Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7722 |
Resumo: | Federal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, Brasil |
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Fernandes, Consuelo BarretoMagalhães Junior, Jairo TorresJesus, Clauceane deSouza, Bárbara Maria Paraná da SilvaLarangeira, Daniela FariasFraga, Deborah Bittencourt MothéVeras, Patrícia Sampaio TavaresMelo, Stella Maria Barrouin2014-05-29T16:52:55Z2014-05-29T16:52:55Z2014FERNANDES, C. B. et al. Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. Vaccine, v. 32, n. 11, p. 1287-1295, 2014.1873-2518https://www.arca.fiocruz.br/handle/icict/7722engElsevier LtdComparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, BrasilFederal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, BrasilFederal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, BrasilLaboratory of Cellular and Molecular Biology. HOSPMEV. UFBA. Salvador, BA, BrasilFederal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPME V). Salvador, BA, Brasil / UFBA. Departament of Veterinary Anatomy. Pathology and Clinics of the School of Veterinary Medicine and Zootechny. Salvador, BA, BrasilUFBA. Departament of Preventive Veterinary Medicine and Animal Production of the School of Veterinary Medicine and Zootechny. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, Brasil / UFBA. Departament of Preventive Veterinary Medicine and Animal Production of the School of Veterinary Medicine and Zootechny. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilBACKGROUND: The incidence of zoonotic canine visceral leishmaniasis (CVL) would decrease if dogs were effectively vaccinated; however, additional data on the efficacy of canine vaccines are required for their approved preventative use. PURPOSE: To prospectively evaluate vaccination outcomes using two products commercially available in Brazil, with respect to adverse reactions (reactogenicity), humoral response, disease signs, parasitism, and parasite infectiousness in naturally exposed pet dogs in an endemic area of visceral leishmaniasis (VL). METHODS: From 2010 to 2012, healthy dogs were vaccinated with Leishmune(®) (50 animals) or Leish-Tec(®) (50 animals). Each dog was examined to identify clinical signs during peri- and post-vaccination procedures every 2 months for 11 months to identify the presence of parasites or parasite DNA in splenic samples using culturing or PCR, respectively. Levels of anti-Leishmania IgG, IgG1, and IgG2 were quantified in sera by ELISA and infectiousness was assessed by xenodiagnosis. RESULTS: Adverse effects occurred in 2.2% (1/45) and 13.0% (6/46) of the animals in the Leishmune(®) and Leish-Tec(®) groups, respectively. IgG levels peaked on the 21st day following the first dose of Leishmune(®) and on the 21st day after the second dose of Leish-Tec(®). The final seropositivity rate for IgG was 32.5% (13/40) and 30.9% (13/42) in the Leishmune(®) and Leish-Tec(®) groups, respectively. The Leishmune(®) group presented higher levels of IgG1 and IgG2 compared to the Leish-Tec(®) group (p<0.001), and ELISA reactivity in both vaccinated groups was significantly lower (p<0.001) than in infected positive control dogs. Parasitism was observed in 12.2% (5/41) of the Leishmune(®) group, and 7.9% (3/38) of the Leish-Tec(®) group, with xenodiagnostic transmission rates of Leishmania to Lutzomyia longipalpis of 5.1% (2/39), and 5.4% (2/37), respectively. CONCLUSIONS: No significant differences were observed in dogs vaccinated with Leishmune(®) or Leish-Tec(®), with respect to LVC clinical aspects, parasitism, IgG seropositivity, or dog infectiousness. The Leishmune(®)-vaccinated animals presented higher levels of IgG, IgG1, and IgG2. The animals vaccinated with Leish-Tec(®) exhibited adverse reactions with greater frequency and severity.Canine immunizationLeishmune® and Leish-Tec®ReactogenicityLeishmania infantumXenodiagnosisLutzomyia longipalpisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFernandes CB Comparison of two....pdfFernandes CB Comparison of two....pdfapplication/pdf1344041https://www.arca.fiocruz.br/bitstream/icict/7722/1/Fernandes%20CB%20Comparison%20of%20two....pdf2122a7970195d89d9565fe8291d03648MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7722/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTFernandes CB Comparison of two....pdf.txtFernandes CB Comparison of two....pdf.txtExtracted texttext/plain58825https://www.arca.fiocruz.br/bitstream/icict/7722/5/Fernandes%20CB%20Comparison%20of%20two....pdf.txtd7bad2871652bdd1027175afe96292edMD55THUMBNAILFernandes CB Comparison of two....pdf.jpgFernandes CB Comparison of two....pdf.jpgGenerated Thumbnailimage/jpeg2116https://www.arca.fiocruz.br/bitstream/icict/7722/4/Fernandes%20CB%20Comparison%20of%20two....pdf.jpg3f202312015264b2c8766bbd4726c143MD54icict/77222023-03-15 14:33:21.976oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. |
title |
Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. |
spellingShingle |
Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. Fernandes, Consuelo Barreto Canine immunization Leishmune® and Leish-Tec® Reactogenicity Leishmania infantum Xenodiagnosis Lutzomyia longipalpis |
title_short |
Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. |
title_full |
Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. |
title_fullStr |
Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. |
title_full_unstemmed |
Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. |
title_sort |
Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. |
author |
Fernandes, Consuelo Barreto |
author_facet |
Fernandes, Consuelo Barreto Magalhães Junior, Jairo Torres Jesus, Clauceane de Souza, Bárbara Maria Paraná da Silva Larangeira, Daniela Farias Fraga, Deborah Bittencourt Mothé Veras, Patrícia Sampaio Tavares Melo, Stella Maria Barrouin |
author_role |
author |
author2 |
Magalhães Junior, Jairo Torres Jesus, Clauceane de Souza, Bárbara Maria Paraná da Silva Larangeira, Daniela Farias Fraga, Deborah Bittencourt Mothé Veras, Patrícia Sampaio Tavares Melo, Stella Maria Barrouin |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, Consuelo Barreto Magalhães Junior, Jairo Torres Jesus, Clauceane de Souza, Bárbara Maria Paraná da Silva Larangeira, Daniela Farias Fraga, Deborah Bittencourt Mothé Veras, Patrícia Sampaio Tavares Melo, Stella Maria Barrouin |
dc.subject.en.pt_BR.fl_str_mv |
Canine immunization Leishmune® and Leish-Tec® Reactogenicity Leishmania infantum Xenodiagnosis Lutzomyia longipalpis |
topic |
Canine immunization Leishmune® and Leish-Tec® Reactogenicity Leishmania infantum Xenodiagnosis Lutzomyia longipalpis |
description |
Federal University of Bahia (UFBA). Laboratory of Veterinary Infectious Diseases. Hospital of Veterinary Medicine (HOSPMEV). Salvador, BA, Brasil |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-05-29T16:52:55Z |
dc.date.available.fl_str_mv |
2014-05-29T16:52:55Z |
dc.date.issued.fl_str_mv |
2014 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FERNANDES, C. B. et al. Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. Vaccine, v. 32, n. 11, p. 1287-1295, 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/7722 |
dc.identifier.issn.none.fl_str_mv |
1873-2518 |
identifier_str_mv |
FERNANDES, C. B. et al. Comparison of two commercial vaccines against visceral leishmaniasis in dogs from endemic areas: IgG, and subclasses, parasitism, and parasite transmission by xenodiagnosis. Vaccine, v. 32, n. 11, p. 1287-1295, 2014. 1873-2518 |
url |
https://www.arca.fiocruz.br/handle/icict/7722 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier Ltd |
publisher.none.fl_str_mv |
Elsevier Ltd |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) |
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