The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism

Detalhes bibliográficos
Autor(a) principal: Rossello, Jessica
Data de Publicação: 2017
Outros Autores: Lima, Analía, Gil, Magdalena, Duarte, Jorge Rodríguez, Correa, Agustín, Carvalho, Paulo Costa, Kierbel, Arlinet, Durán, Rosario
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26742
Resumo: Institut Pasteur de Montevideo. Unidad de Bioquímica y Proteómica Analítica. Montevideo, Uruguay. / Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
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spelling Rossello, JessicaLima, AnalíaGil, MagdalenaDuarte, Jorge RodríguezCorrea, AgustínCarvalho, Paulo CostaKierbel, ArlinetDurán, Rosario2018-06-05T19:09:03Z2018-06-05T19:09:03Z2017ROSSELLO, Jessica et al. The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism. Scientific Reports, v.7, n. 10281, p. 1-14, 2017.2045-2322https://www.arca.fiocruz.br/handle/icict/2674210.1038/s41598-017-09926-3porNature Publishing GroupPseudomonas aeruginosaParasitologyProteomicsParasitologíaProteómicaParasitologiaProteômicaThe EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstitut Pasteur de Montevideo. Unidad de Bioquímica y Proteómica Analítica. Montevideo, Uruguay. / Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.Institut Pasteur de Montevideo. Unidad de Bioquímica y Proteómica Analítica. Montevideo, Uruguay. / Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.Institut Pasteur de Montevideo. Unidad de Bioquímica y Proteómica Analítica. Montevideo, Uruguay. / Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. / Institut Pasteur. Unit of Dynamics of Host-Pathogen Interactions. Paris, France.Institut Pasteur de Montevideo. Unidad de Bioquímica y Proteómica Analítica. Montevideo, Uruguay. / Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.Institut Pasteur de Montevideo. Unidad de Proteínas Recombinantes. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Universidad Nacional de San Martín. Consejo Nacional de Investigaciones Científicas y Técnicas (UNSAM-CONICET). Instituto de Investigaciones Biotecnológicas Dr. Rodolfo A. Ugalde (IIB-INTECH). San Martín, Buenos Aires, Argentina.Institut Pasteur de Montevideo. Unidad de Bioquímica y Proteómica Analítica. Montevideo, Uruguay. / Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.The second messenger c-di-GMP regulates the switch between motile and sessile bacterial lifestyles. A general feature of c-di-GMP metabolism is the presence of a surprisingly large number of genes coding for diguanylate cyclases and phosphodiesterases, the enzymes responsible for its synthesis and degradation respectively. However, the physiological relevance of this apparent redundancy is not clear, emphasizing the need for investigating the functions of each of these enzymes. Here we focused on the phosphodiesterase PA2133 from Pseudomonas aeruginosa, an important opportunistic pathogen. We phenotypically characterized P. aeruginosa strain K overexpressing PA2133 or its inactive mutant. We showed that biofilm formation and motility are severely impaired by overexpression of PA2133. Our quantitative proteomic approach applied to the membrane and exoprotein fractions revealed that proteins involved in three processes were mostly affected: flagellar motility, type III secretion system and chemotaxis. While inhibition of biofilm formation can be ascribed to the phosphodiesterase activity of PA2133, down-regulation of flagellar, chemotaxis, and type III secretion system proteins is independent of this enzymatic activity. Based on these unexpected effects of PA2133, we propose to rename this gene product FcsR, for Flagellar, chemotaxis and type III secretion system Regulator.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/26742/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALCarvalhoTheEAL.pdfCarvalhoTheEAL.pdfapplication/pdf2414066https://www.arca.fiocruz.br/bitstream/icict/26742/2/CarvalhoTheEAL.pdfe83b3fb958bcb605e53cde4368e8ba71MD52TEXTCarvalhoTheEAL.pdf.txtCarvalhoTheEAL.pdf.txtExtracted texttext/plain73089https://www.arca.fiocruz.br/bitstream/icict/26742/3/CarvalhoTheEAL.pdf.txt4c652f810afd8950e16cd0ce4e317587MD53icict/267422018-08-15 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dc.title.pt_BR.fl_str_mv The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism
title The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism
spellingShingle The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism
Rossello, Jessica
Pseudomonas aeruginosa
Parasitology
Proteomics
Parasitología
Proteómica
Parasitologia
Proteômica
title_short The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism
title_full The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism
title_fullStr The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism
title_full_unstemmed The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism
title_sort The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism
author Rossello, Jessica
author_facet Rossello, Jessica
Lima, Analía
Gil, Magdalena
Duarte, Jorge Rodríguez
Correa, Agustín
Carvalho, Paulo Costa
Kierbel, Arlinet
Durán, Rosario
author_role author
author2 Lima, Analía
Gil, Magdalena
Duarte, Jorge Rodríguez
Correa, Agustín
Carvalho, Paulo Costa
Kierbel, Arlinet
Durán, Rosario
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rossello, Jessica
Lima, Analía
Gil, Magdalena
Duarte, Jorge Rodríguez
Correa, Agustín
Carvalho, Paulo Costa
Kierbel, Arlinet
Durán, Rosario
dc.subject.other.pt_BR.fl_str_mv Pseudomonas aeruginosa
topic Pseudomonas aeruginosa
Parasitology
Proteomics
Parasitología
Proteómica
Parasitologia
Proteômica
dc.subject.en.pt_BR.fl_str_mv Parasitology
Proteomics
dc.subject.es.pt_BR.fl_str_mv Parasitología
Proteómica
dc.subject.decs.pt_BR.fl_str_mv Parasitologia
Proteômica
description Institut Pasteur de Montevideo. Unidad de Bioquímica y Proteómica Analítica. Montevideo, Uruguay. / Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-06-05T19:09:03Z
dc.date.available.fl_str_mv 2018-06-05T19:09:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROSSELLO, Jessica et al. The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism. Scientific Reports, v.7, n. 10281, p. 1-14, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26742
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-017-09926-3
identifier_str_mv ROSSELLO, Jessica et al. The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism. Scientific Reports, v.7, n. 10281, p. 1-14, 2017.
2045-2322
10.1038/s41598-017-09926-3
url https://www.arca.fiocruz.br/handle/icict/26742
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/26742/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/26742/2/CarvalhoTheEAL.pdf
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