellulaire
Anatomopathologie moléculaire
dc.subject.decs.en_US.fl_str_mv Vesículas Extracelulares
Biomarcadores
Separação Celular
Patologia Molecular
description Bioscience Research Group. School of Life and Medical Sciences. University of Hertfordshire. Hertfordshire, UK.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-03-15T19:48:33Z
dc.date.available.fl_str_mv 2023-03-15T19:48:33Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUSA, Karina P. de et al. Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy. WIREs Nanomed Nanobiotechnol, v. 15, n. e1835, p. 1–29, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57396
dc.identifier.issn.en_US.fl_str_mv 1939-0041
dc.identifier.doi.none.fl_str_mv 10.1002/wnan.1835
identifier_str_mv SOUSA, Karina P. de et al. Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy. WIREs Nanomed Nanobiotechnol, v. 15, n. e1835, p. 1–29, 2023.
1939-0041
10.1002/wnan.1835
url https://www.arca.fiocruz.br/handle/icict/57396
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/57396/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/57396/2/WIREs_Nanomed%20-%202022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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