Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/50487 |
Resumo: | 1 Instituto Gonçalo Moniz, FIOCRUZ Bahia, Fundação Oswaldo Cruz / FIOCRUZ, Rua Waldemar Falcão, n. 121, Candeal, Salvador, Bahia 40296710, Brazil 2 Universidade Católica do Salvador (UCSAL), Salvador, Bahia, Brazil 3 Fundação Oswaldo Cruz, Instituto Carlos Chagas (ICC-FIOCRUZ/PR), Curitiba, Paraná, Brazil 4 Universidade Federal do Oeste da Bahia (UFOB), Barreiras, Bahia, Brazil 5 Universidade Federal da Bahia (UFBA), Salvador, Bahia, Brazil 6 Universidade Estadual de Santa Cruz (UESC), Ilhéus, Bahia, Brazil 7 Fundação de Hematologia e Hemoterapia da Bahia (HEMOBA), Salvador, Bahia, Brazil 8 Hospital Universitário Professor Edgard Santos (HUPES), UFBA, Salvador, Bahia, Brazil |
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Pitanga, Thassila N.Santana, Sânzio S.Zanette, Dalila L.Guarda, Caroline C.Santiago, Rayra P.Maffili, Vitor V.Lima, Jonilson B.Carvalho, Graziele Q.Ribeiro Filho, JaimeFerreira, Junia R. D.Aleluia, Milena M.Nascimento, Valma M. L.Carvalho, Magda O. S.Lyra, Isa M.Borges, Valéria de MatosOliveira, Ricardo R.Gonçalves, Marilda de Sousa2021-12-20T14:33:07Z2021-12-20T14:33:07Z2021PITANGA, Thassila N. et al.Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells. Inflammation Research, 2021.1023-3830https://www.arca.fiocruz.br/handle/icict/5048710.1007/s00011-021-01461-21 Instituto Gonçalo Moniz, FIOCRUZ Bahia, Fundação Oswaldo Cruz / FIOCRUZ, Rua Waldemar Falcão, n. 121, Candeal, Salvador, Bahia 40296710, Brazil 2 Universidade Católica do Salvador (UCSAL), Salvador, Bahia, Brazil 3 Fundação Oswaldo Cruz, Instituto Carlos Chagas (ICC-FIOCRUZ/PR), Curitiba, Paraná, Brazil 4 Universidade Federal do Oeste da Bahia (UFOB), Barreiras, Bahia, Brazil 5 Universidade Federal da Bahia (UFBA), Salvador, Bahia, Brazil 6 Universidade Estadual de Santa Cruz (UESC), Ilhéus, Bahia, Brazil 7 Fundação de Hematologia e Hemoterapia da Bahia (HEMOBA), Salvador, Bahia, Brazil 8 Hospital Universitário Professor Edgard Santos (HUPES), UFBA, Salvador, Bahia, BrazilTNP received a scholarship from CAPES. V.M.B. and MSG have productivity scholarship from CNPq. This work was supported by grants from the Brazilian National Council of Research (CNPq) (311888/2013-5) (M.S.G.); the Foundation of Research and Extension of Bahia (FAPESB) (3626/2013, 1431040053063, and 9073/2007) (M.S.G.); and PPSUS/FAPESB (020/2013 EFP00007295), (M.S.G.); the Instituto Nacional de Ciência e Tecnologia do Sangue (INCTs) (Coordinated by S.T.O.S.), and MCD/CNPq/MS-SCTIE-DECIT (409800/2006-6), (M.S.G.)."Múltipla ver em Notas"Objective and design This study tested the hypothesis that sickle red blood cell (SS-RBC) can induce inflammasome NLRP3 components gene expression in peripheral blood mononuclear cells (PBMCs) as well as interleukin-1β (IL-1β) and leukotriene B4 (LTB4) production. Additionally, we investigated the effect of hydroxyurea (HU) treatment in these inflammatory markers. Methods PBMCs from healthy donors (AA-PBMC) were challenged with intact and lysed RBCs from SCA patients (SSRBC) and from healthy volunteers (AA-RBC). NLRP3, IL-1β, IL-18 and Caspase-1 gene expression levels were assessed by quantitative PCR (qPCR). IL-1β protein levels and LTB4 were measured by ELISA. Results We observed that lysed SS-RBC induced the expression of inflammasome NLRP3 components, but this increase was more prominent for CASP1 and IL18 expression levels. Moreover, we observed that intact SS-RBC induced higher production of IL-1β and LTB4 than lysed SS-RBC. Although SCA patients treated with HU have a reduction in NLRP3 gene expression and LTB4 production, this treatment did not modulate the expression of other inflammasome components or IL-1β production. Conclusions Thus, our data suggest that caspase-1, IL-1β and IL-18 may contribute to the inflammatory status observed in SCA and that HU treatment may not interfere in this inflammatory pathway.engSpringerAnemia falciformeInflamassomaHemóliseHidroxiureiaHemeSickle cell anemiaNLRP3-inflammasomeHemolysisHydroxyureaHemeEffect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/50487/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALPitanga, Thassila N. Effect....pdfPitanga, Thassila N. Effect....pdfapplication/pdf2194187https://www.arca.fiocruz.br/bitstream/icict/50487/2/Pitanga%2c%20Thassila%20N.%20Effect....pdf9a78615c9c2c90a8e3f58d419e3fe922MD52icict/504872023-03-15 14:34:33.929oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells |
