Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides

Detalhes bibliográficos
Autor(a) principal: Maracahipes, Álan C.
Data de Publicação: 2019
Outros Autores: Taveira, Gabriel B., Mello, Erica O., Carvalho, André O., Rodrigues, Rosana, Perales, Jonas, Ferreira, André Teixeira, Silva, Marciele S., Rocha, Gustavo L., Fernandes, Kátia Valevski Sales, Gomes, Valdirene M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35038
Resumo: Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.
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spelling Maracahipes, Álan C.Taveira, Gabriel B.Mello, Erica O.Carvalho, André O.Rodrigues, RosanaPerales, JonasFerreira, André TeixeiraSilva, Marciele S.Rocha, Gustavo L.Fernandes, Kátia Valevski SalesGomes, Valdirene M.2019-08-23T17:09:13Z2019-08-23T17:09:13Z2019MARACAHIPES, Álan C. et al. Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides. Bioscience Reports, v. 39, p. 1-14, 2019.0144-8463https://www.arca.fiocruz.br/handle/icict/3503810.1042/BSR201818891573-4935engPortland PressColletotrichum gloeosporioidesAnálise bioquímicaPeptídeos antimicrobianosGenótipos de capsicumInfecção de frutasColletotrichum gloeosporioidesBiochemical analysisAntimicrobial peptidesCapsicum genotypesFruit infectionBiochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioidesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Ciêencias e Tecnologias Agropecúarias. Laboratório de Mehoramento e Genética Vegetal. Campos de Goytacazes, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Laboratório de Química e Função de Proteínas e Pept[ideos. Campos de Goytacazes, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Laboratório de Química e Função de Proteínas e Pept[ideos. Campos de Goytacazes, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.There are several phytosanitary problems that have been causing serious damage to the Capsicum crops, including anthracnose. Upon attack by certain pathogens, various protein molecules are produced, which are known as proteins related to pathogenesis (PR proteins), including antimicrobial peptides such as protease inhibitors, defensins and lipid transfer proteins (LTPs). The objective of this work is to identify antimicrobial proteins and/or peptides of two genotypes from Capsicum annuum fruits infected with Colletotrichum gloeosporioides The fungus was inoculated into Capsicum fruits by the deposition of a spore suspension (106 conidia ml-1), and after 24 and 48 h intervals, the fruits were removed from the humid chamber and subjected to a protein extraction process. Protein analysis of the extracts was performed by tricine gel electrophoresis and Western blotting. The distinctive bands between genotypes in the electrophoresis profiles were subjected to mass spectrometry sequencing. Trypsin inhibition assays, reverse zymographic detection of protease inhibition and β-1,3-glucanase activity assays were also performed and extracts were also tested for their ability to inhibit the growth of C. gloeosporioides fungi 'in vitro' There were several low molecular weight proteins in all treated samples, and some treatments in which antimicrobial peptides such as defensin, lipid transfer protein (LTP) and protease inhibitor have been identified. It was shown that the green fruits are more responsive to infection, showing the production of antimicrobial peptides in response to injury and inoculation of the fungus, what did not occur in ripe fruits under any treatment.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35038/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL2 - AndreTeixeira_JonasPerales_etal_IOC_2019.pdf2 - AndreTeixeira_JonasPerales_etal_IOC_2019.pdfapplication/pdf1428078https://www.arca.fiocruz.br/bitstream/icict/35038/2/2%20-%20AndreTeixeira_JonasPerales_etal_IOC_2019.pdf4ec1a26843546337ee12894ea30f1043MD52TEXT2 - AndreTeixeira_JonasPerales_etal_IOC_2019.pdf.txt2 - AndreTeixeira_JonasPerales_etal_IOC_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides
title Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides
spellingShingle Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides
Maracahipes, Álan C.
Colletotrichum gloeosporioides
Análise bioquímica
Peptídeos antimicrobianos
Genótipos de capsicum
Infecção de frutas
Colletotrichum gloeosporioides
Biochemical analysis
Antimicrobial peptides
Capsicum genotypes
Fruit infection
title_short Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides
title_full Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides
title_fullStr Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides
title_full_unstemmed Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides
title_sort Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides
author Maracahipes, Álan C.
author_facet Maracahipes, Álan C.
Taveira, Gabriel B.
Mello, Erica O.
Carvalho, André O.
Rodrigues, Rosana
Perales, Jonas
Ferreira, André Teixeira
Silva, Marciele S.
Rocha, Gustavo L.
Fernandes, Kátia Valevski Sales
Gomes, Valdirene M.
author_role author
author2 Taveira, Gabriel B.
Mello, Erica O.
Carvalho, André O.
Rodrigues, Rosana
Perales, Jonas
Ferreira, André Teixeira
Silva, Marciele S.
Rocha, Gustavo L.
Fernandes, Kátia Valevski Sales
Gomes, Valdirene M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Maracahipes, Álan C.
Taveira, Gabriel B.
Mello, Erica O.
Carvalho, André O.
Rodrigues, Rosana
Perales, Jonas
Ferreira, André Teixeira
Silva, Marciele S.
Rocha, Gustavo L.
Fernandes, Kátia Valevski Sales
Gomes, Valdirene M.
dc.subject.other.pt_BR.fl_str_mv Colletotrichum gloeosporioides
Análise bioquímica
Peptídeos antimicrobianos
Genótipos de capsicum
Infecção de frutas
topic Colletotrichum gloeosporioides
Análise bioquímica
Peptídeos antimicrobianos
Genótipos de capsicum
Infecção de frutas
Colletotrichum gloeosporioides
Biochemical analysis
Antimicrobial peptides
Capsicum genotypes
Fruit infection
dc.subject.en.pt_BR.fl_str_mv Colletotrichum gloeosporioides
Biochemical analysis
Antimicrobial peptides
Capsicum genotypes
Fruit infection
description Universidade Estadual do Norte Fluminense Darcy Ribeiro. Centro de Biociências e Biotecnologia. Laboratório de Fisiologia e Bioquímica de Microrganismos. Campos dos Goytacazes, RJ, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-08-23T17:09:13Z
dc.date.available.fl_str_mv 2019-08-23T17:09:13Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARACAHIPES, Álan C. et al. Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides. Bioscience Reports, v. 39, p. 1-14, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35038
dc.identifier.issn.pt_BR.fl_str_mv 0144-8463
dc.identifier.doi.none.fl_str_mv 10.1042/BSR20181889
dc.identifier.eissn.none.fl_str_mv 1573-4935
identifier_str_mv MARACAHIPES, Álan C. et al. Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides. Bioscience Reports, v. 39, p. 1-14, 2019.
0144-8463
10.1042/BSR20181889
1573-4935
url https://www.arca.fiocruz.br/handle/icict/35038
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Portland Press
publisher.none.fl_str_mv Portland Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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