Development and validation of the first SSR markers for Mimosa scabrella Benth

Detalhes bibliográficos
Autor(a) principal: Saiki, F. A.
Data de Publicação: 2017
Outros Autores: Bernardi, A. P., Reis, M. S., Faoro, Helisson, Souza, Emanuel Maltempi de, Pedrosa, Fábio Oliveira, Mantovani, A., Guidolin, A. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20905
Resumo: Universidade do Estado de Santa Catarina. Departamento de Agronomia. Lages, SC, Brasil.
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spelling Saiki, F. A.Bernardi, A. P.Reis, M. S.Faoro, HelissonSouza, Emanuel Maltempi dePedrosa, Fábio OliveiraMantovani, A.Guidolin, A. F.2017-09-04T19:41:23Z2017-09-04T19:41:23Z2017SAIKI, F. A. et al. Development and validation of the first SSR markers for Mimosa scabrella Benth. Genetics and Molecular Research, v. 16, n. 1, p. 1-8, 2017.https://www.arca.fiocruz.br/handle/icict/2090510.4238/gmr160195711676-5680engFUNPEC-RPMimosaSequence AnalysisPolyploidyMicrosatellite RepeatsAnálisis de Secuencia de ADNPoliploidíaRepeticiones de MicrosatéliteAnálise de Sequência de DNAPoliploidiaRepetições de MicrossatélitesDevelopment and validation of the first SSR markers for Mimosa scabrella Benthinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado de Santa Catarina. Departamento de Agronomia. Lages, SC, Brasil.Universidade Federal de Santa Catarina. Departamento de Fitotecnia. Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina. Departamento de Fitotecnia. Florianópolis, SC, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil.Universidade do Estado de Santa Catarina. Departamento de Engenharia Florestal. Lages, SC, Brasil.Universidade do Estado de Santa Catarina. Departamento de Agronomia. Lages, SC, Brasil.Mimosa scabrella Benth., popularly known as ''bracatinga'', is a pioneer and endemic species of Brazil, occurring in Mixed Ombrophilous Forest associated with Brazilian Atlantic Rainforest biomes. It is a fast-growing tree of the Fabaceae family that facilitates the dynamics of ecological succession. SSR development, when there is no genome sequence, is time and labor intensive and there are no molecular markers for M. scabrella. We developed and validated the first microsatellite markers for this tetraploid species, evaluating mother trees and progenies. Using Illumina sequencing, we identified 290 SSR loci and 211 primer pairs. After 31 SSR loci PCR/agarose electrophoresis selection, a subset of 11 primer pairs was synthetized with fluorescence in the forward primer for PCR and capillary electrophoresis validation with leaf DNA of 33 adult and 411 progeny individuals. Polymorphic locus percentage was 36, 4 in 11 loci, 3 chloroplast SSRs, and 1 nuclear SSR. Allele number of polymorphic loci ranged from 2 to 11 alleles considering all sampling. All 11 primer pairs were also tested for cross-species amplification for five Fabaceae-Mimosoideae species, ranging from 2 loci transferred to Calliandra tweedii Benth. and all 11 loci transferred to Mimosa taimbensis Burkart. The assessed and validated SSR markers for M. scabrella are suitable and useful for analysis and population genetic studies.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/20905/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALsaikidevelopement.pdfsaikidevelopement.pdfapplication/pdf234606https://www.arca.fiocruz.br/bitstream/icict/20905/2/saikidevelopement.pdf1a69a69fa4642db506fc21e14754a72eMD52TEXTsaikidevelopement.pdf.txtsaikidevelopement.pdf.txtExtracted texttext/plain27971https://www.arca.fiocruz.br/bitstream/icict/20905/3/saikidevelopement.pdf.txtb1916a267b3106480239e01412cef2f5MD53icict/209052019-11-23 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dc.title.pt_BR.fl_str_mv Development and validation of the first SSR markers for Mimosa scabrella Benth
title Development and validation of the first SSR markers for Mimosa scabrella Benth
spellingShingle Development and validation of the first SSR markers for Mimosa scabrella Benth
Saiki, F. A.
Mimosa
Sequence Analysis
Polyploidy
Microsatellite Repeats
Análisis de Secuencia de ADN
Poliploidía
Repeticiones de Microsatélite
Análise de Sequência de DNA
Poliploidia
Repetições de Microssatélites
title_short Development and validation of the first SSR markers for Mimosa scabrella Benth
title_full Development and validation of the first SSR markers for Mimosa scabrella Benth
title_fullStr Development and validation of the first SSR markers for Mimosa scabrella Benth
title_full_unstemmed Development and validation of the first SSR markers for Mimosa scabrella Benth
title_sort Development and validation of the first SSR markers for Mimosa scabrella Benth
author Saiki, F. A.
author_facet Saiki, F. A.
Bernardi, A. P.
Reis, M. S.
Faoro, Helisson
Souza, Emanuel Maltempi de
Pedrosa, Fábio Oliveira
Mantovani, A.
Guidolin, A. F.
author_role author
author2 Bernardi, A. P.
Reis, M. S.
Faoro, Helisson
Souza, Emanuel Maltempi de
Pedrosa, Fábio Oliveira
Mantovani, A.
Guidolin, A. F.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Saiki, F. A.
Bernardi, A. P.
Reis, M. S.
Faoro, Helisson
Souza, Emanuel Maltempi de
Pedrosa, Fábio Oliveira
Mantovani, A.
Guidolin, A. F.
dc.subject.other.pt_BR.fl_str_mv Mimosa
topic Mimosa
Sequence Analysis
Polyploidy
Microsatellite Repeats
Análisis de Secuencia de ADN
Poliploidía
Repeticiones de Microsatélite
Análise de Sequência de DNA
Poliploidia
Repetições de Microssatélites
dc.subject.en.pt_BR.fl_str_mv Sequence Analysis
Polyploidy
Microsatellite Repeats
dc.subject.es.pt_BR.fl_str_mv Análisis de Secuencia de ADN
Poliploidía
Repeticiones de Microsatélite
dc.subject.decs.pt_BR.fl_str_mv Análise de Sequência de DNA
Poliploidia
Repetições de Microssatélites
description Universidade do Estado de Santa Catarina. Departamento de Agronomia. Lages, SC, Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-09-04T19:41:23Z
dc.date.available.fl_str_mv 2017-09-04T19:41:23Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SAIKI, F. A. et al. Development and validation of the first SSR markers for Mimosa scabrella Benth. Genetics and Molecular Research, v. 16, n. 1, p. 1-8, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20905
dc.identifier.doi.none.fl_str_mv 10.4238/gmr16019571
dc.identifier.eissn.none.fl_str_mv 1676-5680
identifier_str_mv SAIKI, F. A. et al. Development and validation of the first SSR markers for Mimosa scabrella Benth. Genetics and Molecular Research, v. 16, n. 1, p. 1-8, 2017.
10.4238/gmr16019571
1676-5680
url https://www.arca.fiocruz.br/handle/icict/20905
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv FUNPEC-RP
publisher.none.fl_str_mv FUNPEC-RP
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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