Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/50843 |
Resumo: | Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil. |
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Pires, Bruno R. B.Binato, RenataFerreira, Gerson M.Corrêa, StephanyDu Rocher, BárbaraBulzico, DanielCrocamo, SusanneSantos, Everton Cruz dosLima, Luize G.Abdelhay, Eliana Saul Furquim Werneck2022-01-21T14:08:07Z2022-01-21T14:08:07Z2021PIRES, Bruno R. B. et al. Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells. International Journal of Molecular Sciences, v. 22, 12144, p. 1 - 17, Nov. 2021.1422-0067https://www.arca.fiocruz.br/handle/icict/5084310.3390/ijms222212144engMDPICâncer de MamaHER2Expressão geneticaIL17Twist1Breast cancerHER2Twist1IL17Gene expressionTwist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Unidade de Endocrinologia Oncológica. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Núcleo de Pesquisa Clínica, Hospital de Câncer III. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Tumour Microenvironment Laboratory, QIMR Berghofer Medical Research Institute. Herston, QLD 4006, Australia.Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil.Abstract: Breast cancer (BC) is a heterogeneous disease composed of multiple subtypes with different molecular characteristics and clinical outcomes. The metastatic process in BC depends on the transcription factors (TFs) related to epithelial-mesenchymal transition (EMT), including the master regulator Twist1. However, its role beyond EMT in BC subtypes remains unclear. Our study aimed to investigate the role of Twist1, beyond EMT, in the molecular subtypes of BC. In patients, we observed the overexpression of TWIST1 in the HER2+ group. The silencing of TWIST1 in HER2+ BC cells resulted in the upregulation of 138 genes and the downregulation of 174 genes compared to control cells in a microarray assay. In silico analysis revealed correlations between Twist1 and important biological processes such as the Th17-mediated immune response, suggesting that Twist1 could be relevant for IL-17 signaling in HER2+ BC. IL-17 signaling was then examined, and it was shown that TWIST1 knockdown caused the downregulation of leading members of IL-17 signaling pathway. Taken together, our findings suggest that Twist1 plays a role on IL-17 signaling in HER2+ BC.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50843/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBarbaraDu_Rocher_etal_IOC_2021.pdfBarbaraDu_Rocher_etal_IOC_2021.pdfapplication/pdf3920290https://www.arca.fiocruz.br/bitstream/icict/50843/2/BarbaraDu_Rocher_etal_IOC_2021.pdfda04334dab230a1a1b5576940e9d3323MD52icict/508432022-03-24 15:12:34.495oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-03-24T18:12:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells |
title |
Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells |
spellingShingle |
Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells Pires, Bruno R. B. Câncer de Mama HER2 Expressão genetica IL17 Twist1 Breast cancer HER2 Twist1 IL17 Gene expression |
title_short |
Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells |
title_full |
Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells |
title_fullStr |
Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells |
title_full_unstemmed |
Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells |
title_sort |
Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells |
author |
Pires, Bruno R. B. |
author_facet |
Pires, Bruno R. B. Binato, Renata Ferreira, Gerson M. Corrêa, Stephany Du Rocher, Bárbara Bulzico, Daniel Crocamo, Susanne Santos, Everton Cruz dos Lima, Luize G. Abdelhay, Eliana Saul Furquim Werneck |
author_role |
author |
author2 |
Binato, Renata Ferreira, Gerson M. Corrêa, Stephany Du Rocher, Bárbara Bulzico, Daniel Crocamo, Susanne Santos, Everton Cruz dos Lima, Luize G. Abdelhay, Eliana Saul Furquim Werneck |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pires, Bruno R. B. Binato, Renata Ferreira, Gerson M. Corrêa, Stephany Du Rocher, Bárbara Bulzico, Daniel Crocamo, Susanne Santos, Everton Cruz dos Lima, Luize G. Abdelhay, Eliana Saul Furquim Werneck |
dc.subject.other.pt_BR.fl_str_mv |
Câncer de Mama HER2 Expressão genetica IL17 Twist1 |
topic |
Câncer de Mama HER2 Expressão genetica IL17 Twist1 Breast cancer HER2 Twist1 IL17 Gene expression |
dc.subject.en.pt_BR.fl_str_mv |
Breast cancer HER2 Twist1 IL17 Gene expression |
description |
Instituto Nacional de Câncer José Alencar Gomes da Silva. Laboratório de Célula-Tronco. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia para o Controle do Câncer. Rio de Janeiro, RJ, Brasil. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021 |
dc.date.accessioned.fl_str_mv |
2022-01-21T14:08:07Z |
dc.date.available.fl_str_mv |
2022-01-21T14:08:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PIRES, Bruno R. B. et al. Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells. International Journal of Molecular Sciences, v. 22, 12144, p. 1 - 17, Nov. 2021. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/50843 |
dc.identifier.issn.pt_BR.fl_str_mv |
1422-0067 |
dc.identifier.doi.none.fl_str_mv |
10.3390/ijms222212144 |
identifier_str_mv |
PIRES, Bruno R. B. et al. Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells. International Journal of Molecular Sciences, v. 22, 12144, p. 1 - 17, Nov. 2021. 1422-0067 10.3390/ijms222212144 |
url |
https://www.arca.fiocruz.br/handle/icict/50843 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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MDPI |
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MDPI |
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