| title |
Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells |
| spellingShingle |
Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells Pitanga, Thassila N. Anemia falciforme Inflamassoma Hemólise Hidroxiureia Heme Sickle cell anemia NLRP3-inflammasome Hemolysis Hydroxyurea Heme |
| title_short |
Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells |
| title_full |
Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells |
| title_fullStr |
Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells |
| title_full_unstemmed |
Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells |
| title_sort |
Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells |
| author |
Pitanga, Thassila N. |
| author_facet |
Pitanga, Thassila N. Santana, Sânzio S. Zanette, Dalila L. Guarda, Caroline C. Santiago, Rayra P. Maffili, Vitor V. Lima, Jonilson B. Carvalho, Graziele Q. Ribeiro Filho, Jaime Ferreira, Junia R. D. Aleluia, Milena M. Nascimento, Valma M. L. Carvalho, Magda O. S. Lyra, Isa M. Borges, Valéria de Matos Oliveira, Ricardo R. Gonçalves, Marilda de Sousa |
| author_role |
author |
| author2 |
Santana, Sânzio S. Zanette, Dalila L. Guarda, Caroline C. Santiago, Rayra P. Maffili, Vitor V. Lima, Jonilson B. Carvalho, Graziele Q. Ribeiro Filho, Jaime Ferreira, Junia R. D. Aleluia, Milena M. Nascimento, Valma M. L. Carvalho, Magda O. S. Lyra, Isa M. Borges, Valéria de Matos Oliveira, Ricardo R. Gonçalves, Marilda de Sousa |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pitanga, Thassila N. Santana, Sânzio S. Zanette, Dalila L. Guarda, Caroline C. Santiago, Rayra P. Maffili, Vitor V. Lima, Jonilson B. Carvalho, Graziele Q. Ribeiro Filho, Jaime Ferreira, Junia R. D. Aleluia, Milena M. Nascimento, Valma M. L. Carvalho, Magda O. S. Lyra, Isa M. Borges, Valéria de Matos Oliveira, Ricardo R. Gonçalves, Marilda de Sousa |
| dc.subject.other.pt_BR.fl_str_mv |
Anemia falciforme Inflamassoma Hemólise Hidroxiureia Heme |
| topic |
Anemia falciforme Inflamassoma Hemólise Hidroxiureia Heme Sickle cell anemia NLRP3-inflammasome Hemolysis Hydroxyurea Heme |
| dc.subject.en.pt_BR.fl_str_mv |
Sickle cell anemia NLRP3-inflammasome Hemolysis Hydroxyurea Heme |
| description |
1 Instituto Gonçalo Moniz, FIOCRUZ Bahia, Fundação Oswaldo Cruz / FIOCRUZ, Rua Waldemar Falcão, n. 121, Candeal, Salvador, Bahia 40296710, Brazil 2 Universidade Católica do Salvador (UCSAL), Salvador, Bahia, Brazil 3 Fundação Oswaldo Cruz, Instituto Carlos Chagas (ICC-FIOCRUZ/PR), Curitiba, Paraná, Brazil 4 Universidade Federal do Oeste da Bahia (UFOB), Barreiras, Bahia, Brazil 5 Universidade Federal da Bahia (UFBA), Salvador, Bahia, Brazil 6 Universidade Estadual de Santa Cruz (UESC), Ilhéus, Bahia, Brazil 7 Fundação de Hematologia e Hemoterapia da Bahia (HEMOBA), Salvador, Bahia, Brazil 8 Hospital Universitário Professor Edgard Santos (HUPES), UFBA, Salvador, Bahia, Brazil |
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2021 |
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2021-12-20T14:33:07Z |
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2021-12-20T14:33:07Z |
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2021 |
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PITANGA, Thassila N. et al.Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells. Inflammation Research, 2021. |
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https://www.arca.fiocruz.br/handle/icict/50487 |
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1023-3830 |
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10.1007/s00011-021-01461-2 |
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PITANGA, Thassila N. et al.Effect of lysed and non‑lysed sickle red cells on the activation of NLRP3 inflammasome and LTB4 production by mononuclear cells. Inflammation Research, 2021. 1023-3830 10.1007/s00011-021-01461-2 |
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