Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil

Detalhes bibliográficos
Autor(a) principal: Blanton, Ronald Edward
Data de Publicação: 2011
Outros Autores: Blank, Walter A, Costa, Jackson Mauricio Lopes, Carmo, Theomira Mauadie de Azevedo, Reis, Eliana Almeida Gomes, Silva, Luciano Kalabric, Barbosa, Lúcio Macedo, Test, Matthew R., Reis, Mitermayer Galvão dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7499
Resumo: Case Western Reserve University. Centre for Global Health and Diseases. Cleveland, OH, USA
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spelling Blanton, Ronald EdwardBlank, Walter ACosta, Jackson Mauricio LopesCarmo, Theomira Mauadie de AzevedoReis, Eliana Almeida GomesSilva, Luciano KalabricBarbosa, Lúcio MacedoTest, Matthew R.Reis, Mitermayer Galvão dos2014-04-07T12:45:07Z2014-04-07T12:45:07Z2011BLANTON, R. E. et al. Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil. International Journal of Parasitology, v. 41, n. 10, p. 1093-1099, 2011.1879-0135https://www.arca.fiocruz.br/handle/icict/749910.1016/j.ijpara.2011.06.002.engNational Institute of HealthSchistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCase Western Reserve University. Centre for Global Health and Diseases. Cleveland, OH, USACase Western Reserve University. Centre for Global Health and Diseases. Cleveland, OH, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilCase Western Reserve University. Centre for Global Health and Diseases. Cleveland, OH, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilPraziquantel has been used to treat schistosome infections since 1979 and currently is the only chemotherapeutic agent in production for this purpose, raising concerns about the potential for the emergence of drug resistance. In practice, 10–20% of infected patients will continue to excrete eggs after treatment. It is not understood to what degree this represents selection of a resistant population or incomplete elimination due to the presence of immature worms at the time of treatment. We used a population genetics approach to test whether or not persistent Schistosoma mansoni parasites were drawn from the same population as susceptible parasites. In this study, stool samples were collected from 96% of individuals in two small Brazilian communities (populations 482 and 367) and examined for S. mansoni eggs. The combined prevalence of S. mansoni infections in the villages was 41%. Total egg DNA was extracted from each sample and was genotyped at 15 microsatellite markers. Day-to-day variation of the infrapopulation from an individual human host was low (median differentiation using Jost’s D = 0.010), so that a single stool was representative of the genotypes present in stool eggs, at least in the short term. Average pairwise analysis of D among all pre-treatment infrapopulations suggested moderate differentiation (mean D = 0.082 and 0.122 for the two villages), whereas the pre-treatment component population differentiation between the two communities was 0.047. The differentiation of the component population remaining after treatment from the fully susceptible component population was low (mean D = 0.007 and 0.020 for the two villages), suggesting that the persistent parasites were not selected by praziquantel treatment. We will continue to follow these communities for evidence of selection or changes in population structure.PraziquantelResistanceMicrosatellitePopulation geneticsSamplingGenetic differentiationSelectionAnti-Helmínticos/administração & dosagemResistência a MedicamentosPraziquantel/administração & dosagemSchistosoma mansoni/classificaçãoSchistosoma mansoni/efeitos de drogasEsquistossomose mansoni/quimioterapiaSeleção GenéticaAdolescenteAdultoIdosoIdoso de 80 anos ou maisAnimaisBrasilCriançaFezes/parasitologiaPré-EscolarFemininoGenótipoHumanosRepetições de MicrossatélitesLactenteMasculinoSchistosoma mansoni/genéticaAdulto-Joveminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBlanton R Schistosoma mansoni....pdfBlanton R Schistosoma mansoni....pdfapplication/pdf534314https://www.arca.fiocruz.br/bitstream/icict/7499/1/Blanton%20R%20Schistosoma%20mansoni....pdfb462e402a3a16c6957ffdf39836e2c06MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7499/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBlanton R Schistosoma mansoni....pdf.txtBlanton R Schistosoma mansoni....pdf.txtExtracted texttext/plain48786https://www.arca.fiocruz.br/bitstream/icict/7499/5/Blanton%20R%20Schistosoma%20mansoni....pdf.txtf9d0aa34f6b9d028f08da9028ccf8a16MD55THUMBNAILBlanton R Schistosoma mansoni....pdf.jpgBlanton R Schistosoma mansoni....pdf.jpgGenerated Thumbnailimage/jpeg2327https://www.arca.fiocruz.br/bitstream/icict/7499/4/Blanton%20R%20Schistosoma%20mansoni....pdf.jpg9d3e8db35e419cd25c6dee5ac4792bf5MD54icict/74992023-03-15 14:33:14.814oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:14Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil
title Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil
spellingShingle Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil
Blanton, Ronald Edward
Praziquantel
Resistance
Microsatellite
Population genetics
Sampling
Genetic differentiation
Selection
Anti-Helmínticos/administração & dosagem
Resistência a Medicamentos
Praziquantel/administração & dosagem
Schistosoma mansoni/classificação
Schistosoma mansoni/efeitos de drogas
Esquistossomose mansoni/quimioterapia
Seleção Genética
Adolescente
Adulto
Idoso
Idoso de 80 anos ou mais
Animais
Brasil
Criança
Fezes/parasitologia
Pré-Escolar
Feminino
Genótipo
Humanos
Repetições de Microssatélites
Lactente
Masculino
Schistosoma mansoni/genética
Adulto-Jovem
title_short Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil
title_full Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil
title_fullStr Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil
title_full_unstemmed Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil
title_sort Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil
author Blanton, Ronald Edward
author_facet Blanton, Ronald Edward
Blank, Walter A
Costa, Jackson Mauricio Lopes
Carmo, Theomira Mauadie de Azevedo
Reis, Eliana Almeida Gomes
Silva, Luciano Kalabric
Barbosa, Lúcio Macedo
Test, Matthew R.
Reis, Mitermayer Galvão dos
author_role author
author2 Blank, Walter A
Costa, Jackson Mauricio Lopes
Carmo, Theomira Mauadie de Azevedo
Reis, Eliana Almeida Gomes
Silva, Luciano Kalabric
Barbosa, Lúcio Macedo
Test, Matthew R.
Reis, Mitermayer Galvão dos
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Blanton, Ronald Edward
Blank, Walter A
Costa, Jackson Mauricio Lopes
Carmo, Theomira Mauadie de Azevedo
Reis, Eliana Almeida Gomes
Silva, Luciano Kalabric
Barbosa, Lúcio Macedo
Test, Matthew R.
Reis, Mitermayer Galvão dos
dc.subject.en.pt_BR.fl_str_mv Praziquantel
Resistance
Microsatellite
Population genetics
Sampling
Genetic differentiation
Selection
topic Praziquantel
Resistance
Microsatellite
Population genetics
Sampling
Genetic differentiation
Selection
Anti-Helmínticos/administração & dosagem
Resistência a Medicamentos
Praziquantel/administração & dosagem
Schistosoma mansoni/classificação
Schistosoma mansoni/efeitos de drogas
Esquistossomose mansoni/quimioterapia
Seleção Genética
Adolescente
Adulto
Idoso
Idoso de 80 anos ou mais
Animais
Brasil
Criança
Fezes/parasitologia
Pré-Escolar
Feminino
Genótipo
Humanos
Repetições de Microssatélites
Lactente
Masculino
Schistosoma mansoni/genética
Adulto-Jovem
dc.subject.decs.pt_BR.fl_str_mv Anti-Helmínticos/administração & dosagem
Resistência a Medicamentos
Praziquantel/administração & dosagem
Schistosoma mansoni/classificação
Schistosoma mansoni/efeitos de drogas
Esquistossomose mansoni/quimioterapia
Seleção Genética
Adolescente
Adulto
Idoso
Idoso de 80 anos ou mais
Animais
Brasil
Criança
Fezes/parasitologia
Pré-Escolar
Feminino
Genótipo
Humanos
Repetições de Microssatélites
Lactente
Masculino
Schistosoma mansoni/genética
Adulto-Jovem
description Case Western Reserve University. Centre for Global Health and Diseases. Cleveland, OH, USA
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-04-07T12:45:07Z
dc.date.available.fl_str_mv 2014-04-07T12:45:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BLANTON, R. E. et al. Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil. International Journal of Parasitology, v. 41, n. 10, p. 1093-1099, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7499
dc.identifier.issn.none.fl_str_mv 1879-0135
dc.identifier.doi.none.fl_str_mv 10.1016/j.ijpara.2011.06.002.
identifier_str_mv BLANTON, R. E. et al. Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil. International Journal of Parasitology, v. 41, n. 10, p. 1093-1099, 2011.
1879-0135
10.1016/j.ijpara.2011.06.002.
url https://www.arca.fiocruz.br/handle/icict/7499
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv National Institute of Health
publisher.none.fl_str_mv National Institute of Health
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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