Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/30198 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (473667/2012-6 and 311054/2014-5), Fundação de Amparo à Pesquisa do Estado da Bahia - FAPESB (2574/2013), and Fundação Nacional para o Desenvolvimento do Ensino Superior - FUNDADESP (9600113). |
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Brito, Vanessa da SilvaSantos, Fred Luciano NevesGonçalves, Noilson Lazaro SousaAraujo, Thessika Hialla AlmeidaNascimento, Davi Silva ValePereira, Felicidade MotaBoa-Sorte, Ney Cristian AmaralGrassi, Maria Fernanda RiosAraujo, Adele Caterino deCastro Filho, Bernardo Galvão2018-11-26T16:45:17Z2018-11-26T16:45:17Z2018BRITO, Vanessa da Silva et al. Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil. Journal of Clinical Microbiology, p. 1-26, Sept. 2018.https://www.arca.fiocruz.br/handle/icict/3019810.1128/JCM.00961-18Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (473667/2012-6 and 311054/2014-5), Fundação de Amparo à Pesquisa do Estado da Bahia - FAPESB (2574/2013), and Fundação Nacional para o Desenvolvimento do Ensino Superior - FUNDADESP (9600113).Bahiana School of Medicine and Public Health. Integrated and Multidisciplinary HTLV Center. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Bahiana School of Medicine and Public Health. Integrated and Multidisciplinary HTLV Center. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Bahiana School of Medicine and Public Health. Integrated and Multidisciplinary HTLV Center. Salvador, BA, Brazil.Bahiana School of Medicine and Public Health. Integrated and Multidisciplinary HTLV Center. Salvador, BA, Brazil.Gonçalo Moniz Public Health Central Laboratory. Bahia State Department of Health. Salvador, BA, Brazil.Bahiana School of Medicine and Public Health. Integrated and Multidisciplinary HTLV Center. Salvador, BA, Brazil.Bahiana School of Medicine and Public Health. Integrated and Multidisciplinary HTLV Center. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Adolfo Lutz Institute. São Paulo, SP, Brazil.Bahiana School of Medicine and Public Health. Integrated and Multidisciplinary HTLV Center. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Serological screening for HTLV-1 is usually performed using enzyme-linked immunosorbent assay, particle agglutination or chemiluminescence assay kits. Due to antigen matrix improvement entailing the use of new HTLV-antigens and changes in the format of HTLV screening tests, as well as newly introduced CLIAs, a systematic evaluation of the accuracy of currently available commercial tests is warranted. We aimed to assess the performance of commercially available screening tests for HTLV diagnosis. A diagnostic accuracy study was conducted on a panel of 397 plasma samples: 200 HTLV-negative, 170 HTLV-positive and 27 indeterminate under Western blotting analysis. WB-indeterminate samples (i.e. those yielding no specific bands for HTLV-1 and/or HTLV-2) were assessed by PCR and results were used to compare agreement among the commercially available ELISA screening tests. For performance analysis, WB-indeterminate samples were excluded, resulting in a final study panel of 370 samples. Three ELISA kits (Murex HTLV-1/2, anti-HTLV-1/2 SYM Solution and Gold ELISA HTLV-1/2) and one CLIA kit (Architect r-HTLV-1/2) were evaluated. All screening tests demonstrated 100% sensitivity. Concerning the HTLV-negative samples, SYM Solution and Gold ELISA kits had specificity values >99.5%, while the Architect r-HTLV-1/2 test presented 98.1% specificity, followed by Murex (92.0%). Regarding the 27 samples with WB-indeterminate results, after PCR confirmation, all ELISA kits showed 100% sensitivity, but low specificity. Accuracy findings were corroborated by Cohen's Kappa, which evidenced slight and fair agreement between PCR analysis and ELISA tests for HTLV diagnosis. Based on the data, we believe that all evaluated tests can be safely used for HTLV-infection screening.engAmerican Society for MicrobiologyPerformance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHTLVScreening testsDiagnostic reagent kitsNãoNão Publicadoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/30198/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBrito VS Performance of screening...2018.pdfBrito VS Performance of screening...2018.pdfapplication/pdf2236052https://www.arca.fiocruz.br/bitstream/icict/30198/2/Brito%20VS%20Performance%20of%20screening...2018.pdf3a37b8b65aceafdc9b2b7c985c4249a9MD52TEXTBrito VS Performance of screening...2018.pdf.txtBrito VS Performance of screening...2018.pdf.txtExtracted texttext/plain43048https://www.arca.fiocruz.br/bitstream/icict/30198/3/Brito%20VS%20Performance%20of%20screening...2018.pdf.txtfcd784b441248bc32f8f3ca86a98bf60MD53icict/301982019-07-10 23:04:19.387oai:www.arca.fiocruz.br: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ório 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| dc.title.pt_BR.fl_str_mv |
Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil |
| title |
Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil |
| spellingShingle |
Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil Brito, Vanessa da Silva HTLV Screening tests Diagnostic reagent kits |
| title_short |
Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil |
| title_full |
Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil |
| title_fullStr |
Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil |
| title_full_unstemmed |
Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil |
| title_sort |
Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil |
| author |
Brito, Vanessa da Silva |
| author_facet |
Brito, Vanessa da Silva Santos, Fred Luciano Neves Gonçalves, Noilson Lazaro Sousa Araujo, Thessika Hialla Almeida Nascimento, Davi Silva Vale Pereira, Felicidade Mota Boa-Sorte, Ney Cristian Amaral Grassi, Maria Fernanda Rios Araujo, Adele Caterino de Castro Filho, Bernardo Galvão |
| author_role |
author |
| author2 |
Santos, Fred Luciano Neves Gonçalves, Noilson Lazaro Sousa Araujo, Thessika Hialla Almeida Nascimento, Davi Silva Vale Pereira, Felicidade Mota Boa-Sorte, Ney Cristian Amaral Grassi, Maria Fernanda Rios Araujo, Adele Caterino de Castro Filho, Bernardo Galvão |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Brito, Vanessa da Silva Santos, Fred Luciano Neves Gonçalves, Noilson Lazaro Sousa Araujo, Thessika Hialla Almeida Nascimento, Davi Silva Vale Pereira, Felicidade Mota Boa-Sorte, Ney Cristian Amaral Grassi, Maria Fernanda Rios Araujo, Adele Caterino de Castro Filho, Bernardo Galvão |
| dc.subject.en.pt_BR.fl_str_mv |
HTLV Screening tests Diagnostic reagent kits |
| topic |
HTLV Screening tests Diagnostic reagent kits |
| description |
Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (473667/2012-6 and 311054/2014-5), Fundação de Amparo à Pesquisa do Estado da Bahia - FAPESB (2574/2013), and Fundação Nacional para o Desenvolvimento do Ensino Superior - FUNDADESP (9600113). |
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2018 |
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2018-11-26T16:45:17Z |
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2018-11-26T16:45:17Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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BRITO, Vanessa da Silva et al. Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil. Journal of Clinical Microbiology, p. 1-26, Sept. 2018. |
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https://www.arca.fiocruz.br/handle/icict/30198 |
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10.1128/JCM.00961-18 |
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BRITO, Vanessa da Silva et al. Performance of commercially available serological screening tests for human T-cell lymphotropic virus infection in Brazil. Journal of Clinical Microbiology, p. 1-26, Sept. 2018. 10.1128/JCM.00961-18 |
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American Society for Microbiology |
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American Society for Microbiology |
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