Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections

Detalhes bibliográficos
Autor(a) principal: Amaral, V. F.
Data de Publicação: 2001
Outros Autores: Teva, A., Porrozi, R., Silva, A. J., Pereira, M. S., Oliveira Neto, M. P., Grimaldi, G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33450
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
id CRUZ_9900ad3adcc9256fe86c3f956467a3b2
oai_identifier_str oai:www.arca.fiocruz.br:icict/33450
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Amaral, V. F.Teva, A.Porrozi, R.Silva, A. J.Pereira, M. S.Oliveira Neto, M. P.Grimaldi, G.2019-06-11T12:18:29Z2019-06-11T12:18:29Z2001AMARAL, V. F. et al. Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 6, p. 795-804, 2001.0074-0276https://www.arca.fiocruz.br/handle/icict/3345010.1590/S0074-027620010006000101678-8060engInstituto Oswaldo CruzLeishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infectionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Hospital Evandro Chagas. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Seven rhesus macaques were infected intradermally with 107 promastigotes of Leishmania (Leishmania) major. All monkeys developed a localized, ulcerative, self-healing nodular skin lesion at the site of inoculation of the parasite. Non-specific chronic inflammation and/or tuberculoid-type granulomatous reaction were the main histopathological manifestations of the disease. Serum Leishmania-specific antibodies (IgG and IgG1) were detected by ELISA in all infected animals; immunoblot analyses indicated that numerous antigens were recognized. A very high degree of variability was observed in the parasite-specific cell-mediated immune responses [as detected by measuring delayed-type hypersensitivity (DTH) reaction, in vitro lymphocyte proliferation, and gamma interferon (IFN-γ) production] for individuals over time post challenge. From all the recovered monkeys (which showed resolution of the lesions after 11 weeks of infection), 57.2% (4/7) and 28.6% (2/7) animals remained susceptible to secondary and tertiary infections, respectively, but the disease severity was altered (i.e. lesion size was smaller and healed faster than in the primary infection). The remaining monkeys exhibited complete resistance (i.e. no lesion) to each rechallenge. Despite the inability to consistently detect correlates of cell-mediated immunity to Leishmania or correlation between resistance to challenge and DTH, lymphocyte transformation or IFN-γ production, partial or complete acquired resistance was conferred by experimental infection. This primate model should be useful for measuring vaccine effectiveness against the human disease.Rhesus macaquesMacaca mulattaNonhuman primatesExperimental leishmaniasisLeishmania (L.) majorImmune responsesHistopathologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33450/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Amaral_V_etal_INI_2001.pdfve_Amaral_V_etal_INI_2001.pdfapplication/pdf125899https://www.arca.fiocruz.br/bitstream/icict/33450/2/ve_Amaral_V_etal_INI_2001.pdf1465e96de0824dbf8454896dba8d8dc9MD52TEXTve_Amaral_V_etal_INI_2001.pdf.txtve_Amaral_V_etal_INI_2001.pdf.txtExtracted texttext/plain47764https://www.arca.fiocruz.br/bitstream/icict/33450/3/ve_Amaral_V_etal_INI_2001.pdf.txt5e991b65807213316797f5b65ca86ca7MD53icict/334502019-06-12 02:00:53.85oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-12T05:00:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections
title Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections
spellingShingle Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections
Amaral, V. F.
Rhesus macaques
Macaca mulatta
Nonhuman primates
Experimental leishmaniasis
Leishmania (L.) major
Immune responses
Histopathology
title_short Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections
title_full Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections
title_fullStr Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections
title_full_unstemmed Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections
title_sort Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections
author Amaral, V. F.
author_facet Amaral, V. F.
Teva, A.
Porrozi, R.
Silva, A. J.
Pereira, M. S.
Oliveira Neto, M. P.
Grimaldi, G.
author_role author
author2 Teva, A.
Porrozi, R.
Silva, A. J.
Pereira, M. S.
Oliveira Neto, M. P.
Grimaldi, G.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Amaral, V. F.
Teva, A.
Porrozi, R.
Silva, A. J.
Pereira, M. S.
Oliveira Neto, M. P.
Grimaldi, G.
dc.subject.en.pt_BR.fl_str_mv Rhesus macaques
Macaca mulatta
Nonhuman primates
Experimental leishmaniasis
Leishmania (L.) major
Immune responses
Histopathology
topic Rhesus macaques
Macaca mulatta
Nonhuman primates
Experimental leishmaniasis
Leishmania (L.) major
Immune responses
Histopathology
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2019-06-11T12:18:29Z
dc.date.available.fl_str_mv 2019-06-11T12:18:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AMARAL, V. F. et al. Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 6, p. 795-804, 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33450
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762001000600010
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv AMARAL, V. F. et al. Leishmania (Leishmania) major-infected Rhesus macaques (Macaca mulatta) develop varying levels of resistance against homologous re-infections. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 6, p. 795-804, 2001.
0074-0276
10.1590/S0074-02762001000600010
1678-8060
url https://www.arca.fiocruz.br/handle/icict/33450
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
publisher.none.fl_str_mv Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33450/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/33450/2/ve_Amaral_V_etal_INI_2001.pdf
https://www.arca.fiocruz.br/bitstream/icict/33450/3/ve_Amaral_V_etal_INI_2001.pdf.txt
bitstream.checksum.fl_str_mv 8a4605be74aa9ea9d79846c1fba20a33
1465e96de0824dbf8454896dba8d8dc9
5e991b65807213316797f5b65ca86ca7
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009110688333824

Registros relacionados