Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1

Detalhes bibliográficos
Autor(a) principal: Silva, Marcio Roberto
Data de Publicação: 2011
Outros Autores: Guimarães, Mark Drew Crosland, Oliveira, Vania Maria de, Moreira, Aline dos Santos, Costa, Ronaldo Rodrigues da, Abi-Zaid, Kelly Cristina Ferreira, Rocha, Adalgiza da Silva, Suffys, Philip Noel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16188
Resumo: Ministério da Agricultura, Pecuária e Abastecimento. EMBRAPA Gado de Leite. Juiz de Fora, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Medicina Preventiva e Social. Programa de Pós-Graduação em Saúde Pública. Belo Horizonte, MG, Brasil.
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spelling Silva, Marcio RobertoGuimarães, Mark Drew CroslandOliveira, Vania Maria deMoreira, Aline dos SantosCosta, Ronaldo Rodrigues daAbi-Zaid, Kelly Cristina FerreiraRocha, Adalgiza da SilvaSuffys, Philip Noel2016-10-11T15:09:13Z2016-10-11T15:09:13Z2011SILVA, Marcio Roberto; et al. Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1. Mem Inst Oswaldo Cruz, v.106, n.1, p.9-15, Feb. 2011.0074-0276https://www.arca.fiocruz.br/handle/icict/161881678-8060engFundação Oswaldo CruzMycobacterium tuberculosisMycobacterium bovisReação em Cadeia da PolimerasebaciloscopiaDados de seqüência molecularoxyR pseudogenepolymerase chain reactionsputum smearMycobacterium tuberculosisMycobacterium bovismolecular sequence dataIdentification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMinistério da Agricultura, Pecuária e Abastecimento. EMBRAPA Gado de Leite. Juiz de Fora, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Medicina Preventiva e Social. Programa de Pós-Graduação em Saúde Pública. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Medicina Preventiva e Social. Programa de Pós-Graduação em Saúde Pública. Belo Horizonte, MG, Brasil.Ministério da Agricultura, Pecuária e Abastecimento. EMBRAPA Gado de Leite. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Universidade Federal de Juiz de Fora. Hospital Universitário. Juiz de Fora, MG, Brasil / Fundação Hospitalar do Estado de Minas. Hospital João Penido. Juiz de Fora, MG, Brasil.Fundação Hospitalar do Estado de Minas. Hospital João Penido. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.A cross-sectional analysis of stored Ziehl-Neelsen (ZN)-stained sputum smear slides (SSS) obtained from two public tuberculosis referral laboratories located in Juiz de Fora, Minas Gerais, was carried out to distinguish Mycobacterium bovis from other members of the Mycobacterium tuberculosis complex (MTC). A two-step approach was used to distinguish M. bovis from other members of MTC: (i) oxyR pseudogene amplification to detect MTC and, subsequently, (ii) allele-specific sequencing based on the polymorphism at position 285 of this gene. The oxyR pseudogene was successfully amplified in 100 of 177 (56.5%) SSS available from 99 individuals. No molecular profile of M. bovis was found. Multivariate analysis indicated that acid-fast bacilli (AFB) results and the source laboratory were associated (p < 0.05) with oxyR pseudogene amplification. SSS that were AFB++ SSS showed more oxyR pseudogene amplification than those with AFB0, possibly due to the amount of DNA. One of the two source laboratories presented a greater chance of oxyR pseudogene amplification, suggesting that differences in sputum conservation between laboratories could have influenced the preservation of DNA. This study provides evidence that stored ZN-SSS can be used for the molecular detection of MTC.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16188/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALphylip2_suffys_etal_IOC_2011.pdfphylip2_suffys_etal_IOC_2011.pdfapplication/pdf646758https://www.arca.fiocruz.br/bitstream/icict/16188/2/phylip2_suffys_etal_IOC_2011.pdf0a786abac8106330f5a9809fc4c6b69aMD52TEXTphylip2_suffys_etal_IOC_2011.pdf.txtphylip2_suffys_etal_IOC_2011.pdf.txtExtracted texttext/plain35825https://www.arca.fiocruz.br/bitstream/icict/16188/3/phylip2_suffys_etal_IOC_2011.pdf.txt6086a7ae550603048f4070fe022c1d09MD53icict/161882018-04-02 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dc.title.pt_BR.fl_str_mv Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1
title Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1
spellingShingle Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1
Silva, Marcio Roberto
Mycobacterium tuberculosis
Mycobacterium bovis
Reação em Cadeia da Polimerase
baciloscopia
Dados de seqüência molecular
oxyR pseudogene
polymerase chain reaction
sputum smear
Mycobacterium tuberculosis
Mycobacterium bovis
molecular sequence data
title_short Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1
title_full Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1
title_fullStr Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1
title_full_unstemmed Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1
title_sort Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1
author Silva, Marcio Roberto
author_facet Silva, Marcio Roberto
Guimarães, Mark Drew Crosland
Oliveira, Vania Maria de
Moreira, Aline dos Santos
Costa, Ronaldo Rodrigues da
Abi-Zaid, Kelly Cristina Ferreira
Rocha, Adalgiza da Silva
Suffys, Philip Noel
author_role author
author2 Guimarães, Mark Drew Crosland
Oliveira, Vania Maria de
Moreira, Aline dos Santos
Costa, Ronaldo Rodrigues da
Abi-Zaid, Kelly Cristina Ferreira
Rocha, Adalgiza da Silva
Suffys, Philip Noel
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Marcio Roberto
Guimarães, Mark Drew Crosland
Oliveira, Vania Maria de
Moreira, Aline dos Santos
Costa, Ronaldo Rodrigues da
Abi-Zaid, Kelly Cristina Ferreira
Rocha, Adalgiza da Silva
Suffys, Philip Noel
dc.subject.other.pt_BR.fl_str_mv Mycobacterium tuberculosis
Mycobacterium bovis
Reação em Cadeia da Polimerase
baciloscopia
Dados de seqüência molecular
topic Mycobacterium tuberculosis
Mycobacterium bovis
Reação em Cadeia da Polimerase
baciloscopia
Dados de seqüência molecular
oxyR pseudogene
polymerase chain reaction
sputum smear
Mycobacterium tuberculosis
Mycobacterium bovis
molecular sequence data
dc.subject.en.pt_BR.fl_str_mv oxyR pseudogene
polymerase chain reaction
sputum smear
Mycobacterium tuberculosis
Mycobacterium bovis
molecular sequence data
description Ministério da Agricultura, Pecuária e Abastecimento. EMBRAPA Gado de Leite. Juiz de Fora, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Medicina Preventiva e Social. Programa de Pós-Graduação em Saúde Pública. Belo Horizonte, MG, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2016-10-11T15:09:13Z
dc.date.available.fl_str_mv 2016-10-11T15:09:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Marcio Roberto; et al. Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1. Mem Inst Oswaldo Cruz, v.106, n.1, p.9-15, Feb. 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16188
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv SILVA, Marcio Roberto; et al. Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1. Mem Inst Oswaldo Cruz, v.106, n.1, p.9-15, Feb. 2011.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/16188
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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