Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice

Detalhes bibliográficos
Autor(a) principal: Lopera, Damaris
Data de Publicação: 2011
Outros Autores: Naranjo, Tonny W., Cruz, Oswaldo Gonçalves, Restrepo, Angela, Cano, Elena, Lenzi, Henrique Leonel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1371/journal.pntd.0001232
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3709
Resumo: Conceived and designed the experiments: LEC HLL DL TWN. Performed the experiments: DL TWN. Analyzed the data: DL HLL OGC LEC. Contributed reagents/materials/analysis tools: HLL. Wrote the paper: DL HLL AR. Scientific advisor of the work who reviewed the manuscript: AR LEC.
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spelling Lopera, DamarisNaranjo, Tonny W.Cruz, Oswaldo GonçalvesRestrepo, AngelaCano, ElenaLenzi, Henrique Leonel2011-12-13T16:38:11Z2011-12-13T16:38:11Z2011LOPERA, Damaris et al. Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice. Plos Neglected Tropical Diseases, v. 5, p. 1-9, 2011.1935-2727https://www.arca.fiocruz.br/handle/icict/370910.1371/journal.pntd.0001232Conceived and designed the experiments: LEC HLL DL TWN. Performed the experiments: DL TWN. Analyzed the data: DL HLL OGC LEC. Contributed reagents/materials/analysis tools: HLL. Wrote the paper: DL HLL AR. Scientific advisor of the work who reviewed the manuscript: AR LEC.Corporación para Investigaciones Biológicas. Medical and Experimental Mycology Group. Medellín, Colômbia.Corporación para Investigaciones Biológicas. Medical and Experimental Mycology Group, Medellín, Colômbia / Universidad Pontificia Bolivarina. School of Health Sciences. Medellín, Colômbia.Fundação Oswaldo Cruz. Presidência. Programa de Computação Científica.Rio de Janeiro, Brasil.Corporación para Investigaciones Biológicas. Medical and Experimental Mycology Group. Medellín, Colômbia.Corporación para Investigaciones Biológicas. Medical and Experimental Mycology Group. Medellín, Colômbia / Universidad Pontificia Bolivarina. School of Health Sciences. Medellín, Colômbia / Universidad de Antioquia. Microbiology School. Medellín, Colômbia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Background: Paracoccidioidomycosis (PCM), an endemic systemic mycosis caused by the fungus Paracoccidioides brasiliensis (Pb), usually results in severe lung damage in patients. Methods and Findings: Considering the difficulties to sequentially study the infection in humans, this work was done in mice inoculated intranasally with infective Pb-conidia. Lungs of control and Pb-infected mice were studied after 2-hours, 4, 8, 12 and 16-weeks post-infection (p.i) in order to define histopathologic patterns of pulmonary lesions, multiplex-cytokine profiles and their dynamics during the course of this mycosis. Besides the nodular/granulomatous lesions previously informed, results revealed additional non-formerly described lung abnormalities, such as periarterial sheath inflammation and pseudotumoral masses. The following chronologic stages occurring during the course of the experimental infection were defined: Stage one (2-hours p.i): mild septal infiltration composed by neutrophils and macrophages accompanied by an intense ‘‘cytokine burst’’ represented by significant increases in IL-1a, IL-1b, IL-4, IL-5, IL-6, IL-10, IL12p70, IL-13, IL-17, Eotaxin, G-CSF, MCP1, MIP1a, GM-CSF, IFN-c, MIP1b and TNFa levels. Stage two (4-weeks p.i): presence of nodules, evidence of incipient periarterial- and intense but disperse parenchymal- inflammation, abnormalities that continued to be accompanied by hyper-secretion of those cytokines and chemokines mentioned in the first stage of infection. Stages three and four (8 and 12-weeks p.i.): fungal proliferation, inflammation and collagenesis reached their highest intensity with particular involvement of the periarterial space. Paradoxically, lung cytokines and chemokines were down-regulated with significant decreases in IL-2,IL-3,IL-5,IL-9,IL-13,IL-15,GM-CSF,IFN-c,MIP1b and TNFa. Stage five (16-weeks p.i.): inflammation decreased becoming limited to the pseudotumoral masses and was accompanied by a ‘‘silent’’ cytokine response, except for PDGF, MIG, RANTES and IL12p40 which remained up-regulated for the duration of the experiment. Conclusions: Results of this study identified both