Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.

Soto, Manuel; Corvo, Laura; Garde, Esther; Ramírez, Laura; Iniesta, Virginia; Bonay, Pedro; Gómez Nieto, Carlos; González, Victor M; Martín, M. Elena; Alonso, Carlos; Coelho, Eduardo Antonio Ferraz; Barral, Aldina Maria Prado; Barral Netto, Manoel; Iborra, Salvador

Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.

Detalhes bibliográficos
Autor(a) principal:	Soto, Manuel
Data de Publicação:	2015
Outros Autores:	Corvo, Laura, Garde, Esther, Ramírez, Laura, Iniesta, Virginia, Bonay, Pedro, Gómez Nieto, Carlos, González, Victor M, Martín, M. Elena, Alonso, Carlos, Coelho, Eduardo Antonio Ferraz, Barral, Aldina Maria Prado, Barral Netto, Manoel, Iborra, Salvador
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
DOI:	10.371/journal.pntd.003751
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/11198
Resumo:	Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, Spain

Metadados do item

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spelling	Soto, ManuelCorvo, LauraGarde, EstherRamírez, LauraIniesta, VirginiaBonay, PedroGómez Nieto, CarlosGonzález, Victor MMartín, M. ElenaAlonso, CarlosCoelho, Eduardo Antonio FerrazBarral, Aldina Maria PradoBarral Netto, ManoelIborra, Salvador2015-07-09T17:05:39Z2015-07-09T17:05:39Z2015SOTO, M. et al. Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice. PLoS Neglected Tropical Diseases, v. 9, n. 5, p. e0003751, 2015.1935-2735https://www.arca.fiocruz.br/handle/icict/1119810.371/journal.pntd.003751engPublic Library of ScienceLeishmania InfantumLeishmania majorProteinasVacinacaoHumanosCoadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, SpainUniversidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, SpainUniversidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, SpainUniversidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, SpainUniversity of Extrema. Leishman Ceres Laboratory (GLP Compliance Certified) Parasitology. Caceres, SpainUniversidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, SpainUniversity of Extrema. Leishman Ceres Laboratory (GLP Compliance Certified) Parasitology. Caceres, SpainInstituto Ralmon y Cajal de Investigacion Sanitaria (IRYCIS). Hospital Ramon y Cajal. Departamento de Bioquimica-Investigacion. Madrid, SpainInstituto Ralmon y Cajal de Investigacion Sanitaria (IRYCIS). Hospital Ramon y Cajal. Departamento de Bioquimica-Investigacion. Madrid, SpainUniversidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, SpainUniversidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Patologia Clinica. COLTEC. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCentro Nacional de Investigaciones Cardiovasculares. Department of Vascular Biology and Inflammation. Immunobiology of Inflammation Laboratory. Madrid, SpainBACKGROUND: Highly conserved intracellular proteins from Leishmania have been described as antigens in natural and experimental infected mammals. The present study aimed to evaluate the antigenicity and prophylactic properties of the Leishmania infantum Poly (A) binding proteins (LiPABPs). METHODOLOGY/PRINCIPAL FINDINGS: Three different members of the LiPABP family have been described. Recombinant tools based on these proteins were constructed: recombinant proteins and DNA vaccines. The three recombinant proteins were employed for coating ELISA plates. Sera from human and canine patients of visceral leishmaniasis and human patients of mucosal leishmaniasis recognized the three LiPABPs. In addition, the protective efficacy of a DNA vaccine based on the combination of the three Leishmania PABPs has been tested in a model of progressive murine leishmaniasis: BALB/c mice infected with Leishmania major. The induction of a Th1-like response against the LiPABP family by genetic vaccination was able to down-regulate the IL-10 predominant responses elicited by parasite LiPABPs after infection in this murine model. This modulation resulted in a partial protection against L. major infection. LiPABP vaccinated mice showed a reduction on the pathology that was accompanied by a decrease in parasite burdens, in antibody titers against Leishmania antigens and in the IL-4 and IL-10 parasite-specific mediated responses in comparison to control mice groups immunized with saline or with the non-recombinant plasmid. CONCLUSION/SIGNIFICANCE: The results presented here demonstrate for the