Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico

Detalhes bibliográficos
Autor(a) principal: Anez, German
Data de Publicação: 2016
Outros Autores: Rios, Maria, Heisey, Daniel A. R., Chancey, Caren, Fares, Rafaelle C. G., Espina, Luz M., Souza, Kátia P. R., Carvalho, Andréa Teixeira de, Krysztof, David E., Foster, Gregory A., Stramer, Susan L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59512
Resumo: U.S. Food and Drug Administration. Silver Spring, MA, United States of America/Sanofi Pasteur. Swiftwater, PE, United States of America
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spelling Anez, GermanRios, MariaHeisey, Daniel A. R.Chancey, CarenFares, Rafaelle C. G.Espina, Luz M.Souza, Kátia P. R.Carvalho, Andréa Teixeira deKrysztof, David E.Foster, Gregory A.Stramer, Susan L.Rios, Maria2023-07-11T17:51:40Z2023-07-11T17:51:40Z2016AÑEZ, Germán et al. Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico. PLoS Negl Trop Dis., v. 10, n. 2, e0004445, 2016. doi: 10.1371/journal.pntd.0004445.1935-2735https://www.arca.fiocruz.br/handle/icict/5951210.1371/journal.pntd.0004445engPublic Library of ScienceDistribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Ricoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleU.S. Food and Drug Administration. Silver Spring, MA, United States of America/Sanofi Pasteur. Swiftwater, PE, United States of AmericaU.S. Food and Drug Administration. Silver Spring, MA, United States of America/Virginia Commonwealth University. Richmond, Virginia, United States of AmericaU.S. Food and Drug Administration. Silver Spring, MA, United States of AmericaU.S. Food and Drug Administration. Silver Spring, MA, United States of AmericaU.S. Food and Drug Administration. Silver Spring, MA, United States of America/Universidad del Zulia. Maracaibo, VenezuelaU.S. Food and Drug Administration. Silver Spring, MA, United States of America/ Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrazilU.S. Food and Drug Administration. Silver Spring, MA, United States of America/ Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou, Belo Horizonte, MG, BrazilAmerican Red Cross. Gaithersburg, MA, United States of AmericaAmerican Red Cross. Gaithersburg, MA, United States of AmericaAmerican Red Cross. Gaithersburg, MA, United States of AmericaU.S. Food and Drug Administration. Silver Spring, MA, United States of AmericaBackground Dengue is a mosquito-borne viral disease caused by the four dengue viruses (DENV-1 to 4) that can also be transmitted by blood transfusion and organ transplantation. The distribution of DENV in the components of blood from infected donors is poorly understood. Methods We used an in-house TaqMan qRT-PCR assay to test residual samples of plasma, cellular components of whole blood (CCWB), serum and clot specimens from the same collection from blood donors who were DENV-RNA-reactive in a parallel blood safety study. To assess whether DENV RNA detected by TaqMan was associated with infectious virus, DENV infectivity in available samples was determined by culture in mosquito cells. Results DENV RNA was detected by TaqMan in all tested blood components, albeit more consistently in the cellular components; 78.8% of CCWB, 73.3% of clots, 86.7% of sera and 41.8% of plasma samples. DENV-1 was detected in 48 plasma and 97 CCWB samples while DENV-4 was detected in 21 plasma and 31 CCWB samples. In mosquito cell cultures, 29/111 (26.1%) plasma and 32/97 (32.7%) CCWB samples were infectious. A subset of samples from 29 donors was separately analyzed to compare DENV viral loads in the available blood components. DENV viral loads did not differ significantly between components and ranged from 3-8 log(10) PCR-detectable units/ml. Conclusions DENV was present in all tested components from most donors, and viral RNA was not preferentially distributed in any of the tested components. Infectious DENV was also present in similar proportions in cultured plasma, clot and CCWB samples, indicating that these components may serve as a resource when sample sizes are limited. However, these results suggest that the sensitivity of the nucleic acid tests (NAT) for these viruses would not be improved by testing whole blood or components other than plasmainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALDistribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico.pdfDistribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico.pdfapplication/pdf468949https://www.arca.fiocruz.br/bitstream/icict/59512/2/Distribution%20of%20Dengue%20Virus%20Types%201%20and%204%20in%20Blood%20Components%20from%20Infected%20Blood%20Donors%20from%20Puerto%20Rico.pdf65665e9698eaf88909dd9696a3f58413MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59512/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/595122023-07-11 14:51:41.716oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-11T17:51:41Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico
title Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico
spellingShingle Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico
Anez, German
title_short Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico
title_full Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico
title_fullStr Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico
title_full_unstemmed Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico
title_sort Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico
author Anez, German
author_facet Anez, German
Rios, Maria
Heisey, Daniel A. R.
Chancey, Caren
Fares, Rafaelle C. G.
Espina, Luz M.
Souza, Kátia P. R.
Carvalho, Andréa Teixeira de
Krysztof, David E.
Foster, Gregory A.
Stramer, Susan L.
author_role author
author2 Rios, Maria
Heisey, Daniel A. R.
Chancey, Caren
Fares, Rafaelle C. G.
Espina, Luz M.
Souza, Kátia P. R.
Carvalho, Andréa Teixeira de
Krysztof, David E.
Foster, Gregory A.
Stramer, Susan L.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Anez, German
Rios, Maria
Heisey, Daniel A. R.
Chancey, Caren
Fares, Rafaelle C. G.
Espina, Luz M.
Souza, Kátia P. R.
Carvalho, Andréa Teixeira de
Krysztof, David E.
Foster, Gregory A.
Stramer, Susan L.
Rios, Maria
description U.S. Food and Drug Administration. Silver Spring, MA, United States of America/Sanofi Pasteur. Swiftwater, PE, United States of America
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2023-07-11T17:51:40Z
dc.date.available.fl_str_mv 2023-07-11T17:51:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv AÑEZ, Germán et al. Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico. PLoS Negl Trop Dis., v. 10, n. 2, e0004445, 2016. doi: 10.1371/journal.pntd.0004445.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59512
dc.identifier.issn.en_US.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0004445
identifier_str_mv AÑEZ, Germán et al. Distribution of Dengue Virus Types 1 and 4 in Blood Components from Infected Blood Donors from Puerto Rico. PLoS Negl Trop Dis., v. 10, n. 2, e0004445, 2016. doi: 10.1371/journal.pntd.0004445.
1935-2735
10.1371/journal.pntd.0004445
url https://www.arca.fiocruz.br/handle/icict/59512
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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