Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/45493 |
Resumo: | FIOCRUZ (VMB), FAPESP Grant fellowships (2015/ 15948-5, 2016/10145-4, 2016/10345-3, 2016/11116-8 and 2017/22888-4) (FMO) and by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - Finance Code 001. AAB, CIO and VMB are senior scientist fellows at the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). JRS is supported by a scholarship from CAPES. PSSM holds a fellowship from Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). ALM has a fellowship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). |
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Silva, Jéssica RebouçasTadini, Maraine CatarinaNunes, Danielle DevequiMansur, Ana LuízaMattos, Paulo Sérgio de Morais da SilveiraOliveira, Camila Indiani deFormiga, Fabio RochaBerretta, Andresa A.Oliveira, Franciane MarqueleBorges, Valeria de Matos2021-01-04T14:34:31Z2021-01-04T14:34:31Z2020SILVA, Jéssica Rebouças et al. Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection. International Journal of Nanomedicine, p. 8659-8672, 2020.1178-2013https://www.arca.fiocruz.br/handle/icict/4549310.2147/IJN.S262642FIOCRUZ (VMB), FAPESP Grant fellowships (2015/ 15948-5, 2016/10145-4, 2016/10345-3, 2016/11116-8 and 2017/22888-4) (FMO) and by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - Finance Code 001. AAB, CIO and VMB are senior scientist fellows at the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). JRS is supported by a scholarship from CAPES. PSSM holds a fellowship from Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). ALM has a fellowship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Medicine of Bahia. Postgraduate Program in Pathology. Salvador, BA, Brazil.Eleve Science Research and Development. Ribeirão Preto, SP, Brazil / University of São Paulo. Faculty of Philosophy, Sciences and Letters of Ribeirão Preto. Department of Chemistry. Ribeirão Preto, SP, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Medicine of Bahia. Postgraduate Program in Pathology. Salvador, BA, Brazil. SENAI Institute of Innovation in Advanced Health Systems. Laboratory of Pharmaceutical Formulations, Salvador, BA, Brazil.Eleve Science Research and Development. Ribeirão Preto, SP, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Medicine of Bahia. Postgraduate Program in Pathology. Salvador, BA, Brazil.Eleve Science Research and Development. Ribeirão Preto, SP, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Vector- Doenças Infecciosas Transmitidas. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil / University of Pernambuco. Postgraduate Program in Applied Cellular and Molecular Biology. Recife, PE, Brazil.Apis Flora Industrial e Comercial Ltda. Laboratory of Research, Development and Innovation. Ribeirão Preto, SP, Brazil.Eleve Science Research and Development. Ribeirão Preto, SP, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Inflamação e Biomarcadores. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Medicine of Bahia. Postgraduate Program in Pathology. Salvador, BA, Brazil.Leishmaniasis is a neglected disease, and the current therapeutic arsenal for its treatment is seriously limited by high cost and toxicity. Nanostructured lipid carriers (NLCs) represent a promising approach due to high drug loading capacity, controlled drug release profiles and superior stability. Here, we explore the efficacy of a unique pH-sensitive amphotericin B-loaded NLC (AmB-NLC) in Leishmania braziliensis infection in vitro and in vivo. Methods and Results: AmB-NLC was assessed by dynamic light scattering and atomic force microscopy assays. The carrier showed a spherical shape with a nanometric size of 242.0 ± 18.3 nm. Zeta potential was suggestive of high carrier stability (−42.5 ± 1.5 mV), and the NLC showed ~99% drug encapsulation efficiency (EE%). In biological assays, AmB-NLC presented a similar IC50 as free AmB and conventional AmB deoxycholate (AmB-D) (11.7 ± 1.73; 5.3 ± 0.55 and 13 ± 0.57 ng/mL, respectively), while also presenting higher selectivity index and lower toxicity to host cells, with no observed production of nitric oxide or TNF-α by in vitro assay. Confocal microscopy revealed the rapid uptake of AmBNLC by infected macrophages after 1h, which, in association with more rapid disruption of AmB-NLC at acidic pH levels, may directly affect intracellular parasites. Leishmanicidal effects were evaluated in vivo in BALB/c mice infected in the ear dermis with L. braziliensis and treated with a pentavalent antimonial (Sb5+), liposomal AmB (AmB-L) or AmB-NLC. After 6 weeks of infection, AmB-NLC treatment resulted in smaller ear lesion size in all treated mice, indicating