first time the prophylactic properties of a new family of Leishmania antigenic intracellular proteins, the LiPABPs. The redirection of the immune response elicited against the LiPABP family (from IL-10 towards IFN-Î³ mediated responses) by genetic vaccination was able to induce a partial protection against the development of the disease in a highly susceptible murine model of leishmaniasis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/11198/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALSoto M Coadministration....pdfSoto M Coadministration....pdfapplication/pdf2183707https://www.arca.fiocruz.br/bitstream/icict/11198/2/Soto%20M%20Coadministration....pdfa9609272b81f00c7f67e25d8ad23fcfdMD52TEXTSoto M Coadministration....pdf.txtSoto M Coadministration....pdf.txtExtracted texttext/plain87163https://www.arca.fiocruz.br/bitstream/icict/11198/3/Soto%20M%20Coadministration....pdf.txta39dd112c297fb28423a301dfc6709e1MD53icict/111982023-03-15 14:33:11.98oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:11Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.
title	Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.
spellingShingle	Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice. Soto, Manuel Leishmania Infantum Leishmania major Proteinas Vacinacao Humanos
title_short	Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.
title_full	Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.
title_fullStr	Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.
title_full_unstemmed	Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.
title_sort	Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.
author	Soto, Manuel
author_facet	Soto, Manuel Corvo, Laura Garde, Esther Ramírez, Laura Iniesta, Virginia Bonay, Pedro Gómez Nieto, Carlos González, Victor M Martín, M. Elena Alonso, Carlos Coelho, Eduardo Antonio Ferraz Barral, Aldina Maria Prado Barral Netto, Manoel Iborra, Salvador
author_role	author
author2	Corvo, Laura Garde, Esther Ramírez, Laura Iniesta, Virginia Bonay, Pedro Gómez Nieto, Carlos González, Victor M Martín, M. Elena Alonso, Carlos Coelho, Eduardo Antonio Ferraz Barral, Aldina Maria Prado Barral Netto, Manoel Iborra, Salvador
author2_role	author author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Soto, Manuel Corvo, Laura Garde, Esther Ramírez, Laura Iniesta, Virginia Bonay, Pedro Gómez Nieto, Carlos González, Victor M Martín, M. Elena Alonso, Carlos Coelho, Eduardo Antonio Ferraz Barral, Aldina Maria Prado Barral Netto, Manoel Iborra, Salvador
dc.subject.other.pt_BR.fl_str_mv	Leishmania Infantum Leishmania major Proteinas Vacinacao Humanos
topic	Leishmania Infantum Leishmania major Proteinas Vacinacao Humanos
description	Universidad Autónoma de Madrid. Centro de Biología Molecular Severo Ochoa (CSIC-UAM). Departamento de Biología Molecular. Madrid, Spain
publishDate	2015
dc.date.accessioned.fl_str_mv	2015-07-09T17:05:39Z
dc.date.available.fl_str_mv	2015-07-09T17:05:39Z
dc.date.issued.fl_str_mv	2015
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	SOTO, M. et al. Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice. PLoS Neglected Tropical Diseases, v. 9, n. 5, p. e0003751, 2015.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/11198
dc.identifier.issn.none.fl_str_mv	1935-2735
dc.identifier.doi.none.fl_str_mv	10.371/journal.pntd.003751
identifier_str_mv	SOTO, M. et al. Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice. PLoS Neglected Tropical Diseases, v. 9, n. 5, p. e0003751, 2015. 1935-2735 10.371/journal.pntd.003751
url	https://www.arca.fiocruz.br/handle/icict/11198
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Public Library of Science
publisher.none.fl_str_mv	Public Library of Science
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/11198/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/11198/2/Soto%20M%20Coadministration....pdf https://www.arca.fiocruz.br/bitstream/icict/11198/3/Soto%20M%20Coadministration....pdf.txt
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bitstream.checksumAlgorithm.fl_str_mv	MD5 MD5 MD5
repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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Coadministration of the Three Antigenic Leishmania infantum Poly (A) Binding Proteins as a DNA Vaccine Induces Protection against Leishmania major Infection in BALB/c Mice.

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