Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus chaetomium thermophilum—sustainable nanotechnology

Detalhes bibliográficos
Autor(a) principal: Alves, Mariana Fuinhas
Data de Publicação: 2022
Outros Autores: Paschoal, Ariane Caroline Campos, Klimeck, Tabata D’Maiella Freitas, Kuligovski, Crisciele, Marcon, Bruna Hilzendeger, Aguiar, Alessandra Melo de, Murray, Patrick G.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54681
Resumo: Shannon Applied Biotechnology Centre. Department of Applied Science. Faculty of Applied Sciences and Technology. Moylish Campus. Technological University of the Shannon. Midlands Midwest, Moylish, Ireland.
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spelling Alves, Mariana FuinhasPaschoal, Ariane Caroline CamposKlimeck, Tabata D’Maiella FreitasKuligovski, CriscieleMarcon, Bruna HilzendegerAguiar, Alessandra Melo deMurray, Patrick G.2022-08-17T15:46:34Z2022-08-17T15:46:34Z2022ALVES, Mariana Fuinhas et al. Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus Chaetomium thermophilum—Sustainable Nanotechnology. Journal of Fungi, v. 8, n. 605, p. 1–15, 2022.2309-608Xhttps://www.arca.fiocruz.br/handle/icict/5468110.3390/jof8060605porMDPIChaetomium thermophilumNanopartículas de prataProtein BiosynthesisToxicityFungiMetal NanoparticlesBiosíntesis de ProteínasToxicidadHongosNanopartículas del MetalBiossíntese de ProteínasChaetomiumToxicidadeFungosNanopartículas MetálicasBiological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus chaetomium thermophilum—sustainable nanotechnologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleShannon Applied Biotechnology Centre. Department of Applied Science. Faculty of Applied Sciences and Technology. Moylish Campus. Technological University of the Shannon. Midlands Midwest, Moylish, Ireland.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Plataforma de Microscopia. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Plataforma de Microscopia. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Bioensaios com Métodos Alternativos em Citotoxicidade. Curitiba, PR, Brasil.Shannon Applied Biotechnology Centre. Department of Applied Science. Faculty of Applied Sciences and Technology. Moylish Campus. Technological University of the Shannon. Midlands Midwest, Moylish, Ireland.Fungal biotechnology research has rapidly increased as a result of the growing awareness of sustainable development and the pressing need to explore eco-friendly options. In the nanotechnology field, silver nanoparticles (AgNPs) are currently being studied for application in cancer therapy, tumour detection, drug delivery, and elsewhere. Therefore, synthesising nanoparticles (NPs) with low toxicity has become essential in the biomedical area. The fungus Chaetomium thermophilum (C. thermophilum) was here investigated—to the best of our knowledge, for the first time—for application in the production of AgNPs. Transmission electronic microscopy (TEM) images demonstrated a spherical AgNP shape, with an average size of 8.93 nm. Energy-dispersive X-ray spectrometry (EDX) confirmed the presence of elemental silver. A neutral red uptake (NRU) test evaluated the cytotoxicity of the AgNPs at different inhibitory concentrations (ICs). A half-maximal concentration (IC50 = 119.69 µg/mL) was used to predict a half-maximal lethal dose (LD50 = 624.31 mg/kg), indicating a Global Harmonized System of Classification and Labelling of Chemicals (GHS) acute toxicity estimate (ATE) classification category of 4. The fungus extract showed a non-toxic profile at the IC tested. Additionally, the interaction between the AgNPs and the Balb/c 3T3 NIH cells at an ultrastructural level resulted in preserved cells structures at non-toxic concentrations (IC20 = 91.77 µg/mL), demonstrating their potential as sustainable substitutes for physical and chemically made AgNPs. Nonetheless, at the IC50, the cytoplasm of the cells was damaged and mitochondrial morphological alteration was evident. This fact highlights the fact that dose-dependent phenomena are involved, as well as emphasising the importance of investigating NPs’ effects on mitochondria, as disruption to this organelle can impact health.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/54681/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALjof-08-00605ok.pdfjof-08-00605ok.pdfapplication/pdf4343220https://www.arca.fiocruz.br/bitstream/icict/54681/2/jof-08-00605ok.pdfaeac7ba7aa412847bbd3da92b8a61118MD52icict/546812022-08-17 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dc.title.pt_BR.fl_str_mv Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus chaetomium thermophilum—sustainable nanotechnology
title Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus chaetomium thermophilum—sustainable nanotechnology
spellingShingle Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus chaetomium thermophilum—sustainable nanotechnology
Alves, Mariana Fuinhas
Chaetomium thermophilum
Nanopartículas de prata
Protein Biosynthesis
Toxicity
Fungi
Metal Nanoparticles
Biosíntesis de Proteínas
Toxicidad
Hongos
Nanopartículas del Metal
Biossíntese de Proteínas
Chaetomium
Toxicidade
Fungos
Nanopartículas Metálicas
title_short Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus chaetomium thermophilum—sustainable nanotechnology
title_full Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus chaetomium thermophilum—sustainable nanotechnology
title_fullStr Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus chaetomium thermophilum—sustainable nanotechnology
title_full_unstemmed Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus chaetomium thermophilum—sustainable nanotechnology
title_sort Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus chaetomium thermophilum—sustainable nanotechnology
author Alves, Mariana Fuinhas
author_facet Alves, Mariana Fuinhas
Paschoal, Ariane Caroline Campos
Klimeck, Tabata D’Maiella Freitas
Kuligovski, Crisciele
Marcon, Bruna Hilzendeger
Aguiar, Alessandra Melo de
Murray, Patrick G.
author_role author
author2 Paschoal, Ariane Caroline Campos
Klimeck, Tabata D’Maiella Freitas
Kuligovski, Crisciele
Marcon, Bruna Hilzendeger
Aguiar, Alessandra Melo de
Murray, Patrick G.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alves, Mariana Fuinhas
Paschoal, Ariane Caroline Campos
Klimeck, Tabata D’Maiella Freitas
Kuligovski, Crisciele
Marcon, Bruna Hilzendeger
Aguiar, Alessandra Melo de
Murray, Patrick G.
dc.subject.other.pt_BR.fl_str_mv Chaetomium thermophilum
Nanopartículas de prata
topic Chaetomium thermophilum
Nanopartículas de prata
Protein Biosynthesis
Toxicity
Fungi
Metal Nanoparticles
Biosíntesis de Proteínas
Toxicidad
Hongos
Nanopartículas del Metal
Biossíntese de Proteínas
Chaetomium
Toxicidade
Fungos
Nanopartículas Metálicas
dc.subject.en.pt_BR.fl_str_mv Protein Biosynthesis
Toxicity
Fungi
Metal Nanoparticles
dc.subject.es.pt_BR.fl_str_mv Biosíntesis de Proteínas
Toxicidad
Hongos
Nanopartículas del Metal
dc.subject.decs.pt_BR.fl_str_mv Biossíntese de Proteínas
Chaetomium
Toxicidade
Fungos
Nanopartículas Metálicas
description Shannon Applied Biotechnology Centre. Department of Applied Science. Faculty of Applied Sciences and Technology. Moylish Campus. Technological University of the Shannon. Midlands Midwest, Moylish, Ireland.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-08-17T15:46:34Z
dc.date.available.fl_str_mv 2022-08-17T15:46:34Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ALVES, Mariana Fuinhas et al. Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus Chaetomium thermophilum—Sustainable Nanotechnology. Journal of Fungi, v. 8, n. 605, p. 1–15, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54681
dc.identifier.issn.pt_BR.fl_str_mv 2309-608X
dc.identifier.doi.none.fl_str_mv 10.3390/jof8060605
identifier_str_mv ALVES, Mariana Fuinhas et al. Biological synthesis of low cytotoxicity silver nanoparticles (AgNPs) by the fungus Chaetomium thermophilum—Sustainable Nanotechnology. Journal of Fungi, v. 8, n. 605, p. 1–15, 2022.
2309-608X
10.3390/jof8060605
url https://www.arca.fiocruz.br/handle/icict/54681
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/54681/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/54681/2/jof-08-00605ok.pdf
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