In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42796 |
Resumo: | Brazilian agencies: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo �a Pesquisa do Estado do Amazonas (FAPEAM); and Fundação de Amparo �a Pesquisa do Estado da Bahia (FAPESB). TAA also received scholarship from the Oswaldo Cruz Foundation |
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Anunciação, Talita A. daCosta, Rafaela G. A.Lima, Emilly J. S. P. deSilva, Valdenizia RodriguesSantos, Luciano de S.Soares, Milena Botelho PereiraDias, Rosane BorgesRocha, Clarissa Araújo GurgelCosta, Emmanoel VilaçaSilva, Felipe Moura Araujo daKoolen, Hector Henrique FerreiraBezerra, Daniel Pereira2020-08-17T14:51:23Z2020-08-17T14:51:23Z2020ANUNCIAÇÃO, Talita A. da et al. In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae). Journal of Ethnopharmacology, v. 262, 2020.0378-8741https://www.arca.fiocruz.br/handle/icict/4279610.1016/j.jep.2020.113166Brazilian agencies: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo �a Pesquisa do Estado do Amazonas (FAPEAM); and Fundação de Amparo �a Pesquisa do Estado da Bahia (FAPESB). TAA also received scholarship from the Oswaldo Cruz FoundationFundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry, Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry, Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, BrasiVirola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae), popularly known in Brazil as "mucuíba", "ucuúba", "ucuúba-branca" or "ucuúba do igapó", is a medicinal plant used to treat a variety of diseases, including infections, inflammatory processes and cancer. Aim of the study: In the present work, we investigated the chemical constituents and the in vitro and in vivo inhibition of human colon carcinoma HCT116 cells by essential oils obtained from the bark (EOB) and leaves (EOL) of V. surinamensis. Materials and methods: EOB and EOL were obtained by hydrodistillation and analyzed via gas chromatography with flame ionization detection and gas chromatography coupled to mass spectrometry. In vitro cytotoxic activity was determined in cultured cancer cells HCT116, HepG2, HL-60, B16–F10 and MCF-7 and in a non-cancerous cell line MRC-5 by the Alamar blue assay after 72 h of treatment. Annexin V/propidium iodide staining, mitochondrial transmembrane potential and cell cycle distribution were evaluated by flow cytometry in HCT116 cells treated with essential oils after 24 and 48 h of treatment. The cells were also stained with May-Grunwald-Giemsa to analyze cell morphology. In vivo antitumor activity was evaluated in C.B-17 SCID mice with HCT116 cells. Results: The main constituents in EOB were aristolene (28.0 � 3.1%), α-gurjunene (15.1 � 2.4%), valencene (14.1 � 1.9%), germacrene D (7.5 � 0.9%), δ-guaiene (6.8 � 1.0%) and β-elemene (5.4 � 0.6%). On the other hand, EOL displayed α-farnesene (14.5 � 1.5%), β-elemene (9.6 � 2.3%), bicyclogermacrene (8.1 � 2.0%), germacrene D (7.4 � 0.7%) and α-cubebene (5.6 � 1.1%) as main constituents. EOB showed IC50 values for cancer cells ranging from 9.41 to 29.52 μg/mL for HCT116 and B16–F10, while EOL showed IC50 values for cancer cells ranging from 7.07 to 26.70 μg/mL for HepG2 and HCT116, respectively. The IC50 value for a noncancerous MRC-5 cell was 34.7 and 38.93 μg/mL for EOB and EOL, respectively. Both oils induced apoptotic-like cell death in HCT116 cells, as observed by the morphological characteristics of apoptosis, externalization of phosphatidylserine, mitochondrial depolarization and fragmentation of internucleosomal DNA. At a dose of 40 mg/kg, tumor mass inhibition rates were 57.9 and 44.8% in animals treated with EOB and EOL, respectively. Conclusions: These data indicate V. surinamensis as possible herbal medicine in the treatment of colon cancerengElsevierVirola surinamensisMyristicaceaeMorte celularCelulas HCT116Virola surinamensisMyristicaceaeCell deathHCT116In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. 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| dc.title.pt_BR.fl_str_mv |
In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae) |
| title |
In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae) |
| spellingShingle |
In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae) Anunciação, Talita A. da Virola surinamensis Myristicaceae Morte celular Celulas HCT116 Virola surinamensis Myristicaceae Cell death HCT116 |
| title_short |
In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae) |
| title_full |
In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae) |
| title_fullStr |
In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae) |
| title_full_unstemmed |
In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae) |
| title_sort |
In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae) |
| author |
Anunciação, Talita A. da |
| author_facet |
Anunciação, Talita A. da Costa, Rafaela G. A. Lima, Emilly J. S. P. de Silva, Valdenizia Rodrigues Santos, Luciano de S. Soares, Milena Botelho Pereira Dias, Rosane Borges Rocha, Clarissa Araújo Gurgel Costa, Emmanoel Vilaça Silva, Felipe Moura Araujo da Koolen, Hector Henrique Ferreira Bezerra, Daniel Pereira |
| author_role |
author |
| author2 |
Costa, Rafaela G. A. Lima, Emilly J. S. P. de Silva, Valdenizia Rodrigues Santos, Luciano de S. Soares, Milena Botelho Pereira Dias, Rosane Borges Rocha, Clarissa Araújo Gurgel Costa, Emmanoel Vilaça Silva, Felipe Moura Araujo da Koolen, Hector Henrique Ferreira Bezerra, Daniel Pereira |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Anunciação, Talita A. da Costa, Rafaela G. A. Lima, Emilly J. S. P. de Silva, Valdenizia Rodrigues Santos, Luciano de S. Soares, Milena Botelho Pereira Dias, Rosane Borges Rocha, Clarissa Araújo Gurgel Costa, Emmanoel Vilaça Silva, Felipe Moura Araujo da Koolen, Hector Henrique Ferreira Bezerra, Daniel Pereira |
| dc.subject.other.pt_BR.fl_str_mv |
Virola surinamensis Myristicaceae Morte celular Celulas HCT116 |
| topic |
Virola surinamensis Myristicaceae Morte celular Celulas HCT116 Virola surinamensis Myristicaceae Cell death HCT116 |
| dc.subject.en.pt_BR.fl_str_mv |
Virola surinamensis Myristicaceae Cell death HCT116 |
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Brazilian agencies: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo �a Pesquisa do Estado do Amazonas (FAPEAM); and Fundação de Amparo �a Pesquisa do Estado da Bahia (FAPESB). TAA also received scholarship from the Oswaldo Cruz Foundation |
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2020 |
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2020-08-17T14:51:23Z |
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2020-08-17T14:51:23Z |
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2020 |
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ANUNCIAÇÃO, Talita A. da et al. In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae). Journal of Ethnopharmacology, v. 262, 2020. |
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https://www.arca.fiocruz.br/handle/icict/42796 |
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0378-8741 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.jep.2020.113166 |
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ANUNCIAÇÃO, Talita A. da et al. In vitro and in vivo inhibition of HCT116 cells by essential oils from bark and leaves of Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae). Journal of Ethnopharmacology, v. 262, 2020. 0378-8741 10.1016/j.jep.2020.113166 |
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Elsevier |
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Elsevier |
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