Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/26134 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. |
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Sardella, Isabela GamaSingh, MahavirKumpfer, SusanneHeringer, Rafael RibeiroSaad, Maria Helena FéresSohler, Marzia Puccioni2018-04-26T14:53:33Z2018-04-26T14:53:33Z2010SARDELLA, Isabela Gama; et al. Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients. Mem Inst Oswaldo Cruz, v.105, n.5, p. 722-728, Aug. 2010.0074-0276https://www.arca.fiocruz.br/handle/icict/261341678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzTuberculoseLíquido CefalorraquidianoEnsaio de Imunoadsorção EnzimáticaMycobacterium tuberculosisResposta imunetuberculosiscerebrospinal fluidELISAmeningoencephalitis tuberculosisMycobacterium tuberculosisimmune responseEvaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Lionex Diagnostics and Therapeutics. Braunschweig, Germany.Lionex Diagnostics and Therapeutics. Braunschweig, Germany.Universidade Federal do Rio de Janeiro. Laboratório Neurolife. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Laboratório Neurolife. Rio de Janeiro, RJ, Brasil.To evaluate commercial Lionex TB together with four antigens of Mycobacterium tuberculosis (MPT-64, MT10.3, 16 kDa and 38 kDa) for IgG and IgA cerebrospinal fluid (CSF) detection in the diagnosis of tuberculosis meningitis (TBM) with CSF negative acid-fast bacilli staining, 19 cases of TBM, 64 cases of other infectious meningoencephalitis and 73 cases of other neurological disorders were tested by enzyme linked immunosorbent assay. IgA-MPT-64 and IgG Lionex showed the highest sensitivities, specificities, positive predictive value and negative predictive value (63.2%, 47.4%; 95%, 93.7%; 40%, 98% and 28.4%, 97.1%, respectively). However, while grey zone was 12.7% and 6%, respectively, lowering sensitivity but maintains high specificity (≥ 95%). High protein concentration in CSF was associated with antibody positivity CSF/HIV+ which did not influence the sensitivity of both tests. To our knowledge, this is the first description of IgA-MPT-64 and IgG Lionex antibodies in CSF-TBM and, although there is good specificity, adjustments are needed based on antigen composition to enhance sensitivity.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26134/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALisabela_sardella_etal_IOC_2010.pdfisabela_sardella_etal_IOC_2010.pdfapplication/pdf1162237https://www.arca.fiocruz.br/bitstream/icict/26134/2/isabela_sardella_etal_IOC_2010.pdf11c7761d4ce0750dbf5b1b69b063524bMD52TEXTisabela_sardella_etal_IOC_2010.pdf.txtisabela_sardella_etal_IOC_2010.pdf.txtExtracted texttext/plain37098https://www.arca.fiocruz.br/bitstream/icict/26134/3/isabela_sardella_etal_IOC_2010.pdf.txt94c820712faf4c64bce4dfadec280308MD53icict/261342018-08-15 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| dc.title.pt_BR.fl_str_mv |
Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients |
| title |
Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients |
| spellingShingle |
Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients Sardella, Isabela Gama Tuberculose Líquido Cefalorraquidiano Ensaio de Imunoadsorção Enzimática Mycobacterium tuberculosis Resposta imune tuberculosis cerebrospinal fluid ELISA meningoencephalitis tuberculosis Mycobacterium tuberculosis immune response |
| title_short |
Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients |
| title_full |
Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients |
| title_fullStr |
Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients |
| title_full_unstemmed |
Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients |
| title_sort |
Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients |
| author |
Sardella, Isabela Gama |
| author_facet |
Sardella, Isabela Gama Singh, Mahavir Kumpfer, Susanne Heringer, Rafael Ribeiro Saad, Maria Helena Féres Sohler, Marzia Puccioni |
| author_role |
author |
| author2 |
Singh, Mahavir Kumpfer, Susanne Heringer, Rafael Ribeiro Saad, Maria Helena Féres Sohler, Marzia Puccioni |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Sardella, Isabela Gama Singh, Mahavir Kumpfer, Susanne Heringer, Rafael Ribeiro Saad, Maria Helena Féres Sohler, Marzia Puccioni |
| dc.subject.other.pt_BR.fl_str_mv |
Tuberculose Líquido Cefalorraquidiano Ensaio de Imunoadsorção Enzimática Mycobacterium tuberculosis Resposta imune |
| topic |
Tuberculose Líquido Cefalorraquidiano Ensaio de Imunoadsorção Enzimática Mycobacterium tuberculosis Resposta imune tuberculosis cerebrospinal fluid ELISA meningoencephalitis tuberculosis Mycobacterium tuberculosis immune response |
| dc.subject.en.pt_BR.fl_str_mv |
tuberculosis cerebrospinal fluid ELISA meningoencephalitis tuberculosis Mycobacterium tuberculosis immune response |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. |
| publishDate |
2010 |
| dc.date.issued.fl_str_mv |
2010 |
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2018-04-26T14:53:33Z |
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2018-04-26T14:53:33Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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| dc.identifier.citation.fl_str_mv |
SARDELLA, Isabela Gama; et al. Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients. Mem Inst Oswaldo Cruz, v.105, n.5, p. 722-728, Aug. 2010. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/26134 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
SARDELLA, Isabela Gama; et al. Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients. Mem Inst Oswaldo Cruz, v.105, n.5, p. 722-728, Aug. 2010. 0074-0276 1678-8060 |
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https://www.arca.fiocruz.br/handle/icict/26134 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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