Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells

Detalhes bibliográficos
Autor(a) principal: Pires, Bruno R. B.
Data de Publicação: 2021
Outros Autores: Binato, Renata, Ferreira, Gerson M., Corrêa, Stephany, Du Rocher, Bárbara, Bulzico, Daniel, Crocamo, Susanne, Santos, Everton Cruz dos, Lima, Luize G., Abdelhay, Eliana Saul Furquim Werneck
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50843
Resumo: Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.
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spelling Pires, Bruno R. B.Binato, RenataFerreira, Gerson M.Corrêa, StephanyDu Rocher, BárbaraBulzico, DanielCrocamo, SusanneSantos, Everton Cruz dosLima, Luize G.Abdelhay, Eliana Saul Furquim Werneck2022-01-21T14:08:07Z2022-01-21T14:08:07Z2021PIRES, Bruno R. B. et al. Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells. International Journal of Molecular Sciences, v. 22, 12144, p. 1 - 17, Nov. 2021.1422-0067https://www.arca.fiocruz.br/handle/icict/5084310.3390/ijms222212144engMDPICâncer de MamaHER2Expressão geneticaIL17Twist1Breast cancerHER2Twist1IL17Gene expressionTwist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Unidade de Endocrinologia Oncológica. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Núcleo de Pesquisa Clínica, Hospital de Câncer III. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Tumour Microenvironment Laboratory, QIMR Berghofer Medical Research Institute. Herston, QLD 4006, Australia.Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Abstract: Breast cancer (BC) is a heterogeneous disease composed of multiple subtypes with different molecular characteristics and clinical outcomes. The metastatic process in BC depends on the transcription factors (TFs) related to epithelial-mesenchymal transition (EMT), including the master regulator Twist1. However, its role beyond EMT in BC subtypes remains unclear. Our study aimed to investigate the role of Twist1, beyond EMT, in the molecular subtypes of BC. In patients, we observed the overexpression of TWIST1 in the HER2+ group. The silencing of TWIST1 in HER2+ BC cells resulted in the upregulation of 138 genes and the downregulation of 174 genes compared to control cells in a microarray assay. In silico analysis revealed correlations between Twist1 and important biological processes such as the Th17-mediated immune response, suggesting that Twist1 could be relevant for IL-17 signaling in HER2+ BC. IL-17 signaling was then examined, and it was shown that TWIST1 knockdown caused the downregulation of leading members of IL-17 signaling pathway. Taken together, our findings suggest that Twist1 plays a role on IL-17 signaling in HER2+ BC.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50843/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBarbaraDu_Rocher_etal_IOC_2021.pdfBarbaraDu_Rocher_etal_IOC_2021.pdfapplication/pdf3920290https://www.arca.fiocruz.br/bitstream/icict/50843/2/BarbaraDu_Rocher_etal_IOC_2021.pdfda04334dab230a1a1b5576940e9d3323MD52icict/508432022-03-24 15:12:34.495oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-03-24T18:12:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
spellingShingle Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
Pires, Bruno R. B.
Câncer de Mama
HER2
Expressão genetica
IL17
Twist1
Breast cancer
HER2
Twist1
IL17
Gene expression
title_short Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_full Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_fullStr Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_full_unstemmed Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
title_sort Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
author Pires, Bruno R. B.
author_facet Pires, Bruno R. B.
Binato, Renata
Ferreira, Gerson M.
Corrêa, Stephany
Du Rocher, Bárbara
Bulzico, Daniel
Crocamo, Susanne
Santos, Everton Cruz dos
Lima, Luize G.
Abdelhay, Eliana Saul Furquim Werneck
author_role author
author2 Binato, Renata
Ferreira, Gerson M.
Corrêa, Stephany
Du Rocher, Bárbara
Bulzico, Daniel
Crocamo, Susanne
Santos, Everton Cruz dos
Lima, Luize G.
Abdelhay, Eliana Saul Furquim Werneck
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pires, Bruno R. B.
Binato, Renata
Ferreira, Gerson M.
Corrêa, Stephany
Du Rocher, Bárbara
Bulzico, Daniel
Crocamo, Susanne
Santos, Everton Cruz dos
Lima, Luize G.
Abdelhay, Eliana Saul Furquim Werneck
dc.subject.other.pt_BR.fl_str_mv Câncer de Mama
HER2
Expressão genetica
IL17
Twist1
topic Câncer de Mama
HER2
Expressão genetica
IL17
Twist1
Breast cancer
HER2
Twist1
IL17
Gene expression
dc.subject.en.pt_BR.fl_str_mv Breast cancer
HER2
Twist1
IL17
Gene expression
description Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-01-21T14:08:07Z
dc.date.available.fl_str_mv 2022-01-21T14:08:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PIRES, Bruno R. B. et al. Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells. International Journal of Molecular Sciences, v. 22, 12144, p. 1 - 17, Nov. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50843
dc.identifier.issn.pt_BR.fl_str_mv 1422-0067
dc.identifier.doi.none.fl_str_mv 10.3390/ijms222212144
identifier_str_mv PIRES, Bruno R. B. et al. Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells. International Journal of Molecular Sciences, v. 22, 12144, p. 1 - 17, Nov. 2021.
1422-0067
10.3390/ijms222212144
url https://www.arca.fiocruz.br/handle/icict/50843
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50843/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50843/2/BarbaraDu_Rocher_etal_IOC_2021.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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