In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract

Detalhes bibliográficos
Autor(a) principal: Daltro, Sérgio Ricardo Teixeira
Data de Publicação: 2020
Outros Autores: Santos, Ivanilson Pimenta, Barros, Paula Ladeia, Moreira, Diogo Rodrigo Magalhães, Tomassini, Therezinha Coelho Barbosa, Ribeiro, Ivone Maria, Santos, Ricardo Ribeiro dos, Meira, Cássio Santana, Soares, Milena Botelho Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1055/a-1237-4268
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45557
Resumo: CNPq (grant number 562655/ 2010-7), PRONEX (grant number 0002/2014) and FAPESB (grant number 0042/2013)
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spelling Daltro, Sérgio Ricardo TeixeiraSantos, Ivanilson PimentaBarros, Paula LadeiaMoreira, Diogo Rodrigo MagalhãesTomassini, Therezinha Coelho BarbosaRibeiro, Ivone MariaSantos, Ricardo Ribeiro dosMeira, Cássio SantanaSoares, Milena Botelho Pereira2021-01-11T12:57:24Z2021-01-11T12:57:24Z2020DALTRO, Sérgio Ricardo Teixeira et al. In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract. Planta Médica, 2020.0032-0943https://www.arca.fiocruz.br/handle/icict/4555710.1055/a-1237-4268CNPq (grant number 562655/ 2010-7), PRONEX (grant number 0002/2014) and FAPESB (grant number 0042/2013)Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Química de Produtos Naturais-PN2- Extração, Isolamento e Purificação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Química de Produtos Naturais-PN2- Extração, Isolamento e Purificação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.The need for new immunomodulatory drugs is due to the side effects associated with the prolonged use of the currently used immunomodulatory drugs. In this context, the present work aimed to investigate the immunomodulatory effect of an ethanolic concentrated extract from Physalis angulata. The cytotoxicity of samples was determined using peritoneal macrophages though the Alamar Blue assay. The immunomodulatory activity of the ethanolic extract from P. angulata on activated macrophages was determined by measurement of nitrite and cytokine production. The immunosuppressive effects of the ethanolic extract from P. angulata was evaluated on lymphocyte proliferation and cytokine production. The effects of the extract on cell cycle progression and cell death on lymphocytes were evaluated by flow cytometry. Lastly, the ethanolic extract from P. angulata was tested in vivo in toxicological tests and in models of peritonitis and delayed-type hypersensitivity response. The ethanolic extract from P. angulata decreased nitrite, interleukin-6, interleukin-12, and TNF-α production by activated macrophages without affecting the cell viability. In addition, the ethanolic extract from P. angulata inhibited lymphoproliferation and the secretion of interleukin- 2, interleukin-6, and IFN-γ, and increased interleukin-4 secretion by activated splenocytes. Flow cytometry analysis in lymphocyte cultures showed that treatment with the ethanolic extract from P. angulata induces cell cycle arrest in the G1 phase followed by cell death by apoptosis. Moreover, mice treated with the extract from P. angulata at 100 or 200mg/kg did not show signs of toxicity or alterations in serum components. Finally, the ethanolic extract from P. angulata significantly reduced neutrophil migration and reduced paw edema in bovine serum albumin-induced the delayed-type hypersensitivity responsemodel. Our results demonstrate the potential of the ethanolic extract of P. angulata as an alternative for the treatment of immune-inflammatory diseases.engThieme GruppeFunção linfocitáriaPhysalis angulataSolanaceaeAtivação de macrófagoslymphocyte function,Physalis angulataSolanaceaeMacrophage activationIn vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extractinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/45557/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALDaltro Teixeira Ricardo, Sergio In vitro and In vivo....pdfDaltro Teixeira Ricardo, Sergio In vitro and In 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dc.title.pt_BR.fl_str_mv In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract
title In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract
spellingShingle In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract
Daltro, Sérgio Ricardo Teixeira
Função linfocitária
Physalis angulata
Solanaceae
Ativação de macrófagos
lymphocyte function,
Physalis angulata
Solanaceae
Macrophage activation
title_short In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract
title_full In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract
title_fullStr In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract
title_full_unstemmed In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract
title_sort In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract
author Daltro, Sérgio Ricardo Teixeira
author_facet Daltro, Sérgio Ricardo Teixeira
Santos, Ivanilson Pimenta
Barros, Paula Ladeia
Moreira, Diogo Rodrigo Magalhães
Tomassini, Therezinha Coelho Barbosa
Ribeiro, Ivone Maria
Santos, Ricardo Ribeiro dos
Meira, Cássio Santana
Soares, Milena Botelho Pereira
author_role author
author2 Santos, Ivanilson Pimenta
Barros, Paula Ladeia
Moreira, Diogo Rodrigo Magalhães
Tomassini, Therezinha Coelho Barbosa
Ribeiro, Ivone Maria
Santos, Ricardo Ribeiro dos
Meira, Cássio Santana
Soares, Milena Botelho Pereira
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Daltro, Sérgio Ricardo Teixeira
Santos, Ivanilson Pimenta
Barros, Paula Ladeia
Moreira, Diogo Rodrigo Magalhães
Tomassini, Therezinha Coelho Barbosa
Ribeiro, Ivone Maria
Santos, Ricardo Ribeiro dos
Meira, Cássio Santana
Soares, Milena Botelho Pereira
dc.subject.other.pt_BR.fl_str_mv Função linfocitária
Physalis angulata
Solanaceae
Ativação de macrófagos
topic Função linfocitária
Physalis angulata
Solanaceae
Ativação de macrófagos
lymphocyte function,
Physalis angulata
Solanaceae
Macrophage activation
dc.subject.en.pt_BR.fl_str_mv lymphocyte function,
Physalis angulata
Solanaceae
Macrophage activation
description CNPq (grant number 562655/ 2010-7), PRONEX (grant number 0002/2014) and FAPESB (grant number 0042/2013)
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-01-11T12:57:24Z
dc.date.available.fl_str_mv 2021-01-11T12:57:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv DALTRO, Sérgio Ricardo Teixeira et al. In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract. Planta Médica, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45557
dc.identifier.issn.pt_BR.fl_str_mv 0032-0943
dc.identifier.doi.none.fl_str_mv 10.1055/a-1237-4268
identifier_str_mv DALTRO, Sérgio Ricardo Teixeira et al. In vitro and In Vivo Immunomodulatory Activity of Physalis angulata Concentrated Ethanolic Extract. Planta Médica, 2020.
0032-0943
10.1055/a-1237-4268
url https://www.arca.fiocruz.br/handle/icict/45557
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Thieme Gruppe
publisher.none.fl_str_mv Thieme Gruppe
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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