Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24709 |
Resumo: | Bahia State Agency for Research Funding (FAPESB), Brazil; Brazilian Council for Scientific and Technological Development (CNPq), Brazil; Turku University Hospital Research Foundation, Finland; Rauno and Anne Puolimatka Foundation, Finland; Sohlberg Foundation, Finland. |
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Andrade, Dafne CarvalhoBorges, Igor CarmoEkström, N.Jartti, T.Puhakka, T.Barral, Aldina Maria PradoKayhty, H.Ruuskanen, O.Carvalho, Cristiana Maria Costa Nascimento de2018-02-05T14:06:18Z2018-02-05T14:06:18Z2018ANDRADE, D. C. et al. Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings. European Journal of Clinical Microbiology and Infectious Diseases, out. 2017.0934-9723https://www.arca.fiocruz.br/handle/icict/2470910.1007/s10096-017-3103-8Bahia State Agency for Research Funding (FAPESB), Brazil; Brazilian Council for Scientific and Technological Development (CNPq), Brazil; Turku University Hospital Research Foundation, Finland; Rauno and Anne Puolimatka Foundation, Finland; Sohlberg Foundation, Finland.Federal University of Bahia. School of Medicine. Postgraduate Programme in Health Sciences. Salvador, BA, BrazilFederal University of Bahia. School of Medicine. Postgraduate Programme in Health Sciences. Salvador, BA, BrazilNational Institute for Health and Welfare. Helsinki, FinlandUniversity of Turku and Turku University Hospital. Department of Paediatrics. Turku, FinlandUniversity of Turku and Turku University Hospital. Department of Paediatrics. Turku, Finland / Satakunta Central Hospital. Department of Otorhinolaryngology. Pori, FinlandFederal University of Bahia. School of Medicine. Pathology Department and Postgraduate Programme in Health Sciences. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of Turku and Turku University Hospital. Department of Paediatrics. Turku, FinlandUniversity of Turku and Turku University Hospital. Department of Paediatrics. Turku, Finland / Satakunta Central Hospital. Department of Otorhinolaryngology. Pori, FinlandFederal University of Bahia. School of Medicine. Pathology Department and Postgraduate Programme in Health Sciences. Salvador, BA, BrazilThe measurement of antibody levels is a common test for the diagnosis of Streptococcus pneumoniae infection in research. However, the quality of antibody response, reflected by avidity, has not been adequately evaluated. We aimed to evaluate the role of avidity of IgG against eight pneumococcal proteins in etiologic diagnosis. Eight pneumococcal proteins (Ply, CbpA, PspA1 and 2, PcpA, PhtD, StkP-C, and PcsB-N) were used to develop a multiplex bead-based avidity immunoassay. The assay was tested for effects of the chaotropic agent, multiplexing, and repeatability. The developed assay was applied to paired samples from children with or without pneumococcal disease (n = 38 for each group), determined by either serology, polymerase chain reaction (PCR), or blood culture. We found a good correlation between singleplex and multiplex assays, with r ≥ 0.94.The assay was reproducible, with mean inter-assay variation ≤ 9% and intra-assay variation < 6%. Children with pneumococcal disease had lower median avidity indexes in the acute phase of disease for PspA1 and 2 (p = 0.042), PcpA (p = 0.002), PhtD (p = 0.014), and StkP-C (p < 0.001). When the use of IgG avidity as a diagnostic tool for pneumococcal infection was evaluated, the highest discriminative power was found for StkP-C, followed by PcpA (area under the curve [95% confidence interval, CI]: 0.868 [0.759-0.977] and 0.743 [0.607-879], respectively). The developed assay was robust and had no deleterious influence from multiplexing. Children with pneumococcal disease had lower median avidity against five pneumococcal proteins in the acute phase of disease compared to children without disease.engSpringer VerlagStreptococcus pneumoniaeInfecções pneumococicasProteínasCriançasStreptococcus pneumoniaeInfectionProteinChildrenDetermination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settingsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24709/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAndrade DC Determination of a vidity....pdfAndrade DC Determination of a vidity....pdfapplication/pdf616257https://www.arca.fiocruz.br/bitstream/icict/24709/2/Andrade%20DC%20Determination%20of%20a%20vidity....pdfe4039ee328b9171190615bc1efe09fa7MD52TEXTAndrade DC Determination of a vidity....pdf.txtAndrade DC Determination of a vidity....pdf.txtExtracted texttext/plain54175https://www.arca.fiocruz.br/bitstream/icict/24709/3/Andrade%20DC%20Determination%20of%20a%20vidity....pdf.txt9afc900850902e66f92dc3d1d2adc009MD53icict/247092023-03-15 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| dc.title.pt_BR.fl_str_mv |
Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings |
| title |
Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings |
| spellingShingle |
Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings Andrade, Dafne Carvalho Streptococcus pneumoniae Infecções pneumococicas Proteínas Crianças Streptococcus pneumoniae Infection Protein Children |
| title_short |
Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings |
| title_full |
Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings |
| title_fullStr |
Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings |
| title_full_unstemmed |
Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings |
| title_sort |
Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings |
| author |
Andrade, Dafne Carvalho |
| author_facet |
Andrade, Dafne Carvalho Borges, Igor Carmo Ekström, N. Jartti, T. Puhakka, T. Barral, Aldina Maria Prado Kayhty, H. Ruuskanen, O. Carvalho, Cristiana Maria Costa Nascimento de |
| author_role |
author |
| author2 |
Borges, Igor Carmo Ekström, N. Jartti, T. Puhakka, T. Barral, Aldina Maria Prado Kayhty, H. Ruuskanen, O. Carvalho, Cristiana Maria Costa Nascimento de |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Andrade, Dafne Carvalho Borges, Igor Carmo Ekström, N. Jartti, T. Puhakka, T. Barral, Aldina Maria Prado Kayhty, H. Ruuskanen, O. Carvalho, Cristiana Maria Costa Nascimento de |
| dc.subject.other.pt_BR.fl_str_mv |
Streptococcus pneumoniae Infecções pneumococicas Proteínas Crianças |
| topic |
Streptococcus pneumoniae Infecções pneumococicas Proteínas Crianças Streptococcus pneumoniae Infection Protein Children |
| dc.subject.en.pt_BR.fl_str_mv |
Streptococcus pneumoniae Infection Protein Children |
| description |
Bahia State Agency for Research Funding (FAPESB), Brazil; Brazilian Council for Scientific and Technological Development (CNPq), Brazil; Turku University Hospital Research Foundation, Finland; Rauno and Anne Puolimatka Foundation, Finland; Sohlberg Foundation, Finland. |
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2018 |
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2018-02-05T14:06:18Z |
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2018-02-05T14:06:18Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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ANDRADE, D. C. et al. Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings. European Journal of Clinical Microbiology and Infectious Diseases, out. 2017. |
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https://www.arca.fiocruz.br/handle/icict/24709 |
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0934-9723 |
| dc.identifier.doi.none.fl_str_mv |
10.1007/s10096-017-3103-8 |
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ANDRADE, D. C. et al. Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings. European Journal of Clinical Microbiology and Infectious Diseases, out. 2017. 0934-9723 10.1007/s10096-017-3103-8 |
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