Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Smircich, Pablo
Data de Publicação: 2015
Outros Autores: Eastman, Guillermo, Bispo, Saloe, Duhagon, María Ana, Guerra‑Slompo, Eloise Pavão, Garat, Beatriz, Goldenberg, Samuel, Munroe, David J., Dallagiovanna, Bruno, Holetz, Fabíola Barbieri, Sotelo-Silveira, Jose
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/22788
Resumo: Laboratory of Molecular Interactions. School of Sciences. Universidad de la República. Montevideo, Uruguay / Department of Genetics. School of Medicine. Universidad de la República. Montevideo, Uruguay.
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spelling Smircich, PabloEastman, GuillermoBispo, SaloeDuhagon, María AnaGuerra‑Slompo, Eloise PavãoGarat, BeatrizGoldenberg, SamuelMunroe, David J.Dallagiovanna, BrunoHoletz, Fabíola BarbieriSotelo-Silveira, Jose2017-10-05T17:51:25Z2017-10-05T17:51:25Z2015SMIRCICH, Pablo et al. Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi. BMC Genomics, v. 16, n. 443, p. 1-14, 2015.1471-2164https://www.arca.fiocruz.br/handle/icict/2278810.1186/s12864-015-1563-8porBioMed CentralPMC4460968Trypanosoma cruziNeglected DiseasesTranscriptomeRibosomesGene Expression RegulationEnfermedades DesatendidasRibosomasRegulación de la Expresión GénicaDoenças NegligenciadasTranscriptomaRibossomosRegulação da Expressão GênicaRibosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratory of Molecular Interactions. School of Sciences. Universidad de la República. Montevideo, Uruguay / Department of Genetics. School of Medicine. Universidad de la República. Montevideo, Uruguay.Department of Genomics. Instituto de Investigaciones Biológicas Clemente Estable. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Laboratory of Molecular Interactions. School of Sciences. Universidad de la República. Montevideo, Uruguay / Department of Genetics. School of Medicine. Universidad de la República. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Laboratory of Molecular Interactions. School of Sciences. Universidad de la República. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Cancer Research Technology Program. Leidos Biomedical Research, Inc. Frederick National Laboratory for Cancer Research. Frederick, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Department of Genomics. Instituto de Investigaciones Biológicas Clemente Estable. Montevideo, Uruguay / Department of Cell and Molecular Biology. School of Sciences. Universidad de la Republica. Montevideo, Uruguay.Due to the absence of transcription initiation regulation of protein coding genes transcribed by RNA polymerase II, posttranscriptional regulation is responsible for the majority of gene expression changes in trypanosomatids. Therefore, cataloging the abundance of mRNAs (transcriptome) and the level of their translation (translatome) is a key step to understand control of gene expression in these organisms. Here we assess the extent of regulation of the transcriptome and the translatome in the Chagas disease causing agent, Trypanosoma cruzi, in both the non-infective (epimastigote) and infective (metacyclic trypomastigote) insect’s life stages using RNA-seq and ribosome profiling. The observed steady state transcript levels support constitutive transcription and maturation implying the existence of distinctive posttranscriptional regulatory mechanisms controlling gene expression levels at those parasite stages. Meanwhile, the downregulation of a large proportion of the translatome indicates a key role of translation control in differentiation into the infective form. The previously described proteomic data correlate better with the translatomes than with the transcriptomes and translational efficiency analysis shows a wide dynamic range, reinforcing the importance of translatability as a regulatory step. Translation efficiencies for protein families like ribosomal components are diminished while translation of the transialidase virulence factors is upregulated in the quiescent infective metacyclic trypomastigote stage.A large subset of genes is modulated at the translation level in two different stages of Trypanosoma cruzi