Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice

Detalhes bibliográficos
Autor(a) principal: Loyo, Rodrigo Moraes
Data de Publicação: 2021
Outros Autores: Zarate, Erica, Barbosa, Constança Simões, Simões-Barbosa, Augusto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57722
Resumo: Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Laboratory and reference service on Schistosomiasis. Recife, PE, Brazil / University of Auckland. School of Biological Sciences. Auckland, New Zealand.
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spelling Loyo, Rodrigo MoraesZarate, EricaBarbosa, Constança SimõesSimões-Barbosa, Augusto2023-04-10T13:17:03Z2023-04-10T13:17:03Z2021LOYO, Rodrigo Moraes et al. Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice. Parasitology International, v. 80, p. 1-4, 2021.1383-5769https://www.arca.fiocruz.br/handle/icict/5772210.1016/j.parint.2020.102239engElsevierGas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleOswaldo Cruz Foundation. Aggeu Magalhães Institute. Laboratory and reference service on Schistosomiasis. Recife, PE, Brazil / University of Auckland. School of Biological Sciences. Auckland, New Zealand.University of Auckland. School of Biological Sciences. Auckland, New Zealand.Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Laboratory and reference service on Schistosomiasis. Recife, PE, Brazil.University of Auckland. School of Biological Sciences. Auckland, New Zealand.Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Laboratório e serviço de referência em EsquistossomoseRecife, PE, Brazil. University of Auckland, School of Biological Sciences, Auckland, New Zealand. University of Auckland, School of Biological Sciences, Auckland, New Zealand.High-throughput profiling of metabolites has been used to identify metabolic changes in murine models as a response to the infection by the parasitic trematode Schistosoma. These investigations have contributed to our understanding on the pathogenesis of this tropical neglected disease, with a potential of helping diagnosis. Here, our study aimed to investigate the application of gas chromatography–mass spectrometry (GC/MS) on the profiling of urine metabolites from mice carrying infections by Schistosoma mansoni. Two larval infection doses created distinctive infection intensities in mice, whereby the heavily infected animals were found to release 25 times more eggs in faeces than lightly infected animals. Over 200 urine metabolites were identified from these animals by GC/MS, following two complementary derivatisation methods. A list of 14 individual metabolites with altered relative abundances between groups were identified. Most of the altered metabolites showed a trend of increased abundances in response to infection intensity, indicating host-specific metabolic alterations as a result of the disease. Hippurate, a metabolite which concentration is intimately modulated by the gut microbiota, was found to be highly correlated to infection intensity. Our study showed that urine metabolic profiling by GC/ MS can distinguish non-infected animals from those carrying light and heavy infections by S. mansoni, revealing metabolites associated to the infection and providing insights on the pathogenesis of schistosomiasis.Schistosoma mansoniGas chromatography–mass spectrometryMetabolomicsMetabolic profilingUrine metaboliteHippurateinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALGas chromatography-mass spectrometry (GCMS) reveals urine metabolites.pdfGas chromatography-mass spectrometry (GCMS) reveals urine metabolites.pdfapplication/pdf1061908https://www.arca.fiocruz.br/bitstream/icict/57722/2/Gas%20chromatography-mass%20spectrometry%20%28GCMS%29%20reveals%20urine%20metabolites.pdf61d3bd40ba2ae4565022ef8f5b644991MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/57722/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/577222023-06-06 16:02:23.123oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-06-06T19:02:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice
title Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice
spellingShingle Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice
Loyo, Rodrigo Moraes
Schistosoma mansoni
Gas chromatography–mass spectrometry
Metabolomics
Metabolic profiling
Urine metabolite
Hippurate
title_short Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice
title_full Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice
title_fullStr Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice
title_full_unstemmed Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice
title_sort Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice
author Loyo, Rodrigo Moraes
author_facet Loyo, Rodrigo Moraes
Zarate, Erica
Barbosa, Constança Simões
Simões-Barbosa, Augusto
author_role author
author2 Zarate, Erica
Barbosa, Constança Simões
Simões-Barbosa, Augusto
author2_role author
author
author
dc.contributor.author.fl_str_mv Loyo, Rodrigo Moraes
Zarate, Erica
Barbosa, Constança Simões
Simões-Barbosa, Augusto
dc.subject.en.none.fl_str_mv Schistosoma mansoni
Gas chromatography–mass spectrometry
Metabolomics
Metabolic profiling
Urine metabolite
Hippurate
topic Schistosoma mansoni
Gas chromatography–mass spectrometry
Metabolomics
Metabolic profiling
Urine metabolite
Hippurate
description Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Laboratory and reference service on Schistosomiasis. Recife, PE, Brazil / University of Auckland. School of Biological Sciences. Auckland, New Zealand.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2023-04-10T13:17:03Z
dc.date.available.fl_str_mv 2023-04-10T13:17:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LOYO, Rodrigo Moraes et al. Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice. Parasitology International, v. 80, p. 1-4, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57722
dc.identifier.issn.none.fl_str_mv 1383-5769
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.parint.2020.102239
identifier_str_mv LOYO, Rodrigo Moraes et al. Gas chromatography-mass spectrometry (GC/MS) reveals urine metabolites associated to light and heavy infections by Schistosoma mansoni in mice. Parasitology International, v. 80, p. 1-4, 2021.
1383-5769
10.1016/j.parint.2020.102239
url https://www.arca.fiocruz.br/handle/icict/57722
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/57722/2/Gas%20chromatography-mass%20spectrometry%20%28GCMS%29%20reveals%20urine%20metabolites.pdf
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