Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11830 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia. Laboratório de Biologia. Duque de Caxias, RJ,Brasil. |
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Amaral, Julio J.Antunes, Luis Caetano M.Macedo, Cristiana S. deMattos, Katherine A.Han, JunPan, JingxiCandéa, André L. P.Henriques, Maria das Graças M. O.Alves, Marcelo RibeiroBorchers, Christoph H.Sarno, Euzenir N.Bozza, Patrícia T.Finlay, B. BrettPessolani, Cristina V.2015-09-28T13:02:36Z2015-09-28T13:02:36Z2013AMARAL, Julio J. et al. Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease. PLoS Neglected Tropical Diseases, v. 7, n. 8, p. 1-15, 2013.https://www.arca.fiocruz.br/handle/icict/1183010.1371/journal.pntd.0002381engPublic Library of ScienceMetabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia. Laboratório de Biologia. Duque de Caxias, RJ,Brasil.The University of British Columbia. Michael Smith Laboratories. Vancouver, British Columbia, Canada / Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.University of Victoria. Genome BC Proteomics Centre. Victoria, British Columbia, Canada.University of Victoria. Genome BC Proteomics Centre. Victoria, British Columbia, Canada.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Farmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Farmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Pesquisa em Farmacogenética. Rio de Janeiro, RJ, Brasil.University of Victoria. Genome BC Proteomics Centre. Victoria, British Columbia, Canada.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.The University of British Columbia. Michael Smith Laboratories. Vancouver, British Columbia, Canada.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Despite considerable efforts over the last decades, our understanding of leprosy pathogenesis remains limited. The complex interplay between pathogens and hosts has profound effects on host metabolism. To explore the metabolic perturbations associated with leprosy, we analyzed the serum metabolome of leprosy patients. Samples collected from lepromatous and tuberculoid patients before and immediately after the conclusion of multidrug therapy (MDT) were subjected to high-throughput metabolic profiling. Our results show marked metabolic alterations during leprosy that subside at the conclusion of MDT. Pathways showing the highest modulation were related to polyunsaturated fatty acid (PUFA) metabolism, with emphasis on anti-inflammatory, pro-resolving omega-3 fatty acids. These results were confirmed by eicosanoid measurements through enzyme-linked immunoassays. Corroborating the repertoire of metabolites altered in sera, metabonomic analysis of skin specimens revealed alterations in the levels of lipids derived from lipase activity, including PUFAs, suggesting a high lipid turnover in highly-infected lesions. Our data suggest that omega-6 and omega-3, PUFA-derived, pro-resolving lipid mediators contribute to reduced tissue damage irrespectively of pathogen burden during leprosy disease. Our results demonstrate the utility of a comprehensive metabonomic approach for identifying potential contributors to disease pathology that may facilitate the development of more targeted treatments for leprosy and other inflammatory diseases.LeprosySerum metabolomePolyunsaturated Fatty AcidsMultidrug therapy (MDT)HanseníaseMetabolomaÁcidos Graxos Insaturadosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11830/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALjulio_amaral_etal_IOC_2013.pdfapplication/pdf607542https://www.arca.fiocruz.br/bitstream/icict/11830/2/julio_amaral_etal_IOC_2013.pdfd633c2251743325feb2cd815cf1dc8dbMD52TEXTjulio_amaral_etal_IOC_2013.pdf.txtjulio_amaral_etal_IOC_2013.pdf.txtExtracted texttext/plain79295https://www.arca.fiocruz.br/bitstream/icict/11830/3/julio_amaral_etal_IOC_2013.pdf.txtf21d7806ebbe4a78f8540497d39fce89MD53icict/118302023-07-28 15:34:42.361oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-28T18:34:42Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease |
| title |
Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease |
| spellingShingle |
Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease Amaral, Julio J. Leprosy Serum metabolome Polyunsaturated Fatty Acids Multidrug therapy (MDT) Hanseníase Metaboloma Ácidos Graxos Insaturados |
| title_short |
Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease |
| title_full |
Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease |
| title_fullStr |
Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease |
| title_full_unstemmed |
Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease |
| title_sort |
Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease |
| author |
Amaral, Julio J. |
| author_facet |
Amaral, Julio J. Antunes, Luis Caetano M. Macedo, Cristiana S. de Mattos, Katherine A. Han, Jun Pan, Jingxi Candéa, André L. P. Henriques, Maria das Graças M. O. Alves, Marcelo Ribeiro Borchers, Christoph H. Sarno, Euzenir N. Bozza, Patrícia T. Finlay, B. Brett Pessolani, Cristina V. |
| author_role |
author |
| author2 |
Antunes, Luis Caetano M. Macedo, Cristiana S. de Mattos, Katherine A. Han, Jun Pan, Jingxi Candéa, André L. P. Henriques, Maria das Graças M. O. Alves, Marcelo Ribeiro Borchers, Christoph H. Sarno, Euzenir N. Bozza, Patrícia T. Finlay, B. Brett Pessolani, Cristina V. |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Amaral, Julio J. Antunes, Luis Caetano M. Macedo, Cristiana S. de Mattos, Katherine A. Han, Jun Pan, Jingxi Candéa, André L. P. Henriques, Maria das Graças M. O. Alves, Marcelo Ribeiro Borchers, Christoph H. Sarno, Euzenir N. Bozza, Patrícia T. Finlay, B. Brett Pessolani, Cristina V. |
| dc.subject.en.pt_BR.fl_str_mv |
Leprosy Serum metabolome Polyunsaturated Fatty Acids Multidrug therapy (MDT) |
| topic |
Leprosy Serum metabolome Polyunsaturated Fatty Acids Multidrug therapy (MDT) Hanseníase Metaboloma Ácidos Graxos Insaturados |
| dc.subject.decs.pt_BR.fl_str_mv |
Hanseníase Metaboloma Ácidos Graxos Insaturados |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia. Laboratório de Biologia. Duque de Caxias, RJ,Brasil. |
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2013 |
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2013 |
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2015-09-28T13:02:36Z |
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2015-09-28T13:02:36Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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AMARAL, Julio J. et al. Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease. PLoS Neglected Tropical Diseases, v. 7, n. 8, p. 1-15, 2013. |
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https://www.arca.fiocruz.br/handle/icict/11830 |
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10.1371/journal.pntd.0002381 |
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AMARAL, Julio J. et al. Metabonomics reveals drastic changes in antiInflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease. PLoS Neglected Tropical Diseases, v. 7, n. 8, p. 1-15, 2013. 10.1371/journal.pntd.0002381 |
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