Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40468 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Treinamento em Malária Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, SVS & Fiocruz. Rio de Janeiro, RJ, Brazil. |
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Almeida-de-Oliveira, Natália KetrinLima-Cury, LidianeAbreu-Fernandes, Rebecca deRosa Lavigne, Aline dePina-Costa, Anielle deSouza Perce-da-Silva, Daiana deCatanho, MarcosBrasil, PatríciaDaniel-Ribeiro, Cláudio TadeuFerreira-da-Cruz, Maria de Fátima2020-03-24T00:40:21Z2020-03-24T00:40:21Z2020ALMEIDA-DE-OLIVEIRA, Natália Ketrin et al. Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection. Malaria Journal, v. 19, n. 1, p. 1-9, 2020.1475-2875https://www.arca.fiocruz.br/handle/icict/4046810.1186/s12936-020-03159-yengBMCExtensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Treinamento em Malária Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, SVS & Fiocruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Treinamento em Malária Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, SVS & Fiocruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Treinamento em Malária Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, SVS & Fiocruz, Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Treinamento em Malária Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, SVS & Fiocruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Treinamento em Malária Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, SVS & Fiocruz. Rio de Janeiro, RJ, Brazil / Centro Universitário Serra dos Órgãos. Teresópolis, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Treinamento em Malária Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, SVS & Fiocruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Treinamento em Malária Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, SVS & Fiocruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Treinamento em Malária Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, SVS & Fiocruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Treinamento em Malária Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, SVS & Fiocruz. Rio de Janeiro, RJ, Brazil.Background: Plasmodium vivax is the most widespread human malaria parasite outside Africa and is the predominant parasite in the Americas. Increasing reports of P. vivax disease severity, together with the emergence of drugresistant strains, underscore the urgency of the development of vaccines against P. vivax. Polymorphisms on DBP-IIgene could act as an immune evasion mechanism and, consequently, limited the vaccine efcacy. This study aimed to investigate the pvdbp-II genetic diversity in two Brazilian regions with diferent epidemiological patterns: the unstable transmission area in the Atlantic Forest (AF) of Rio de Janeiro and; the fxed malaria-endemic area in Brazilian Amazon (BA). Methods: 216 Brazilian P. vivax infected blood samples, diagnosed by microscopic examination and PCR, were investigated. The region fanking pvdbp-II was amplifed by PCR and sequenced. Genetic polymorphisms of pvdbp-II were estimated based on the number of segregating sites and nucleotide and haplotype diversities; the degree of diferentiation between-regions was evaluated applying Wright’s statistics. Natural selection was calculated using the rate of nonsynonymous per synonymous substitutions with the Z-test, and the evolutionary distance was estimated based on the reconstructed tree. Results: 79 samples from AF and 137 from BA were successfully sequenced. The analyses showed 28 polymorphic sites distributed in 21 codons, with only 5% of the samples Salvador 1 type. The highest rates of polymorphic sites were found in B- and T cell epitopes. Unexpectedly, the nucleotide diversity in pvdbp-II was higher in AF (0.01) than in BA (0.008). Among the 28 SNPs detected, 18 are shared between P. vivax isolates from AF and BA regions, but 8 SNPs were exclusively detected in AF—I322S, K371N, E385Q, E385T, K386T, K411N, I419L and I419R—and 2 (N375D and I419M) arose exclusively in BA. These fndings could suggest the potential of these geographical clusters as population-specifc-signatures that may be useful to track the origin of infections. The sample size should be increased in order to confrm this possibility.DBPGenetic diversityMalariaPlasmodium vivaxPositive selectionSNPinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/40468/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALExtensive Genetic_Patricia_Brasil_etal_INI_2020.pdfExtensive Genetic_Patricia_Brasil_etal_INI_2020.pdfapplication/pdf1629547https://www.arca.fiocruz.br/bitstream/icict/40468/2/Extensive%20Genetic_Patricia_Brasil_etal_INI_2020.pdfb147ad8b01b1a4dd498b8eb6e699ea99MD52TEXTExtensive Genetic_Patricia_Brasil_etal_INI_2020.pdf.txtExtensive Genetic_Patricia_Brasil_etal_INI_2020.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection |
| title |
Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection |
| spellingShingle |
Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection Almeida-de-Oliveira, Natália Ketrin DBP Genetic diversity Malaria Plasmodium vivax Positive selection SNP |
| title_short |
Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection |
| title_full |
Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection |
| title_fullStr |
Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection |
| title_full_unstemmed |
Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection |
| title_sort |
Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection |
| author |
Almeida-de-Oliveira, Natália Ketrin |
| author_facet |
Almeida-de-Oliveira, Natália Ketrin Lima-Cury, Lidiane Abreu-Fernandes, Rebecca de Rosa Lavigne, Aline de Pina-Costa, Anielle de Souza Perce-da-Silva, Daiana de Catanho, Marcos Brasil, Patrícia Daniel-Ribeiro, Cláudio Tadeu Ferreira-da-Cruz, Maria de Fátima |
| author_role |
author |
| author2 |
Lima-Cury, Lidiane Abreu-Fernandes, Rebecca de Rosa Lavigne, Aline de Pina-Costa, Anielle de Souza Perce-da-Silva, Daiana de Catanho, Marcos Brasil, Patrícia Daniel-Ribeiro, Cláudio Tadeu Ferreira-da-Cruz, Maria de Fátima |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Almeida-de-Oliveira, Natália Ketrin Lima-Cury, Lidiane Abreu-Fernandes, Rebecca de Rosa Lavigne, Aline de Pina-Costa, Anielle de Souza Perce-da-Silva, Daiana de Catanho, Marcos Brasil, Patrícia Daniel-Ribeiro, Cláudio Tadeu Ferreira-da-Cruz, Maria de Fátima |
| dc.subject.en.pt_BR.fl_str_mv |
DBP Genetic diversity Malaria Plasmodium vivax Positive selection SNP |
| topic |
DBP Genetic diversity Malaria Plasmodium vivax Positive selection SNP |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Malária. Rio de Janeiro, RJ, Brasil / Centro de Pesquisa, Diagnóstico e Treinamento em Malária Reference Laboratory for Malaria in the Extra-Amazonian Region for the Brazilian Ministry of Health, SVS & Fiocruz. Rio de Janeiro, RJ, Brazil. |
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2020 |
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2020 |
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ALMEIDA-DE-OLIVEIRA, Natália Ketrin et al. Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection. Malaria Journal, v. 19, n. 1, p. 1-9, 2020. |
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https://www.arca.fiocruz.br/handle/icict/40468 |
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1475-2875 |
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10.1186/s12936-020-03159-y |
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ALMEIDA-DE-OLIVEIRA, Natália Ketrin et al. Extensive genetic diversity of Plasmodium vivax dbp-II in Rio de Janeiro Atlantic Forest and Brazilian Amazon Basin: evidence of positive selection. Malaria Journal, v. 19, n. 1, p. 1-9, 2020. 1475-2875 10.1186/s12936-020-03159-y |
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