A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/20492 |
Resumo: | Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Laboratório de Diversidade Microbiana da Amazônia com Importância para a Saúde. Manaus, AM, Brasil. |
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Aquino, Priscila Ferreira deCarvalho, Paulo CostaNogueira, Fábio Cesar SousaFonseca, Clóvis Orlando daSilva, Júlio Cesar Thomé de SouzaCarvalho, Maria da Gloria da CostaDomont, Gilberto B.Zanchin, Nilson Ivo ToninFischer, Juliana de Saldanha da Gama2017-08-02T15:12:19Z2017-08-02T15:12:19Z2016AQUINO Priscila F. de et. al. A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme. Frontiers in Oncology, v. 6, n. 183, p. 1-10, 2016.2234-943Xhttps://www.arca.fiocruz.br/handle/icict/2049210.3389/fonc.2016.00183porFrontiers MediaHeterogeneidade MolecularGlioblastomaGenetic HeterogeneityProteomicsGlioblastomaHeterogeneidad GenéticaProteómicaGlioblastomaHeterogeneidade GenéticaProteômicaA Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiformeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Laboratório de Diversidade Microbiana da Amazônia com Importância para a Saúde. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Laboratory for Protein Chemistry, Chemistry Institute. Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil.Department of General and Specialized Surgery. Antonio Pedro University Hospital. Fluminense Federal University. Rio de Janeiro, RJ, Brazil.Department of Neurosurgery. Ipanema Federal Hospital. Rio de Janeiro, RJ, Brazil.Laboratory of Molecular Pathology. Department of Pathology. University Hospital Clementino Fraga Filho. Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil.Laboratory for Protein Chemistry, Chemistry Institute. Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Tumors consist of cells in different stages of transformation with molecular and cellular heterogeneity. By far, heterogeneity is the hallmark of glioblastoma multiforme (GBM), the most malignant and aggressive type of glioma. Most proteomic studies aim in comparing tumors from different patients, but here we dive into exploring the intratumoral proteome diversity of a single GBM. For this, we profiled tumor fragments from the profound region of the same patient’s GBM but obtained from two surgeries a year’s time apart. Our analysis also included GBM‘s fragments from different anatomical regions. Our quantitative proteomic strategy employed 4-plex iTRAQ peptide labeling followed by a four-step strong cation chromatographic separation; each fraction was then analyzed by reversed-phase nano-chromatography coupled on-line with an Orbitrap-Velos mass spectrometer. Unsupervised clustering grouped the proteomic profiles into four major distinct groups and showed that most changes were related to the tumor’s anatomical region. Nevertheless, we report differentially abundant proteins from GBM’s fragments of the same region but obtained 1 year apart. We discuss several key proteins (e.g., S100A9) and enriched pathways linked with GBM such as the Ras pathway, RHO GTPases activate PKNs, and those related to apoptosis, to name a few. As far as we know, this is the only report that compares GBM fragments proteomic profiles from the same patient. Ultimately, our results fuel the forefront of scientific discussion on the importance in exploring the richness of subproteomes within a single tissue sample for a better understanding of the disease, as each tumor is unique.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/20492/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALA TIME-BASED AND INTRATUMORAL PROTEOMIC ASSESSMENT OF A RECURRENT GLIOBLASTOMA MULTIFORME.pdfA TIME-BASED AND INTRATUMORAL PROTEOMIC ASSESSMENT OF A RECURRENT GLIOBLASTOMA MULTIFORME.pdfapplication/pdf2275179https://www.arca.fiocruz.br/bitstream/icict/20492/2/A%20TIME-BASED%20AND%20INTRATUMORAL%20PROTEOMIC%20ASSESSMENT%20OF%20A%20RECURRENT%20GLIOBLASTOMA%20MULTIFORME.pdf120ed17193feb1c0a2bfbac97875b076MD52TEXTA TIME-BASED AND INTRATUMORAL PROTEOMIC ASSESSMENT OF A 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| dc.title.pt_BR.fl_str_mv |
A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme |
| title |
A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme |
| spellingShingle |
A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme Aquino, Priscila Ferreira de Heterogeneidade Molecular Glioblastoma Genetic Heterogeneity Proteomics Glioblastoma Heterogeneidad Genética Proteómica Glioblastoma Heterogeneidade Genética Proteômica |
| title_short |
A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme |
| title_full |
A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme |
| title_fullStr |
A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme |
| title_full_unstemmed |
A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme |
| title_sort |
A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme |
| author |
Aquino, Priscila Ferreira de |
| author_facet |
Aquino, Priscila Ferreira de Carvalho, Paulo Costa Nogueira, Fábio Cesar Sousa Fonseca, Clóvis Orlando da Silva, Júlio Cesar Thomé de Souza Carvalho, Maria da Gloria da Costa Domont, Gilberto B. Zanchin, Nilson Ivo Tonin Fischer, Juliana de Saldanha da Gama |
| author_role |
author |
| author2 |
Carvalho, Paulo Costa Nogueira, Fábio Cesar Sousa Fonseca, Clóvis Orlando da Silva, Júlio Cesar Thomé de Souza Carvalho, Maria da Gloria da Costa Domont, Gilberto B. Zanchin, Nilson Ivo Tonin Fischer, Juliana de Saldanha da Gama |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Aquino, Priscila Ferreira de Carvalho, Paulo Costa Nogueira, Fábio Cesar Sousa Fonseca, Clóvis Orlando da Silva, Júlio Cesar Thomé de Souza Carvalho, Maria da Gloria da Costa Domont, Gilberto B. Zanchin, Nilson Ivo Tonin Fischer, Juliana de Saldanha da Gama |
| dc.subject.other.pt_BR.fl_str_mv |
Heterogeneidade Molecular |
| topic |
Heterogeneidade Molecular Glioblastoma Genetic Heterogeneity Proteomics Glioblastoma Heterogeneidad Genética Proteómica Glioblastoma Heterogeneidade Genética Proteômica |
| dc.subject.en.pt_BR.fl_str_mv |
Glioblastoma Genetic Heterogeneity Proteomics |
| dc.subject.es.pt_BR.fl_str_mv |
Glioblastoma Heterogeneidad Genética Proteómica |
| dc.subject.decs.pt_BR.fl_str_mv |
Glioblastoma Heterogeneidade Genética Proteômica |
| description |
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Laboratório de Diversidade Microbiana da Amazônia com Importância para a Saúde. Manaus, AM, Brasil. |
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2016 |
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2016 |
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2017-08-02T15:12:19Z |
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2017-08-02T15:12:19Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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AQUINO Priscila F. de et. al. A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme. Frontiers in Oncology, v. 6, n. 183, p. 1-10, 2016. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/20492 |
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2234-943X |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.3389/fonc.2016.00183 |
| identifier_str_mv |
AQUINO Priscila F. de et. al. A Time-Based and Intratumoral Proteomic Assessment of a Recurrent Glioblastoma Multiforme. Frontiers in Oncology, v. 6, n. 183, p. 1-10, 2016. 2234-943X 10.3389/fonc.2016.00183 |
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Frontiers Media |
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Frontiers Media |
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