Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Detalhes bibliográficos
Autor(a) principal: Chagas, Thiago Pavoni Gomes
Data de Publicação: 2011
Outros Autores: Alves, Ronaldo Mendes, Vallim, Deyse Christina, Seki, Liliane Miyuki, Campos, Leila Carvalho, Asensi, Marise Dutra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19078
Resumo: FAPERJ e CNPq.
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spelling Chagas, Thiago Pavoni GomesAlves, Ronaldo MendesVallim, Deyse ChristinaSeki, Liliane MiyukiCampos, Leila CarvalhoAsensi, Marise Dutra2017-05-31T18:49:22Z2017-05-31T18:49:22Z2011CHAGAS, T. P. G. et al. Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil. Brazilian Journal of Infectious Diseases, v. 15, n. 5, p. 420-425, 2011.1413-8670https://www.arca.fiocruz.br/handle/icict/19078FAPERJ e CNPq.Universidade do Estado do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilUniversidade Rural do Rio de Janeiro. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilHospital Infection Research Laboratory. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilObjective: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil. Material and Methods: Eighty-five K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test and the Etest®. The MIC50 and MIC90 for ESBL-producing isolates were determined by the Etest® method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion method according to the CLSI. Screening for blaTEM, blaSHV, blaCTX-M genes and class 1 integron was performed by PCR amplification. To determine the genomic diversity of CTX-M-producers, isolates were analyzed by macrorestriction profile analysis following PFGE. Results and Discussion: Seventy-one K. pneumoniae isolates were ESBL-producing. PCR and sequencing experiments detected 38 CTX-M-producing K. pneumoniae belonged to groups CTX-M 1, CTX-M 2, CTX-M 8 and CTX-M 9. The association of different types ESBL (CTX-M, SHV and TEM) was frequent. All K. pneumoniae isolates carried class 1 integron. PFGE analysis revealed thirty-one clonal types among CTX-M-producing isolates. The data presented herein illustrate the diversity of genotypes of CTX-M producing K. pneumoniae among Brazilians hospitals.engElsevierKlebsiella pneumoniaeΒ-lactamasesGenótipoKlebsiella pneumoniaeβ-lactamasesGenotypeKlebsiella pneumoniaeBeta lactamasesGenótipoDiversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19078/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALChagas TPG Diversity of genotypes....pdfChagas TPG Diversity of genotypes....pdfapplication/pdf375018https://www.arca.fiocruz.br/bitstream/icict/19078/2/Chagas%20TPG%20Diversity%20of%20genotypes....pdfb38408ee64835a41251fd3b32e96340bMD52TEXTChagas TPG 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dc.title.pt_BR.fl_str_mv Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil
title Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil
spellingShingle Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil
Chagas, Thiago Pavoni Gomes
Klebsiella pneumoniae
Β-lactamases
Genótipo
Klebsiella pneumoniae
β-lactamases
Genotype
Klebsiella pneumoniae
Beta lactamases
Genótipo
title_short Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil
title_full Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil
title_fullStr Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil
title_full_unstemmed Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil
title_sort Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil
author Chagas, Thiago Pavoni Gomes
author_facet Chagas, Thiago Pavoni Gomes
Alves, Ronaldo Mendes
Vallim, Deyse Christina
Seki, Liliane Miyuki
Campos, Leila Carvalho
Asensi, Marise Dutra
author_role author
author2 Alves, Ronaldo Mendes
Vallim, Deyse Christina
Seki, Liliane Miyuki
Campos, Leila Carvalho
Asensi, Marise Dutra
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Chagas, Thiago Pavoni Gomes
Alves, Ronaldo Mendes
Vallim, Deyse Christina
Seki, Liliane Miyuki
Campos, Leila Carvalho
Asensi, Marise Dutra
dc.subject.other.pt_BR.fl_str_mv Klebsiella pneumoniae
Β-lactamases
Genótipo
topic Klebsiella pneumoniae
Β-lactamases
Genótipo
Klebsiella pneumoniae
β-lactamases
Genotype
Klebsiella pneumoniae
Beta lactamases
Genótipo
dc.subject.en.pt_BR.fl_str_mv Klebsiella pneumoniae
β-lactamases
Genotype
dc.subject.decs.pt_BR.fl_str_mv Klebsiella pneumoniae
Beta lactamases
Genótipo
description FAPERJ e CNPq.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2017-05-31T18:49:22Z
dc.date.available.fl_str_mv 2017-05-31T18:49:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CHAGAS, T. P. G. et al. Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil. Brazilian Journal of Infectious Diseases, v. 15, n. 5, p. 420-425, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19078
dc.identifier.issn.pt_BR.fl_str_mv 1413-8670
identifier_str_mv CHAGAS, T. P. G. et al. Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil. Brazilian Journal of Infectious Diseases, v. 15, n. 5, p. 420-425, 2011.
1413-8670
url https://www.arca.fiocruz.br/handle/icict/19078
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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