Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.

Detalhes bibliográficos
Autor(a) principal: Fernandes, Consuelo Barreto
Data de Publicação: 2014
Outros Autores: Magalhães Junior, Jairo Torres, Jesus, Clauceane de, Souza, Bárbara Maria Paraná da Silva, Larangeira, Daniela Farias, Fraga, Deborah Bittencourt Mothé, Veras, Patrícia Sampaio Tavares, Melo, Stella Maria Barrouin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7722
Resumo: Federal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, Brasil
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spelling Fernandes, Consuelo BarretoMagalhães Junior, Jairo TorresJesus, Clauceane deSouza, Bárbara Maria Paraná da SilvaLarangeira, Daniela FariasFraga, Deborah Bittencourt MothéVeras, Patrícia Sampaio TavaresMelo, Stella Maria Barrouin2014-05-29T16:52:55Z2014-05-29T16:52:55Z2014FERNANDES, C. B. et al. Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. Vaccine, v. 32, n. 11, p. 1287-1295, 2014.1873-2518https://www.arca.fiocruz.br/handle/icict/7722engElsevier LtdComparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, BrasilFederal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, BrasilFederal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, BrasilLaboratory of Cellular and Molecular Biology. HOSPMEV. UFBA. Salvador, BA, BrasilFederal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPME V). Salvador, BA, Brasil / UFBA. Departament of Veterinary Anatomy. Pathology and Clinics of the School of Veterinary Medicine and Zootechny. Salvador, BA, BrasilUFBA. Departament of Preventive Veterinary Medicine and Animal Production of the School of Veterinary Medicine and Zootechny. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, Brasil / UFBA. Departament of Preventive Veterinary Medicine and Animal Production of the School of Veterinary Medicine and Zootechny. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilBACKGROUND: The incidence of zoonotic canine visceral leishmaniasis (CVL) would decrease if dogs were effectively vaccinated; however, additional data on the efficacy of canine vaccines are required for their approved preventative use. PURPOSE: To prospectively evaluate vaccination outcomes using two products commercially available in Brazil, with respect to adverse reactions (reactogenicity), humoral response, disease signs, parasitism, and parasite infectiousness in naturally exposed pet dogs in an endemic area of visceral leishmaniasis (VL). METHODS: From 2010 to 2012, healthy dogs were vaccinated with Leishmune(®) (50 animals) or Leish-Tec(®) (50 animals). Each dog was examined to identify clinical signs during peri- and post-vaccination procedures every 2 months for 11 months to identify the presence of parasites or parasite DNA in splenic samples using culturing or PCR, respectively. Levels of anti-Leishmania IgG, IgG1, and IgG2 were quantified in sera by ELISA and infectiousness was assessed by xenodiagnosis. RESULTS: Adverse effects occurred in 2.2% (1/45) and 13.0% (6/46) of the animals in the Leishmune(®) and Leish-Tec(®) groups, respectively. IgG levels peaked on the 21st day following the first dose of Leishmune(®) and on the 21st day after the second dose of Leish-Tec(®). The final seropositivity rate for IgG was 32.5% (13/40) and 30.9% (13/42) in the Leishmune(®) and Leish-Tec(®) groups, respectively. The Leishmune(®) group presented higher levels of IgG1 and IgG2 compared to the Leish-Tec(®) group (p<0.001), and ELISA reactivity in both vaccinated groups was significantly lower (p<0.001) than in infected positive control dogs. Parasitism was observed in 12.2% (5/41) of the Leishmune(®) group, and 7.9% (3/38) of the Leish-Tec(®) group, with xenodiagnostic transmission rates of Leishmania to Lutzomyia longipalpis of 5.1% (2/39), and 5.4% (2/37), respectively. CONCLUSIONS: No significant differences were observed in dogs vaccinated with Leishmune(®) or Leish-Tec(®), with respect to LVC clinical aspects, parasitism, IgG seropositivity, or dog infectiousness. The Leishmune(®)-vaccinated animals presented higher levels of IgG, IgG1, and IgG2. 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dc.title.pt_BR.fl_str_mv Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.
title Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.
spellingShingle Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.
Fernandes, Consuelo Barreto
Canine immunization
Leishmune® and Leish-Tec®
Reactogenicity
Leishmania infantum
Xenodiagnosis
Lutzomyia longipalpis
title_short Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.
title_full Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.
title_fullStr Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.
title_full_unstemmed Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.
title_sort Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.
author Fernandes, Consuelo Barreto
author_facet Fernandes, Consuelo Barreto
Magalhães Junior, Jairo Torres
Jesus, Clauceane de
Souza, Bárbara Maria Paraná da Silva
Larangeira, Daniela Farias
Fraga, Deborah Bittencourt Mothé
Veras, Patrícia Sampaio Tavares
Melo, Stella Maria Barrouin
author_role author
author2 Magalhães Junior, Jairo Torres
Jesus, Clauceane de
Souza, Bárbara Maria Paraná da Silva
Larangeira, Daniela Farias
Fraga, Deborah Bittencourt Mothé
Veras, Patrícia Sampaio Tavares
Melo, Stella Maria Barrouin
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Consuelo Barreto
Magalhães Junior, Jairo Torres
Jesus, Clauceane de
Souza, Bárbara Maria Paraná da Silva
Larangeira, Daniela Farias
Fraga, Deborah Bittencourt Mothé
Veras, Patrícia Sampaio Tavares
Melo, Stella Maria Barrouin
dc.subject.en.pt_BR.fl_str_mv Canine immunization
Leishmune® and Leish-Tec®
Reactogenicity
Leishmania infantum
Xenodiagnosis
Lutzomyia longipalpis
topic Canine immunization
Leishmune® and Leish-Tec®
Reactogenicity
Leishmania infantum
Xenodiagnosis
Lutzomyia longipalpis
description Federal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, Brasil
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-05-29T16:52:55Z
dc.date.available.fl_str_mv 2014-05-29T16:52:55Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FERNANDES, C. B. et al. Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. Vaccine, v. 32, n. 11, p. 1287-1295, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7722
dc.identifier.issn.none.fl_str_mv 1873-2518
identifier_str_mv FERNANDES, C. B. et al. Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. Vaccine, v. 32, n. 11, p. 1287-1295, 2014.
1873-2518
url https://www.arca.fiocruz.br/handle/icict/7722
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
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