the efficacy of the novel formulation. Conclusion: Here, we preliminarily demonstrate the effectiveness of an innovative and cost-effective AmB-NLC formulation in promoting the killing of intracellular L. braziliensis. This novel carrier system could be a promising alternative for the future treatment of cutaneous leishmaniasis.engAmerican Society for NanomedicineLeishmanioseDoença negligenciadaNanopartículasAdministração de drogasLeishmaniasisNeglected diseaseNanoparticlesDrug deliveryEvaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/45493/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALSilva Jessica Reboucas Evaluation....pdfSilva Jessica Reboucas 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| dc.title.pt_BR.fl_str_mv |
Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection |
| title |
Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection |
| spellingShingle |
Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection Silva, Jéssica Rebouças Leishmaniose Doença negligenciada Nanopartículas Administração de drogas Leishmaniasis Neglected disease Nanoparticles Drug delivery |
| title_short |
Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection |
| title_full |
Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection |
| title_fullStr |
Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection |
| title_full_unstemmed |
Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection |
| title_sort |
Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection |
| author |
Silva, Jéssica Rebouças |
| author_facet |
Silva, Jéssica Rebouças Tadini, Maraine Catarina Nunes, Danielle Devequi Mansur, Ana Luíza Mattos, Paulo Sérgio de Morais da Silveira Oliveira, Camila Indiani de Formiga, Fabio Rocha Berretta, Andresa A. Oliveira, Franciane Marquele Borges, Valeria de Matos |
| author_role |
author |
| author2 |
Tadini, Maraine Catarina Nunes, Danielle Devequi Mansur, Ana Luíza Mattos, Paulo Sérgio de Morais da Silveira Oliveira, Camila Indiani de Formiga, Fabio Rocha Berretta, Andresa A. Oliveira, Franciane Marquele Borges, Valeria de Matos |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Jéssica Rebouças Tadini, Maraine Catarina Nunes, Danielle Devequi Mansur, Ana Luíza Mattos, Paulo Sérgio de Morais da Silveira Oliveira, Camila Indiani de Formiga, Fabio Rocha Berretta, Andresa A. Oliveira, Franciane Marquele Borges, Valeria de Matos |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose Doença negligenciada Nanopartículas Administração de drogas |
| topic |
Leishmaniose Doença negligenciada Nanopartículas Administração de drogas Leishmaniasis Neglected disease Nanoparticles Drug delivery |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmaniasis Neglected disease Nanoparticles Drug delivery |
| description |
FIOCRUZ (VMB), FAPESP Grant fellowships (2015/ 15948-5, 2016/10145-4, 2016/10345-3, 2016/11116-8 and 2017/22888-4) (FMO) and by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - Finance Code 001. AAB, CIO and VMB are senior scientist fellows at the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). JRS is supported by a scholarship from CAPES. PSSM holds a fellowship from Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). ALM has a fellowship from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). |
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2020 |
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2020 |
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2021-01-04T14:34:31Z |
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2021-01-04T14:34:31Z |
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info:eu-repo/semantics/article |
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SILVA, Jéssica Rebouças et al. Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection. International Journal of Nanomedicine, p. 8659-8672, 2020. |
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https://www.arca.fiocruz.br/handle/icict/45493 |
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1178-2013 |
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10.2147/IJN.S262642 |
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SILVA, Jéssica Rebouças et al. Evaluation of in vitro and in vivo Efficacy of a Novel Amphotericin B-Loaded Nanostructured Lipid Carrier in the Treatment of Leishmania braziliensis Infection. International Journal of Nanomedicine, p. 8659-8672, 2020. 1178-2013 10.2147/IJN.S262642 |
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American Society for Nanomedicine |
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American Society for Nanomedicine |
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