Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis

Detalhes bibliográficos
Autor(a) principal: Ferreira, Gabriella Freitas
Data de Publicação: 2012
Outros Autores: Moraes, Cícero, Silveira, Alda Maria Soares da, Oliveira, Rodrigo Corrêa de, Carvalho, Andréa Teixeira de, Martins Filho, Olindo Assis, Moreno, Elizabeth Castro, Carmo, Luiz Simeão do, Fraga, Lucia Alves de Oliveira, Malaquias, Luiz Cosme Cotta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7432
Resumo: Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil/Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Programa de Pós-Graduação em Ciências Biológicas. Governador Valadares, MG, Brasil
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spelling Ferreira, Gabriella FreitasMoraes, CíceroSilveira, Alda Maria Soares daOliveira, Rodrigo Corrêa deCarvalho, Andréa Teixeira deMartins Filho, Olindo AssisMoreno, Elizabeth CastroCarmo, Luiz Simeão doFraga, Lucia Alves de OliveiraMalaquias, Luiz Cosme Cotta2014-03-21T12:42:24Z2014-03-21T12:42:24Z2012FERREIRA, Freitas Ferreira et al. Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, n.3, pp. 348-355.0074-0276https://www.arca.fiocruz.br/handle/icict/743210.1590/S0074-02762012000300009engFundação Oswaldo CruzDistinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil/Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Programa de Pós-Graduação em Ciências Biológicas. Governador Valadares, MG, BrasilUniversidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil/Hospital Municipal. Governador Valadares, MG, BrasilUniversidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil/Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Programa de Pós-Graduação em Ciências Biológicas. Governador Valadares, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Hemominas. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia e Microbiologia. Belo Horizonte, MG, BrasilUniversidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil/Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Programa de Pós-Graduação em Ciências Biológicas. Governador Valadares, MG, BrasilUniversidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil/ Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Programa de Pós-Graduação em Ciências Biológicas. Governador Valadares, MG, Brasil/Universidade Federal de Alfenas. Departamento de Ciências Biomédicas. Laboratório de Microbiologia e Imunologia Básica. Alfenas, MG, BrasilWe investigated the cytokine profile of peripheral mononuclear cells from chronic osteomyelitis (OST) patients following in vitro stimulation with staphylococcal enterotoxin A (SEA). We demonstrate that stimulation with SEA induced prominent lymphocyte proliferation and high levels of tumour necrosis factor (TNF)-α, interleukin (IL)-4 and IL-10 secretion in both OST and non-infected individuals (NI). Even though stimulation with SEA had no impact on IL-6 production in either patient group, the baseline level of IL-6 production by cells from OST patients was always significantly less than that produced by cells from NI. After classifying the osteomyelitic episodes based on the time after the last reactivation event as "early" (1-4 months) or "late" osteomyelitis (5-12 months), we found that increased levels of TNF-α and IL-4 in combination with decreased levels of IL-6 were observed in the early episodes. By contrast, increased levels of IL-10, IL-2 and IL-6 were hallmarks of late episodes. Our data demonstrate that early osteomyelitic episodes are accompanied by an increased frequency of "high producers" of TNF-α and IL-4, whereas late events are characterised by increased frequencies of "high producers" of IL-10, IL-6 and IL-2. These findings demonstrate the distinct cytokine profiles in chronic osteomyelitis, with a distinct regulation of IL-6 production during early and late episodes.Staphylococcus aureusenterotoxin Achronic osteomyelitiscytokinesIL-6info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALDistinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis.pdfDistinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis.pdfapplication/pdf1677552https://www.arca.fiocruz.br/bitstream/icict/7432/1/Distinct%20cytokine%20profiles%20of%20circulating%20mononuclear%20cells%20stimulated%20with%20Staphylococcus%20aureus%20enterotoxin%20A%20in%20vitro%20during%20early%20and%20late%20episodes%20of%20chronic%20osteomyelitis.pdfd678398526db067c26b60e1f9d2cd29dMD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis
title Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis
spellingShingle Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis
Ferreira, Gabriella Freitas
Staphylococcus aureus
enterotoxin A
chronic osteomyelitis
cytokines
IL-6
title_short Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis
title_full Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis
title_fullStr Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis
title_full_unstemmed Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis
title_sort Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis
author Ferreira, Gabriella Freitas
author_facet Ferreira, Gabriella Freitas
Moraes, Cícero
Silveira, Alda Maria Soares da
Oliveira, Rodrigo Corrêa de
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
Moreno, Elizabeth Castro
Carmo, Luiz Simeão do
Fraga, Lucia Alves de Oliveira
Malaquias, Luiz Cosme Cotta
author_role author
author2 Moraes, Cícero
Silveira, Alda Maria Soares da
Oliveira, Rodrigo Corrêa de
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
Moreno, Elizabeth Castro
Carmo, Luiz Simeão do
Fraga, Lucia Alves de Oliveira
Malaquias, Luiz Cosme Cotta
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Gabriella Freitas
Moraes, Cícero
Silveira, Alda Maria Soares da
Oliveira, Rodrigo Corrêa de
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
Moreno, Elizabeth Castro
Carmo, Luiz Simeão do
Fraga, Lucia Alves de Oliveira
Malaquias, Luiz Cosme Cotta
dc.subject.en.pt_BR.fl_str_mv Staphylococcus aureus
enterotoxin A
chronic osteomyelitis
cytokines
IL-6
topic Staphylococcus aureus
enterotoxin A
chronic osteomyelitis
cytokines
IL-6
description Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Governador Valadares, MG, Brasil/Universidade Vale do Rio Doce. Núcleo de Pesquisa em Imunologia. Programa de Pós-Graduação em Ciências Biológicas. Governador Valadares, MG, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-03-21T12:42:24Z
dc.date.available.fl_str_mv 2014-03-21T12:42:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERREIRA, Freitas Ferreira et al. Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, n.3, pp. 348-355.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7432
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762012000300009
identifier_str_mv FERREIRA, Freitas Ferreira et al. Distinct cytokine profiles of circulating mononuclear cells stimulated with Staphylococcus aureus enterotoxin A in vitro during early and late episodes of chronic osteomyelitis. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, n.3, pp. 348-355.
0074-0276
10.1590/S0074-02762012000300009
url https://www.arca.fiocruz.br/handle/icict/7432
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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