Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11715 |
Resumo: | Fundação Oswaldo Cruz. Departamento de Primatologia. Centro de Criação de Animais de Laboratório. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. |
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Carvalho, Lilian G. deMarchevsky, Renato S.Santos, Debora R. L. dosOliveira, Jaqueline M. dePaula, Vanessa S. deLopes, Leilane M.Van der Poel, Wilhelmus H. M.González, Jorge E.Munné, Maria S.Moran, JulioCajaraville, Ana Carolina R. A.Machado, Marcelo PelajoCruz, Oswaldo GonçalvesPinto, Marcelo A.2015-09-21T17:25:27Z2015-09-21T17:25:27Z2013CARVALHO, Lilian G. de; et al. Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis. BMC Infectious Diseases, v.13, n.495, 11p, 2013.https://www.arca.fiocruz.br/handle/icict/1171510.1186/1471-2334-13-495engBioMed CentralInfection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Departamento de Primatologia. Centro de Criação de Animais de Laboratório. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Biomanguinhos. Instituto de Tecnologia em Imunobiológicos. Controle de Neurovirulência. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Departamento de Microbiologia e Imunologia Veterinária. Vírus Veterinários. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Institute of Wageningen University and Research Centre. Central veterinary. Wageningen, The Netherlands.National Institute of Infectious Diseases. National Reference Laboratory in Viral Hepatitis. Buenos Aires, Argentina.National Institute of Infectious Diseases. National Reference Laboratory in Viral Hepatitis. Buenos Aires, Argentina.Dr. Julio Moran laboratories. Ebmatingen, Zurich, Switzerland.Fundação Oswaldo Cruz. Biomanguinhos. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Background: Hepatitis E virus (HEV) has been described as an emerging pathogen in Brazil and seems to be widely disseminated among swine herds. An autochthonous human case of acute hepatitis E was recently reported. To obtain a better understanding of the phenotypic profiles of both human and swine HEV strains, a experimental study was conducted using the animal model, Macaca fascicularis. Methods: Six cynomolgus monkeys (Macaca fascicularis) were inoculated intravenously with swine HEV genotype 3 that was isolated from naturally and experimentally infected pigs in Brazil and the Netherlands. Two other monkeys were inoculated with HEV genotype 3 that was recovered from Brazilian and Argentinean patients with locally acquired acute and fulminant hepatitis E. The haematological, biochemical, and virological parameters of all animals were monitored for 67 days. Results: Subclinical hepatitis was observed in all monkeys after inoculation with HEV genotype 3 that was recovered from the infected swine and human patients. HEV RNA was detected in the serum and/or faeces of 6 out of the 8 cynomolgus monkeys between 5 and 53 days after inoculation. The mild inflammation of liver tissues and elevations of discrete liver enzymes were observed. Seroconversions to anti-HEV IgM and/or IgG were detected in 7 animals. Reactivities to anti-HEV IgA were also detected in the salivary samples of 3 animals. Interestingly, all of the infected monkeys showed severe lymphopenia and a trend toward monocytosis, which coincided with elevations in alanine aminotransferase and antibody titres. Conclusions: The ability of HEV to cross the species barrier was confirmed for both the swine (Brazilian and Dutch) and human (Argentinean) strains, thus reinforcing the zoonotic risk of hepatitis E in South America. Cynomolgus monkeys that were infected with HEV genotype 3 developed subclinical hepatitis that was associated with haematological changes. Haematological approaches should be considered in future studies of HEV infection.Swine HEVHuman HEVLymphopeniaFulminant hepatic failureGenotype 3Macaca fascicularisHepatitis E virus (HEV)Macaca fascicularisGenótipo 3Vírus da Hepatite ELymphopeniainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11715/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALleilane_lopes_etal_IOC_2013.pdfapplication/pdf885524https://www.arca.fiocruz.br/bitstream/icict/11715/2/leilane_lopes_etal_IOC_2013.pdf58174dd00bdf41d17294b9d787da45c4MD52TEXTleilane_lopes_etal_IOC_2013.pdf.txtleilane_lopes_etal_IOC_2013.pdf.txtExtracted texttext/plain55816https://www.arca.fiocruz.br/bitstream/icict/11715/3/leilane_lopes_etal_IOC_2013.pdf.txtf94cc2f48486f594335d3988c707b489MD53icict/117152022-06-24 12:17:45.466oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:45Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis |
| title |
Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis |
| spellingShingle |
Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis Carvalho, Lilian G. de Swine HEV Human HEV Lymphopenia Fulminant hepatic failure Genotype 3 Macaca fascicularis Hepatitis E virus (HEV) Macaca fascicularis Genótipo 3 Vírus da Hepatite E Lymphopenia |
| title_short |
Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis |
| title_full |
Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis |
| title_fullStr |
Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis |
| title_full_unstemmed |
Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis |
| title_sort |
Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis |
| author |
Carvalho, Lilian G. de |
| author_facet |
Carvalho, Lilian G. de Marchevsky, Renato S. Santos, Debora R. L. dos Oliveira, Jaqueline M. de Paula, Vanessa S. de Lopes, Leilane M. Van der Poel, Wilhelmus H. M. González, Jorge E. Munné, Maria S. Moran, Julio Cajaraville, Ana Carolina R. A. Machado, Marcelo Pelajo Cruz, Oswaldo Gonçalves Pinto, Marcelo A. |
| author_role |
author |
| author2 |
Marchevsky, Renato S. Santos, Debora R. L. dos Oliveira, Jaqueline M. de Paula, Vanessa S. de Lopes, Leilane M. Van der Poel, Wilhelmus H. M. González, Jorge E. Munné, Maria S. Moran, Julio Cajaraville, Ana Carolina R. A. Machado, Marcelo Pelajo Cruz, Oswaldo Gonçalves Pinto, Marcelo A. |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Carvalho, Lilian G. de Marchevsky, Renato S. Santos, Debora R. L. dos Oliveira, Jaqueline M. de Paula, Vanessa S. de Lopes, Leilane M. Van der Poel, Wilhelmus H. M. González, Jorge E. Munné, Maria S. Moran, Julio Cajaraville, Ana Carolina R. A. Machado, Marcelo Pelajo Cruz, Oswaldo Gonçalves Pinto, Marcelo A. |
| dc.subject.en.pt_BR.fl_str_mv |
Swine HEV Human HEV Lymphopenia Fulminant hepatic failure Genotype 3 Macaca fascicularis Hepatitis E virus (HEV) |
| topic |
Swine HEV Human HEV Lymphopenia Fulminant hepatic failure Genotype 3 Macaca fascicularis Hepatitis E virus (HEV) Macaca fascicularis Genótipo 3 Vírus da Hepatite E Lymphopenia |
| dc.subject.decs.pt_BR.fl_str_mv |
Macaca fascicularis Genótipo 3 Vírus da Hepatite E Lymphopenia |
| description |
Fundação Oswaldo Cruz. Departamento de Primatologia. Centro de Criação de Animais de Laboratório. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. |
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2013 |
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2013 |
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2015-09-21T17:25:27Z |
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2015-09-21T17:25:27Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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CARVALHO, Lilian G. de; et al. Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis. BMC Infectious Diseases, v.13, n.495, 11p, 2013. |
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https://www.arca.fiocruz.br/handle/icict/11715 |
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10.1186/1471-2334-13-495 |
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CARVALHO, Lilian G. de; et al. Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis. BMC Infectious Diseases, v.13, n.495, 11p, 2013. 10.1186/1471-2334-13-495 |
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BioMed Central |
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