Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018

Detalhes bibliográficos
Autor(a) principal: Manta, Fernanda S. N.
Data de Publicação: 2019
Outros Autores: Barbieri, Raquel R., Moreira, Suelen J. M., Santos, Paulo T. S., Nery, José A. C., Duppre, Nádia C., Sales, Anna M., Pacheco, Antonio G., Hacker, Mariana A., Machado, Alice M., Sarno, Euzenir N., Moraes, Milton O
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45079
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
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spelling Manta, Fernanda S. N.Barbieri, Raquel R.Moreira, Suelen J. M.Santos, Paulo T. S.Nery, José A. C.Duppre, Nádia C.Sales, Anna M.Pacheco, Antonio G.Hacker, Mariana A.Machado, Alice M.Sarno, Euzenir N.Moraes, Milton O,2020-12-22T18:26:15Z2020-12-22T18:26:15Z2019MANTA, Fernanda S. N. et al. Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study. 2011-2018. Scientific Reports, v. 9, p. 1-8, 2019.2045-2322https://www.arca.fiocruz.br/handle/icict/4507910.1038/s41598-019-52640-5engNature ResearchPCR QuantitativoDiagnósticoMonitoramentoHanseníaseContatos familiaresEstudos de acompanhamentoQuantitative PCRLeprosyDiagnosisMonitoringHousehold contactsFollow-up studiesQuantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Presidência. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Household contacts (HHC) of leprosy patients exhibit high-risk of developing leprosy and contact tracing is helpful for early diagnosis. From 2011 to 2018,2,437 HHC were examined in a clinic in Rio de Janeiro, Brazil and 16S qPCR was used for diagnosis and monitoring of contacts. Fifty-four HHCs were clinically diagnosed with leprosy at intake. Another 25 exhibited leprosy-like skin lesions at intake, 8 of which were confirmed as having leprosy (50% of which were qPCR positive) and 17 of which were diagnosed with other skin diseases (6% qPCR positive). In skin biopsies, qPCR presented a sensitivity of 0.50 and specificity of 0.94. Furthermore, 955 healthy HHCs were followed-up for at least 3 years and skin scrapings were collected from earlobes for qPCR detection. Positive qPCR indicated a non-significant relative risk of 2.52 of developing the disease. During follow-up, those who progressed towards leprosy exhibited 20% qPCR positivity, compared to 9% of those who remained healthy. Disease-free survival rates indicated that age had a significant impact on disease progression, where patients over 60 had a greater chance of developing leprosy [HR = 32.4 (3.6–290.3)]. Contact tracing combined with qPCR may assist in early diagnosis and age is a risk factor for leprosy progression.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45079/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALEuzenirSarno_AnnaSalles_etal_IOC_2020.pdfEuzenirSarno_AnnaSalles_etal_IOC_2020.pdfapplication/pdf1240132https://www.arca.fiocruz.br/bitstream/icict/45079/2/EuzenirSarno_AnnaSalles_etal_IOC_2020.pdf83cc06c7eb65522c24e124520d2fd275MD52TEXTEuzenirSarno_AnnaSalles_etal_IOC_2020.pdf.txtEuzenirSarno_AnnaSalles_etal_IOC_2020.pdf.txtExtracted texttext/plain33310https://www.arca.fiocruz.br/bitstream/icict/45079/3/EuzenirSarno_AnnaSalles_etal_IOC_2020.pdf.txtc046257bfe3b4b44978a2c77f63cc439MD53icict/450792022-06-24 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dc.title.pt_BR.fl_str_mv Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018
title Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018
spellingShingle Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018
Manta, Fernanda S. N.
PCR Quantitativo
Diagnóstico
Monitoramento
Hanseníase
Contatos familiares
Estudos de acompanhamento
Quantitative PCR
Leprosy
Diagnosis
Monitoring
Household contacts
Follow-up studies
title_short Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018
title_full Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018
title_fullStr Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018
title_full_unstemmed Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018
title_sort Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study, 2011–2018
author Manta, Fernanda S. N.
author_facet Manta, Fernanda S. N.
Barbieri, Raquel R.
Moreira, Suelen J. M.
Santos, Paulo T. S.
Nery, José A. C.
Duppre, Nádia C.
Sales, Anna M.
Pacheco, Antonio G.
Hacker, Mariana A.
Machado, Alice M.
Sarno, Euzenir N.
Moraes, Milton O,
author_role author
author2 Barbieri, Raquel R.
Moreira, Suelen J. M.
Santos, Paulo T. S.
Nery, José A. C.
Duppre, Nádia C.
Sales, Anna M.
Pacheco, Antonio G.
Hacker, Mariana A.
Machado, Alice M.
Sarno, Euzenir N.
Moraes, Milton O,
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Manta, Fernanda S. N.
Barbieri, Raquel R.
Moreira, Suelen J. M.
Santos, Paulo T. S.
Nery, José A. C.
Duppre, Nádia C.
Sales, Anna M.
Pacheco, Antonio G.
Hacker, Mariana A.
Machado, Alice M.
Sarno, Euzenir N.
Moraes, Milton O,
dc.subject.other.pt_BR.fl_str_mv PCR Quantitativo
Diagnóstico
Monitoramento
Hanseníase
Contatos familiares
Estudos de acompanhamento
topic PCR Quantitativo
Diagnóstico
Monitoramento
Hanseníase
Contatos familiares
Estudos de acompanhamento
Quantitative PCR
Leprosy
Diagnosis
Monitoring
Household contacts
Follow-up studies
dc.subject.en.pt_BR.fl_str_mv Quantitative PCR
Leprosy
Diagnosis
Monitoring
Household contacts
Follow-up studies
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-12-22T18:26:15Z
dc.date.available.fl_str_mv 2020-12-22T18:26:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MANTA, Fernanda S. N. et al. Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study. 2011-2018. Scientific Reports, v. 9, p. 1-8, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45079
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-019-52640-5
identifier_str_mv MANTA, Fernanda S. N. et al. Quantitative PCR for leprosy diagnosis and monitoring in household contacts: A follow-up study. 2011-2018. Scientific Reports, v. 9, p. 1-8, 2019.
2045-2322
10.1038/s41598-019-52640-5
url https://www.arca.fiocruz.br/handle/icict/45079
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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