Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys

Detalhes bibliográficos
Autor(a) principal: Marchevsky, Renato S.
Data de Publicação: 2003
Outros Autores: Freire, Marcos S., Coutinho, Evandro S. F., Galler, Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32236
Resumo: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
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spelling Marchevsky, Renato S.Freire, Marcos S.Coutinho, Evandro S. F.Galler, Ricardo2019-03-26T14:17:17Z2019-03-26T14:17:17Z2003MARCHEVSKY, Renato S. et al. Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys. Virology, New York, v. 316, n. 1, p.55-63, 2003.1096-0341https://www.arca.fiocruz.br/handle/icict/3223610.1016/S0042-6822(03)00583-XengNeurovirulence of yellow fever 17DD vaccine virus to rhesus monkeysinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.The yellow fever 17D virus is attenuated and used for human vaccination. Two of its substrains, 17D-204 and 17DD, are used for vaccine production. One of the remarkable properties of this vaccine is limited viral replication in the host but with significant dissemination of the viral mass, yielding a robust and long-lived neutralizing antibody response. The vaccine has excellent records of efficacy and safety and is cheap, used as a single dose, and there are well-established production methodology and quality control procedures which include the monkey neurovirulence test (MNTV). The present study aims at a better understanding of YF 17DD virus attenuation and immunogenicity in the MNVT with special emphasis on viremia, seroconversion, clinical and histological lesions scores, and their intrinsic variability across the tests. Several MNVTs were performed using the secondary seed lot virus 17DD 102/84 totaling 49 rhesus monkeys. Viremia was never higher than the accepted limits established in international requirements, and high levels of neutralizing antibodies were observed in all animals. None of the animals showed visceral lesions. We found that the clinical scores for the same virus varied widely across the tests. There was a direct correlation between the clinical scores in animals with clinical signs of encephalitis and a higher degree of central nervous system (CNS) histological lesions, with an increase of lesions in areas of the CNS such as the substantia nigra, nucleus caudatus, intumescentia cervicalis, and intumescentia ventralis. The histological scores were shown to be less prone to individual variations and had a more homogeneous value distribution among the tests. Since 17DD 102/84 seed virus has been used for human vaccine production and immunization for 16 years with the vaccine being safe and efficacious, it demonstrates that the observed variations across the MNVTs do not influence its effect on humans.Yellow fever vaccine17DD vaccine virusMonkey neurovirulence testinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/32236/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALF110-1-s2.0-S004268220300583X-main.pdfapplication/pdf430970https://www.arca.fiocruz.br/bitstream/icict/32236/2/F110-1-s2.0-S004268220300583X-main.pdf5bfbcbb7e109012a39842e968f627180MD52TEXTF110-1-s2.0-S004268220300583X-main.pdf.txtF110-1-s2.0-S004268220300583X-main.pdf.txtExtracted texttext/plain37481https://www.arca.fiocruz.br/bitstream/icict/32236/3/F110-1-s2.0-S004268220300583X-main.pdf.txt394b32c9128738167d1662a2c830e358MD53icict/322362023-09-05 10:30:37.3oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:30:37Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys
title Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys
spellingShingle Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys
Marchevsky, Renato S.
Yellow fever vaccine
17DD vaccine virus
Monkey neurovirulence test
title_short Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys
title_full Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys
title_fullStr Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys
title_full_unstemmed Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys
title_sort Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys
author Marchevsky, Renato S.
author_facet Marchevsky, Renato S.
Freire, Marcos S.
Coutinho, Evandro S. F.
Galler, Ricardo
author_role author
author2 Freire, Marcos S.
Coutinho, Evandro S. F.
Galler, Ricardo
author2_role author
author
author
dc.contributor.author.fl_str_mv Marchevsky, Renato S.
Freire, Marcos S.
Coutinho, Evandro S. F.
Galler, Ricardo
dc.subject.en.pt_BR.fl_str_mv Yellow fever vaccine
17DD vaccine virus
Monkey neurovirulence test
topic Yellow fever vaccine
17DD vaccine virus
Monkey neurovirulence test
description Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2019-03-26T14:17:17Z
dc.date.available.fl_str_mv 2019-03-26T14:17:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv MARCHEVSKY, Renato S. et al. Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys. Virology, New York, v. 316, n. 1, p.55-63, 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32236
dc.identifier.issn.pt_BR.fl_str_mv 1096-0341
dc.identifier.doi.none.fl_str_mv 10.1016/S0042-6822(03)00583-X
identifier_str_mv MARCHEVSKY, Renato S. et al. Neurovirulence of yellow fever 17DD vaccine virus to rhesus monkeys. Virology, New York, v. 316, n. 1, p.55-63, 2003.
1096-0341
10.1016/S0042-6822(03)00583-X
url https://www.arca.fiocruz.br/handle/icict/32236
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