Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23203 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases. Rio de Janeiro, RJ, Brasil. |
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Oliveira, Simone Santiago Carvalho deGonçalves, Diego de SouzaGomes, Aline dos Santos GarciaGonçalves, Inês CorreaSeabra, Sergio HenriqueMenna-Barreto, Rubem FigueiredoLopes, Angela Hampshire de Carvalho SantosLevy, Claudia Masini d'AvilaSantos, André Luis Souza dosBranquinha, Marta Helena2017-11-16T12:33:03Z2017-11-16T12:33:03Z2017OLIVEIRA, Simone Santiago Carvalho de; et al. Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host. Mem Inst Oswaldo Cruz, v. 112, n.1, p. 31-43, Jan. 2017.0074-0276https://www.arca.fiocruz.br/handle/icict/2320310.1590/0074-027601602701678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzPhytomonas serpensParasitosPhytomonascalpain-like proteinscysteine peptidasecruzipainGp63Oncopeltus fasciatusSusceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate hostinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química, Programa de Pós-Graduação em Bioquímica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários. Rio de Janeiro, RJ, Brasil / Instituto Federal de Educação, Ciência e Tecnologia. Laboratório de Microbiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Bioquímica de Microrganismos. Rio de Janeiro, RJ, Brasil.Centro Universitário Estadual da Zona Oeste. Laboratório de Tecnologia em Cultura de Células. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Bioquímica de Microrganismos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Coleção de Protozoários. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Química, Programa de Pós-Graduação em Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases. Rio de Janeiro, RJ, Brasil.A pleiotropic response to the calpain inhibitor MDL28170 was detected in the tomato parasite Phytomonas serpens. Ultrastructural studies revealed that MDL28170 caused mitochondrial swelling, shortening of flagellum and disruption of trans Golgi network. This effect was correlated to the inhibition in processing of cruzipain-like molecules, which presented an increase in expression paralleled by decreased proteolytic activity. Concomitantly, a calcium-dependent cysteine peptidase was detected in the parasite extract, the activity of which was repressed by pre-incubation of parasites with MDL28170. Flow cytometry and Western blotting analyses revealed the differential expression of calpain-like proteins (CALPs) in response to the pre-incubation of parasites with the MDL28170, and confocal fluorescence microscopy confirmed their surface location. The interaction of promastigotes with explanted salivary glands of the insect Oncopeltus fasciatus was reduced when parasites were pre-treated with MDL28170, which was correlated to reduced levels of surface cruzipain-like and gp63-like molecules. Treatment of parasites with anti-Drosophila melanogaster (Dm) calpain antibody also decreased the adhesion process. Additionally, parasites recovered from the interaction process presented higher levels of surface cruzipain-like and gp63-like molecules, with similar levels of CALPs cross-reactive to anti-Dm-calpain antibody. The results confirm the importance of exploring the use of calpain inhibitors in studying parasites' physiology.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23203/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrubemf_mennabarreto_etal_IOC_2017.pdfrubemf_mennabarreto_etal_IOC_2017.pdfapplication/pdf1321524https://www.arca.fiocruz.br/bitstream/icict/23203/2/rubemf_mennabarreto_etal_IOC_2017.pdff7d52d5ca2f7682d5b72e0c39967d61aMD52TEXTrubemf_mennabarreto_etal_IOC_2017.pdf.txtrubemf_mennabarreto_etal_IOC_2017.pdf.txtExtracted texttext/plain76160https://www.arca.fiocruz.br/bitstream/icict/23203/3/rubemf_mennabarreto_etal_IOC_2017.pdf.txtd32f06a215d6e158eb5e4876e0152806MD53icict/232032022-06-24 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| dc.title.pt_BR.fl_str_mv |
Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host |
| title |
Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host |
| spellingShingle |
Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host Oliveira, Simone Santiago Carvalho de Phytomonas serpens Parasitos Phytomonas calpain-like proteins cysteine peptidase cruzipain Gp63 Oncopeltus fasciatus |
| title_short |
Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host |
| title_full |
Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host |
| title_fullStr |
Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host |
| title_full_unstemmed |
Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host |
| title_sort |
Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host |
| author |
Oliveira, Simone Santiago Carvalho de |
| author_facet |
Oliveira, Simone Santiago Carvalho de Gonçalves, Diego de Souza Gomes, Aline dos Santos Garcia Gonçalves, Inês Correa Seabra, Sergio Henrique Menna-Barreto, Rubem Figueiredo Lopes, Angela Hampshire de Carvalho Santos Levy, Claudia Masini d'Avila Santos, André Luis Souza dos Branquinha, Marta Helena |
| author_role |
author |
| author2 |
Gonçalves, Diego de Souza Gomes, Aline dos Santos Garcia Gonçalves, Inês Correa Seabra, Sergio Henrique Menna-Barreto, Rubem Figueiredo Lopes, Angela Hampshire de Carvalho Santos Levy, Claudia Masini d'Avila Santos, André Luis Souza dos Branquinha, Marta Helena |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Simone Santiago Carvalho de Gonçalves, Diego de Souza Gomes, Aline dos Santos Garcia Gonçalves, Inês Correa Seabra, Sergio Henrique Menna-Barreto, Rubem Figueiredo Lopes, Angela Hampshire de Carvalho Santos Levy, Claudia Masini d'Avila Santos, André Luis Souza dos Branquinha, Marta Helena |
| dc.subject.other.pt_BR.fl_str_mv |
Phytomonas serpens Parasitos |
| topic |
Phytomonas serpens Parasitos Phytomonas calpain-like proteins cysteine peptidase cruzipain Gp63 Oncopeltus fasciatus |
| dc.subject.en.pt_BR.fl_str_mv |
Phytomonas calpain-like proteins cysteine peptidase cruzipain Gp63 Oncopeltus fasciatus |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases. Rio de Janeiro, RJ, Brasil. |
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2017 |
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2017-11-16T12:33:03Z |
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2017-11-16T12:33:03Z |
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2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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OLIVEIRA, Simone Santiago Carvalho de; et al. Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host. Mem Inst Oswaldo Cruz, v. 112, n.1, p. 31-43, Jan. 2017. |
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https://www.arca.fiocruz.br/handle/icict/23203 |
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0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/0074-02760160270 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
OLIVEIRA, Simone Santiago Carvalho de; et al. Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host. Mem Inst Oswaldo Cruz, v. 112, n.1, p. 31-43, Jan. 2017. 0074-0276 10.1590/0074-02760160270 1678-8060 |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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