life cycle. Translation upregulation of virulence factors and downregulation of ribosomal proteins indicates different degrees of control operating to prepare the parasite for an infective life form. Taking together our results show that translational regulation, in addition to regulation of steady state level of mRNA, is a major factor playing a role during the parasite differentiation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/22788/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALSmircichRibosomeok.pdfSmircichRibosomeok.pdfapplication/pdf1932166https://www.arca.fiocruz.br/bitstream/icict/22788/2/SmircichRibosomeok.pdff86cd5c629d96acf4ddaa98cb466a641MD52TEXTSmircichRibosomeok.pdf.txtSmircichRibosomeok.pdf.txtExtracted texttext/plain60447https://www.arca.fiocruz.br/bitstream/icict/22788/3/SmircichRibosomeok.pdf.txt1df61fc9183ab639d4e76784c7f86dc8MD53icict/227882022-01-12 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dc.title.pt_BR.fl_str_mv Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi
title Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi
spellingShingle Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi
Smircich, Pablo
PMC4460968
Trypanosoma cruzi
Neglected Diseases
Transcriptome
Ribosomes
Gene Expression Regulation
Enfermedades Desatendidas
Ribosomas
Regulación de la Expresión Génica
Doenças Negligenciadas
Transcriptoma
Ribossomos
Regulação da Expressão Gênica
title_short Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi
title_full Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi
title_fullStr Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi
title_full_unstemmed Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi
title_sort Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi
author Smircich, Pablo
author_facet Smircich, Pablo
Eastman, Guillermo
Bispo, Saloe
Duhagon, María Ana
Guerra‑Slompo, Eloise Pavão
Garat, Beatriz
Goldenberg, Samuel
Munroe, David J.
Dallagiovanna, Bruno
Holetz, Fabíola Barbieri
Sotelo-Silveira, Jose
author_role author
author2 Eastman, Guillermo
Bispo, Saloe
Duhagon, María Ana
Guerra‑Slompo, Eloise Pavão
Garat, Beatriz
Goldenberg, Samuel
Munroe, David J.
Dallagiovanna, Bruno
Holetz, Fabíola Barbieri
Sotelo-Silveira, Jose
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Smircich, Pablo
Eastman, Guillermo
Bispo, Saloe
Duhagon, María Ana
Guerra‑Slompo, Eloise Pavão
Garat, Beatriz
Goldenberg, Samuel
Munroe, David J.
Dallagiovanna, Bruno
Holetz, Fabíola Barbieri
Sotelo-Silveira, Jose
dc.subject.mesh.pt_BR.fl_str_mv PMC4460968
topic PMC4460968
Trypanosoma cruzi
Neglected Diseases
Transcriptome
Ribosomes
Gene Expression Regulation
Enfermedades Desatendidas
Ribosomas
Regulación de la Expresión Génica
Doenças Negligenciadas
Transcriptoma
Ribossomos
Regulação da Expressão Gênica
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
dc.subject.en.pt_BR.fl_str_mv Neglected Diseases
Transcriptome
Ribosomes
Gene Expression Regulation
dc.subject.es.pt_BR.fl_str_mv Enfermedades Desatendidas
Ribosomas
Regulación de la Expresión Génica
dc.subject.decs.pt_BR.fl_str_mv Doenças Negligenciadas
Transcriptoma
Ribossomos
Regulação da Expressão Gênica
description Laboratory of Molecular Interactions. School of Sciences. Universidad de la República. Montevideo, Uruguay / Department of Genetics. School of Medicine. Universidad de la República. Montevideo, Uruguay.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2017-10-05T17:51:25Z
dc.date.available.fl_str_mv 2017-10-05T17:51:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SMIRCICH, Pablo et al. Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi. BMC Genomics, v. 16, n. 443, p. 1-14, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/22788
dc.identifier.issn.pt_BR.fl_str_mv 1471-2164
dc.identifier.doi.none.fl_str_mv 10.1186/s12864-015-1563-8
identifier_str_mv SMIRCICH, Pablo et al. Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi. BMC Genomics, v. 16, n. 443, p. 1-14, 2015.
1471-2164
10.1186/s12864-015-1563-8
url https://www.arca.fiocruz.br/handle/icict/22788
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
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