Leishmania amazonensis: characterization of an ecto-3´-nucleotidase activity and its possible role in virulence
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/9867 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil |
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Silva, Rafael PalettaVieira, Danielle PBerbardo, Rodrigo VieiraMajerowicz, DavidGondim, Katia CalpSantos, Marcos André Vannier dosLopes, Angela Hampshire de Carvalho SantosFernandes, José Roberto Meyer2015-03-31T16:27:40Z2015-03-31T16:27:40Z2011PALETTA-SILVA, R. et al. Leishmania amazonensis: characterization of an ecto-3'-nucleotidase activity and its possible role in virulence. Experimental Parasitology, v. 129, n. 3, p.277-283,, 2011.0014-4894https://www.arca.fiocruz.br/handle/icict/986710.1016/j.exppara.2011.07.014engElsevierLeishmania amazonensis: characterization of an ecto-3´-nucleotidase activity and its possible role in virulenceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, BrasilUniversidade Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, BrasilEcto-30-nucleotidase/nuclease (30NT/NU) is a membrane-bound enzyme that plays a key role in the nutrition of Leishmania sp. protozoan parasites. This enzyme generates nucleosides via hydrolyzes of 30mononucleotides and nucleic acids, which enter the cell by specific transporters. In this work, we identify and characterize Leishmania amazonensis ecto-30-nucleotidase activity (La30-nucleotidase), report ammonium tetrathiomolybdate (TTM) as a novel La30-nucleotidase inhibitor and approach the possible involvement of ecto-30-nucleotidase in cellular adhesion. La30-nucleotidase presented characteristics similar to those reported for the class I single-strand nuclease family; a molecular weight of approximately 40 kDa and optimum activity in an alkaline pH range were observed. Although it is conserved among the genus, La30-nucleotidase displays different kinetic properties; it can be inhibited by vanadate, molybdate and Cu2+ ions. Interestingly, ecto-30-nucleotidase activity is 60-fold higher than that of ecto-50-nucleotidase in L. amazonensis. Additionally, ecto-30-nucleotidase activity is two-fold higher in virulent L. amazonensis cells than in avirulent ones. Notably, macrophage–parasite attachment/invasion was increased by 400% in the presence of adenosine 30-monophosphate (30AMP); however, this effect was reverted by TTM treatment. We believe that La30-nucleotidase may play a significant role in the generation of adenosine, which may contribute to mammalian host immune response impairment and establishment of infectionEcto-30-nucleotidaseEcto-50-nucleotidaseLeishmania amazonensisCellular adhesionAdenosineAmmonium tetrathiomolybdateLeishmania mexicana/enzimologiaLeishmania mexicana/patogenicidadeMacrófagos Peritoneais/parasitologiaNucleotidases/metabolismoMonofosfato de AdenosinaVirulenciainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/9867/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALPalleta-Silva R Leishmania amazonienses.....pdfPalleta-Silva R Leishmania amazonienses.....pdfapplication/pdf603227https://www.arca.fiocruz.br/bitstream/icict/9867/2/Palleta-Silva%20R%20Leishmania%20amazonienses.....pdf83ce2fdf0647e6b62e497920ee4e59ccMD52TEXTPalleta-Silva R Leishmania amazonienses.....pdf.txtPalleta-Silva R Leishmania amazonienses.....pdf.txtExtracted texttext/plain44814https://www.arca.fiocruz.br/bitstream/icict/9867/3/Palleta-Silva%20R%20Leishmania%20amazonienses.....pdf.txtfd1c2319a71f718ef06d10259b839cbaMD53icict/98672023-03-15 14:33:23.269oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Leishmania amazonensis: characterization of an ecto-3´-nucleotidase activity and its possible role in virulence |
title |
Leishmania amazonensis: characterization of an ecto-3´-nucleotidase activity and its possible role in virulence |
spellingShingle |
Leishmania amazonensis: characterization of an ecto-3´-nucleotidase activity and its possible role in virulence Silva, Rafael Paletta Ecto-30-nucleotidase Ecto-50-nucleotidase Leishmania amazonensis Cellular adhesion Adenosine Ammonium tetrathiomolybdate Leishmania mexicana/enzimologia Leishmania mexicana/patogenicidade Macrófagos Peritoneais/parasitologia Nucleotidases/metabolismo Monofosfato de Adenosina Virulencia |
title_short |
Leishmania amazonensis: characterization of an ecto-3´-nucleotidase activity and its possible role in virulence |
title_full |
Leishmania amazonensis: characterization of an ecto-3´-nucleotidase activity and its possible role in virulence |
title_fullStr |
Leishmania amazonensis: characterization of an ecto-3´-nucleotidase activity and its possible role in virulence |
title_full_unstemmed |
Leishmania amazonensis: characterization of an ecto-3´-nucleotidase activity and its possible role in virulence |
title_sort |
Leishmania amazonensis: characterization of an ecto-3´-nucleotidase activity and its possible role in virulence |
author |
Silva, Rafael Paletta |
author_facet |
Silva, Rafael Paletta Vieira, Danielle P Berbardo, Rodrigo Vieira Majerowicz, David Gondim, Katia Calp Santos, Marcos André Vannier dos Lopes, Angela Hampshire de Carvalho Santos Fernandes, José Roberto Meyer |
author_role |
author |
author2 |
Vieira, Danielle P Berbardo, Rodrigo Vieira Majerowicz, David Gondim, Katia Calp Santos, Marcos André Vannier dos Lopes, Angela Hampshire de Carvalho Santos Fernandes, José Roberto Meyer |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Rafael Paletta Vieira, Danielle P Berbardo, Rodrigo Vieira Majerowicz, David Gondim, Katia Calp Santos, Marcos André Vannier dos Lopes, Angela Hampshire de Carvalho Santos Fernandes, José Roberto Meyer |
dc.subject.en.pt_BR.fl_str_mv |
Ecto-30-nucleotidase Ecto-50-nucleotidase Leishmania amazonensis Cellular adhesion Adenosine Ammonium tetrathiomolybdate |
topic |
Ecto-30-nucleotidase Ecto-50-nucleotidase Leishmania amazonensis Cellular adhesion Adenosine Ammonium tetrathiomolybdate Leishmania mexicana/enzimologia Leishmania mexicana/patogenicidade Macrófagos Peritoneais/parasitologia Nucleotidases/metabolismo Monofosfato de Adenosina Virulencia |
dc.subject.decs.pt_BR.fl_str_mv |
Leishmania mexicana/enzimologia Leishmania mexicana/patogenicidade Macrófagos Peritoneais/parasitologia Nucleotidases/metabolismo Monofosfato de Adenosina Virulencia |
description |
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2015-03-31T16:27:40Z |
dc.date.available.fl_str_mv |
2015-03-31T16:27:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PALETTA-SILVA, R. et al. Leishmania amazonensis: characterization of an ecto-3'-nucleotidase activity and its possible role in virulence. Experimental Parasitology, v. 129, n. 3, p.277-283,, 2011. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/9867 |
dc.identifier.issn.none.fl_str_mv |
0014-4894 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.exppara.2011.07.014 |
identifier_str_mv |
PALETTA-SILVA, R. et al. Leishmania amazonensis: characterization of an ecto-3'-nucleotidase activity and its possible role in virulence. Experimental Parasitology, v. 129, n. 3, p.277-283,, 2011. 0014-4894 10.1016/j.exppara.2011.07.014 |
url |
https://www.arca.fiocruz.br/handle/icict/9867 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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