A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics

Detalhes bibliográficos
Autor(a) principal: Lavich, T. R.
Data de Publicação: 2005
Outros Autores: Cordeiro, R. S. B., Silva, P. M. R., Martins, M. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/37746
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
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spelling Lavich, T. R.Cordeiro, R. S. B.Silva, P. M. R.Martins, M. A.2019-12-10T13:38:51Z2019-12-10T13:38:51Z2005LAVICH, T. R. et al. A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 38, p. 445-451, 2005.0100-879Xhttps://www.arca.fiocruz.br/handle/icict/3774610.1590/s0100-879x20050003000161414-431XengAssociação Brasileira de Divulgação CientíficaHyperalgesiaTeste de HargreavesTeste modificado da placa quenteCarrageninaProstaglandina E2IndometacinaHyperalgesiaHargreaves’ testModified hot-plate testCarrageenanProstaglandin E2IndomethacinA novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesicsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.It is widely accepted that the classical constant-temperature hot-plate test is insensitive to cyclooxygenase inhibitors. In the current study, we developed a variant of the hot-plate test procedure (modified hot-plate (MHP) test) to measure inflammatory nociception in freely moving rats and mice. Following left and right hind paw stimulation with a phlogogen and vehicle, respectively, the animals were placed individually on a hot-plate surface at 51 degrees C and the withdrawal latency for each paw was determined simultaneously in measurements performed at 15, 60, 180, and 360 min post-challenge. Plantar stimulation of rats (250 and 500 microg/paw) and mice (125-500 microg/paw) with carrageenan led to a rapid hyperalgesic response of the ipsilateral paw that reached a plateau from 15 to 360 min after challenge. Pretreatment with indomethacin (4 mg/kg, i.p.) inhibited the phenomenon at all the times analyzed. Similarly, plantar stimulation of rats and mice with prostaglandin E2 (0.5 and 1 microg/paw) also resulted in rapid hyperalgesia which was first detected 15 min post-challenge. Finally, we observed that the MHP test was more sensitive than the classical Hargreaves' test, being able to detect about 4- and 10-fold lower doses of prostaglandin E2 and carrageenan, respectively. In conclusion, the MHP test is a simple and sensitive method for detecting peripheral hyperalgesia and analgesia in rats and mice. This test represents a low-cost alternative for the study of inflammatory pain in freely moving animals.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/37746/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarcoMartins_PRMSilva_etal_IOC_2005.pdfMarcoMartins_PRMSilva_etal_IOC_2005.pdfapplication/pdf512088https://www.arca.fiocruz.br/bitstream/icict/37746/2/MarcoMartins_PRMSilva_etal_IOC_2005.pdf6c39667d5c0afc91435501eb0a07a543MD52TEXTMarcoMartins_PRMSilva_etal_IOC_2005.pdf.txtMarcoMartins_PRMSilva_etal_IOC_2005.pdf.txtExtracted texttext/plain24188https://www.arca.fiocruz.br/bitstream/icict/37746/3/MarcoMartins_PRMSilva_etal_IOC_2005.pdf.txt48b9ab5cc8169287d7690f6ba074c18aMD53icict/377462019-12-11 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dc.title.pt_BR.fl_str_mv A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics
title A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics
spellingShingle A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics
Lavich, T. R.
Hyperalgesia
Teste de Hargreaves
Teste modificado da placa quente
Carragenina
Prostaglandina E2
Indometacina
Hyperalgesia
Hargreaves’ test
Modified hot-plate test
Carrageenan
Prostaglandin E2
Indomethacin
title_short A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics
title_full A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics
title_fullStr A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics
title_full_unstemmed A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics
title_sort A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics
author Lavich, T. R.
author_facet Lavich, T. R.
Cordeiro, R. S. B.
Silva, P. M. R.
Martins, M. A.
author_role author
author2 Cordeiro, R. S. B.
Silva, P. M. R.
Martins, M. A.
author2_role author
author
author
dc.contributor.author.fl_str_mv Lavich, T. R.
Cordeiro, R. S. B.
Silva, P. M. R.
Martins, M. A.
dc.subject.other.pt_BR.fl_str_mv Hyperalgesia
Teste de Hargreaves
Teste modificado da placa quente
Carragenina
Prostaglandina E2
Indometacina
topic Hyperalgesia
Teste de Hargreaves
Teste modificado da placa quente
Carragenina
Prostaglandina E2
Indometacina
Hyperalgesia
Hargreaves’ test
Modified hot-plate test
Carrageenan
Prostaglandin E2
Indomethacin
dc.subject.en.pt_BR.fl_str_mv Hyperalgesia
Hargreaves’ test
Modified hot-plate test
Carrageenan
Prostaglandin E2
Indomethacin
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2019-12-10T13:38:51Z
dc.date.available.fl_str_mv 2019-12-10T13:38:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LAVICH, T. R. et al. A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 38, p. 445-451, 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/37746
dc.identifier.issn.pt_BR.fl_str_mv 0100-879X
dc.identifier.doi.none.fl_str_mv 10.1590/s0100-879x2005000300016
dc.identifier.eissn.none.fl_str_mv 1414-431X
identifier_str_mv LAVICH, T. R. et al. A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 38, p. 445-451, 2005.
0100-879X
10.1590/s0100-879x2005000300016
1414-431X
url https://www.arca.fiocruz.br/handle/icict/37746
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/37746/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/37746/2/MarcoMartins_PRMSilva_etal_IOC_2005.pdf
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