Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46741 |
Resumo: | Universidade Federal do Maranhão. Rede Nordeste de Biotecnologia. São Luis, MA, Brasil. |
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Mondêgo-Oliveira, RenataSouza, Joyce Cortez de SáMoragas-Tellis, Carla JunqueiraSouza, Paulo Victor Ramos deChagas, Maria do Socorro dos SantosBehrens, Maria DutraHardoim, Daiana de JesusTaniwaki, Noemi NosomiChometon, Thaize QuirogaBerho, Alvaro LuizCalabrese, Kátia da SilvaSouza, Fernando AlmeidaSilva, Ana Lucia Abreu2021-04-16T18:32:23Z2021-04-16T18:32:23Z2021MONDÊGO-OLIVEIRA, Renata et al. Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes. Biomedicine & Pharmacology, v. 133, 111025, p. 1-12, 2021.0753-3322https://www.arca.fiocruz.br/handle/icict/4674110.1016/j.biopha.2020.111025engElsevierβ-caryophylleneCélulas DH82Citometria de fluxoIsobologramaLeishmanioseMicroscopia eletrônica de transmissãoβ-caryophylleneDH82 cellsFlow cytometryIsobologramLeishmaniasisTransmission electron microscopyVernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Maranhão. Rede Nordeste de Biotecnologia. São Luis, MA, Brasil.Universidade CEUMA. São Luis, MA, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Produtos Naturais para Saúde Pública. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Tecnologia em Fármacos. Laboratório de Produtos Naturais para Saúde Pública. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Tecnologia em Fármacos. Programa de Pós-Graduação Acadêmico em Pesquisa Translacional em Fármacos e Medicamentos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Produtos Naturais para Saúde Pública. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Produtos Naturais para Saúde Pública. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Instituto Adolfo Lutz. Núcleo de Microscopia Eletrônica. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma Tecnológica de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade Estadual do Maranhao. São Luis, MA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade Estadual do Maranhão. São Luís, MA, Brasil.The present study aimed to evaluate the antileishmanial effect, the mechanisms of action and the association with miltefosine of Vernonia brasiliana essential oil against Leishmania infantum promastigotes. This essential oil was obtained by hydrodistillation and its chemical composition was determined by gas chromatography-mass spectrometry (GC–MS). The antileishmanial activity against L. infantum promastigotes and cytotoxicity on DH82 cells were evaluated by MTT colorimetric assay. Ultrastructural alterations were evaluated by transmission electron microscopy. Changes in mitochondrial membrane potential, in the production of reactive oxygen spe cies, and analysis of apoptotic events were determined by flow cytometry. The association between the essential oil and miltefosine was evaluated using the modified isobologram method. The most abundant component of the essential oil was β-caryophyllene (21.47 %). Anti-Leishmania assays indicated an IC50 of 39.01 ± 1.080 μg/mL for promastigote forms after 72 h of treatment. The cytotoxic concentration for DH82 cells was 63.13 ± 1.211 μg/mL after 24 h of treatment. The effect against L. infantum was proven through the ultrastructural changes caused by the oil, such as kinetoplast and mitochondrial swelling, vesicles in the flagellar pocket, discontinuity of the nuclear membrane, nuclear fragmentation and condensation, and loss of organelles. It was observed that the oil leads to a decrease in the mitochondrial membrane potential (35.10 %, p = 0.0031), increased reactive oxygen species production, and cell death by late apoptosis (17.60 %, p = 0.020). The combination of the essential oil and miltefosine exhibited an antagonistic effect. This study evidences the antileishmanial action of V. brasiliana essential oil against L. infantum promastigotes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46741/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAlvaroBertho_DayanaHardoim_etal_IOC_2012.pdfAlvaroBertho_DayanaHardoim_etal_IOC_2012.pdfapplication/pdf5488042https://www.arca.fiocruz.br/bitstream/icict/46741/2/AlvaroBertho_DayanaHardoim_etal_IOC_2012.pdfed57a0950c0409604565f03f536086c2MD52TEXTAlvaroBertho_DayanaHardoim_etal_IOC_2012.pdf.txtAlvaroBertho_DayanaHardoim_etal_IOC_2012.pdf.txtExtracted texttext/plain72741https://www.arca.fiocruz.br/bitstream/icict/46741/3/AlvaroBertho_DayanaHardoim_etal_IOC_2012.pdf.txt0355348c5226034fa7ffe37c99e31a17MD53icict/467412022-06-24 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| dc.title.pt_BR.fl_str_mv |
Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes |
| title |
Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes |
| spellingShingle |
Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes Mondêgo-Oliveira, Renata β-caryophyllene Células DH82 Citometria de fluxo Isobolograma Leishmaniose Microscopia eletrônica de transmissão β-caryophyllene DH82 cells Flow cytometry Isobologram Leishmaniasis Transmission electron microscopy |
| title_short |
Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes |
| title_full |
Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes |
| title_fullStr |
Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes |
| title_full_unstemmed |
Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes |
| title_sort |
Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes |
| author |
Mondêgo-Oliveira, Renata |
| author_facet |
Mondêgo-Oliveira, Renata Souza, Joyce Cortez de Sá Moragas-Tellis, Carla Junqueira Souza, Paulo Victor Ramos de Chagas, Maria do Socorro dos Santos Behrens, Maria Dutra Hardoim, Daiana de Jesus Taniwaki, Noemi Nosomi Chometon, Thaize Quiroga Berho, Alvaro Luiz Calabrese, Kátia da Silva Souza, Fernando Almeida Silva, Ana Lucia Abreu |
| author_role |
author |
| author2 |
Souza, Joyce Cortez de Sá Moragas-Tellis, Carla Junqueira Souza, Paulo Victor Ramos de Chagas, Maria do Socorro dos Santos Behrens, Maria Dutra Hardoim, Daiana de Jesus Taniwaki, Noemi Nosomi Chometon, Thaize Quiroga Berho, Alvaro Luiz Calabrese, Kátia da Silva Souza, Fernando Almeida Silva, Ana Lucia Abreu |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mondêgo-Oliveira, Renata Souza, Joyce Cortez de Sá Moragas-Tellis, Carla Junqueira Souza, Paulo Victor Ramos de Chagas, Maria do Socorro dos Santos Behrens, Maria Dutra Hardoim, Daiana de Jesus Taniwaki, Noemi Nosomi Chometon, Thaize Quiroga Berho, Alvaro Luiz Calabrese, Kátia da Silva Souza, Fernando Almeida Silva, Ana Lucia Abreu |
| dc.subject.other.pt_BR.fl_str_mv |
β-caryophyllene Células DH82 Citometria de fluxo Isobolograma Leishmaniose Microscopia eletrônica de transmissão |
| topic |
β-caryophyllene Células DH82 Citometria de fluxo Isobolograma Leishmaniose Microscopia eletrônica de transmissão β-caryophyllene DH82 cells Flow cytometry Isobologram Leishmaniasis Transmission electron microscopy |
| dc.subject.en.pt_BR.fl_str_mv |
β-caryophyllene DH82 cells Flow cytometry Isobologram Leishmaniasis Transmission electron microscopy |
| description |
Universidade Federal do Maranhão. Rede Nordeste de Biotecnologia. São Luis, MA, Brasil. |
| publishDate |
2021 |
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2021-04-16T18:32:23Z |
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2021-04-16T18:32:23Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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MONDÊGO-OLIVEIRA, Renata et al. Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes. Biomedicine & Pharmacology, v. 133, 111025, p. 1-12, 2021. |
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https://www.arca.fiocruz.br/handle/icict/46741 |
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0753-3322 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.biopha.2020.111025 |
| identifier_str_mv |
MONDÊGO-OLIVEIRA, Renata et al. Vernonia brasiliana (L.) Druce induces ultrastructural changes and apoptosis-like death of Leishmania infantum promastigotes. Biomedicine & Pharmacology, v. 133, 111025, p. 1-12, 2021. 0753-3322 10.1016/j.biopha.2020.111025 |
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Elsevier |
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Elsevier |
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