classic and novel patterns corresponding to histopathologic and immunologic responses occurring during the course of experimental PCM.engParacoccidioidomicoseDoenças EndêmicasParacoccidioidesParacoccidioidomycosisEndemic Systemic MycosisParacoccidioides brasiliensisParacoccidioidomicoseDoenças EndêmicasParacoccidioidesStructural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALStructural and Topographic Dynamics of Pulmonary.pdfStructural and Topographic Dynamics of Pulmonary.pdfapplication/pdf7833246https://www.arca.fiocruz.br/bitstream/icict/3709/1/Structural%20and%20Topographic%20Dynamics%20of%20Pulmonary.pdf97e7a0133244a5af523b7a4de5e60cb4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3709/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTStructural and Topographic Dynamics of Pulmonary.pdf.txtStructural and Topographic Dynamics of Pulmonary.pdf.txtExtracted texttext/plain45646https://www.arca.fiocruz.br/bitstream/icict/3709/5/Structural%20and%20Topographic%20Dynamics%20of%20Pulmonary.pdf.txt651ae7438cd236790891861a90cdbec3MD55THUMBNAILStructural and Topographic Dynamics of Pulmonary.pdf.jpgStructural and Topographic Dynamics of Pulmonary.pdf.jpgGenerated Thumbnailimage/jpeg2615https://www.arca.fiocruz.br/bitstream/icict/3709/4/Structural%20and%20Topographic%20Dynamics%20of%20Pulmonary.pdf.jpgf8a25fa07f7dff43efb5f34273affc86MD54icict/37092019-06-24 22:02:01.63oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-25T01:02:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice
title Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice
spellingShingle Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice
Lopera, Damaris
Paracoccidioidomicose
Doenças Endêmicas
Paracoccidioides
Paracoccidioidomycosis
Endemic Systemic Mycosis
Paracoccidioides brasiliensis
Paracoccidioidomicose
Doenças Endêmicas
Paracoccidioides
title_short Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice
title_full Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice
title_fullStr Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice
title_full_unstemmed Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice
title_sort Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice
author Lopera, Damaris
author_facet Lopera, Damaris
Naranjo, Tonny W.
Cruz, Oswaldo Gonçalves
Restrepo, Angela
Cano, Elena
Lenzi, Henrique Leonel
author_role author
author2 Naranjo, Tonny W.
Cruz, Oswaldo Gonçalves
Restrepo, Angela
Cano, Elena
Lenzi, Henrique Leonel
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lopera, Damaris
Naranjo, Tonny W.
Cruz, Oswaldo Gonçalves
Restrepo, Angela
Cano, Elena
Lenzi, Henrique Leonel
dc.subject.other.pt_BR.fl_str_mv Paracoccidioidomicose
Doenças Endêmicas
Paracoccidioides
topic Paracoccidioidomicose
Doenças Endêmicas
Paracoccidioides
Paracoccidioidomycosis
Endemic Systemic Mycosis
Paracoccidioides brasiliensis
Paracoccidioidomicose
Doenças Endêmicas
Paracoccidioides
dc.subject.en.pt_BR.fl_str_mv Paracoccidioidomycosis
Endemic Systemic Mycosis
Paracoccidioides brasiliensis
dc.subject.decs.pt_BR.fl_str_mv Paracoccidioidomicose
Doenças Endêmicas
Paracoccidioides
description Conceived and designed the experiments: LEC HLL DL TWN. Performed the experiments: DL TWN. Analyzed the data: DL HLL OGC LEC. Contributed reagents/materials/analysis tools: HLL. Wrote the paper: DL HLL AR. Scientific advisor of the work who reviewed the manuscript: AR LEC.
publishDate 2011
dc.date.accessioned.fl_str_mv 2011-12-13T16:38:11Z
dc.date.available.fl_str_mv 2011-12-13T16:38:11Z
dc.date.issued.fl_str_mv 2011
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LOPERA, Damaris et al. Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice. Plos Neglected Tropical Diseases, v. 5, p. 1-9, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3709
dc.identifier.issn.none.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001232
identifier_str_mv LOPERA, Damaris et al. Structural and Topographic Dynamics of Pulmonary Histopathology and Local Cytokine Profiles in Paracoccidioides brasiliensis Conidia-Infected Mice. Plos Neglected Tropical Diseases, v. 5, p. 1-9, 2011.
1935-2727
10.1371/journal.pntd.0001232
url https://www.arca.fiocruz.br/handle/icict/3709
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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