Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/54810 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brasil. |
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Honorato, LeandroAraujo, Joana Feital Demetrio deEllis, Cameron C.Piffer, Alicia CorbelliniPereira, YanFrases, SusanaAraújo, Glauber Ribeiro de SousaPontes, BrunoMendes, Maria TaysPereira, Marcos DiasGuimarães, Allan J.Silva, Natalia Martins daVargas, GabrieleJoffe, Lunadel Poeta, MaurizioNosanchuk, Joshua D.Zamith-Miranda, DanielReis, Flávia Coelho Garcia dosOliveira, Haroldo Cesar deRodrigues, Marcio LourençoMartins, Sharon de ToledoAlves, Lysangela RonalteAlmeida, Igor C.Nimrichter, Leonardo2022-09-15T18:56:05Z2022-09-15T18:56:05Z2022HONORATO, Leandro et al. Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans. MBio, v. 13, n. 3, p. 1–21, 2022.2150-7511https://www.arca.fiocruz.br/handle/icict/5481010.1128/mbio.00301-22porAmerican Society for MicrobiologyExtracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brasil.Department of Biological Sciences. Border Biomedical Research Center. University of Texas El Paso. El Paso, Texas, USA.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas e Centro Nacional de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Department of Biological Sciences. Border Biomedical Research Center. University of Texas El Paso. El Paso, Texas, USA.Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Laboratório de Citotoxicidade e Genotoxicidade. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Laboratório de Bioquímica e Imunologia das Micoses. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brasil.Department of Microbiology and Immunology. Stony Brook University. Stony Brook. New York, USA.Department of Microbiology and Immunology. Stony Brook University. Stony Brook. New York, USA. / Department of Microbiology and Immunology and Division of Infectious Diseases. Stony Brook University. Stony Brook, New York, USA. / Veterans Affairs Medical Center. Northport. New York, USA.Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, New York, USA. / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, New York, USA.Department of Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, New York, USA. / Division of Infectious Diseases. Department of Medicine. Albert Einstein College of Medicine. Bronx, New York, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Department of Biological Sciences. Border Biomedical Research Center. University of Texas El Paso. El Paso, Texas, USA.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia Geral. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brasil.In this study, we investigated the influence of fungal extracellular vesicles (EVs) during biofilm formation and morphogenesis in Candida albicans. Using crystal violet staining and scanning electron microscopy (SEM), we demonstrated that C. albicans EVs inhibited biofilm formation in vitro. By time-lapse microscopy and SEM, we showed that C. albicans EV treatment stopped filamentation and promoted pseudohyphae formation with multiple budding sites. The ability of C. albicans EVs to regulate dimorphism was further compared to EVs isolated from different C. albicans strains, Saccharomyces cerevisiae, and Histoplasma capsulatum. C. albicans EVs from distinct strains inhibited yeast-to-hyphae differentiation with morphological changes occurring in less than 4 h. EVs from S. cerevisiae and H. capsulatum modestly reduced morphogenesis, and the effect was evident after 24 h of incubation. The inhibitory activity of C. albicans EVs on phase transition was promoted by a combination of lipid compounds, which were identified by gas chromatography-tandem mass spectrometry analysis as sesquiterpenes, diterpenes, and fatty acids. Remarkably, C. albicans EVs were also able to reverse filamentation. Finally, C. albicans cells treated with C. albicans EVs for 24 h lost their capacity to penetrate agar and were avirulent when inoculated into Galleria mellonella. Our results indicate that fungal EVs can regulate yeast-to-hypha differentiation, thereby inhibiting biofilm formation and attenuating virulence.Extracellular VesiclesLipidsBiofilmsHyphaeVesículas ExtracelularesLípidosBiopelículasHifaCandida albicansVesículas ExtracelularesLipídeosBiofilmesHifasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/54810/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALmbio.00301-22ok.pdfmbio.00301-22ok.pdfapplication/pdf3093476https://www.arca.fiocruz.br/bitstream/icict/54810/2/mbio.00301-22ok.pdf8b9fd4add1655788a696395b028241ecMD52icict/548102022-09-19 15:14:40.148oai:www.arca.fiocruz.br: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ório 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| dc.title.en_US.fl_str_mv |
Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans |
| title |
Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans |
| spellingShingle |
Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans Honorato, Leandro Extracellular Vesicles Lipids Biofilms Hyphae Vesículas Extracelulares Lípidos Biopelículas Hifa Candida albicans Vesículas Extracelulares Lipídeos Biofilmes Hifas |
| title_short |
Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans |
| title_full |
Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans |
| title_fullStr |
Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans |
| title_full_unstemmed |
Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans |
| title_sort |
Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans |
| author |
Honorato, Leandro |
| author_facet |
Honorato, Leandro Araujo, Joana Feital Demetrio de Ellis, Cameron C. Piffer, Alicia Corbellini Pereira, Yan Frases, Susana Araújo, Glauber Ribeiro de Sousa Pontes, Bruno Mendes, Maria Tays Pereira, Marcos Dias Guimarães, Allan J. Silva, Natalia Martins da Vargas, Gabriele Joffe, Luna del Poeta, Maurizio Nosanchuk, Joshua D. Zamith-Miranda, Daniel Reis, Flávia Coelho Garcia dos Oliveira, Haroldo Cesar de Rodrigues, Marcio Lourenço Martins, Sharon de Toledo Alves, Lysangela Ronalte Almeida, Igor C. Nimrichter, Leonardo |
| author_role |
author |
| author2 |
Araujo, Joana Feital Demetrio de Ellis, Cameron C. Piffer, Alicia Corbellini Pereira, Yan Frases, Susana Araújo, Glauber Ribeiro de Sousa Pontes, Bruno Mendes, Maria Tays Pereira, Marcos Dias Guimarães, Allan J. Silva, Natalia Martins da Vargas, Gabriele Joffe, Luna del Poeta, Maurizio Nosanchuk, Joshua D. Zamith-Miranda, Daniel Reis, Flávia Coelho Garcia dos Oliveira, Haroldo Cesar de Rodrigues, Marcio Lourenço Martins, Sharon de Toledo Alves, Lysangela Ronalte Almeida, Igor C. Nimrichter, Leonardo |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Honorato, Leandro Araujo, Joana Feital Demetrio de Ellis, Cameron C. Piffer, Alicia Corbellini Pereira, Yan Frases, Susana Araújo, Glauber Ribeiro de Sousa Pontes, Bruno Mendes, Maria Tays Pereira, Marcos Dias Guimarães, Allan J. Silva, Natalia Martins da Vargas, Gabriele Joffe, Luna del Poeta, Maurizio Nosanchuk, Joshua D. Zamith-Miranda, Daniel Reis, Flávia Coelho Garcia dos Oliveira, Haroldo Cesar de Rodrigues, Marcio Lourenço Martins, Sharon de Toledo Alves, Lysangela Ronalte Almeida, Igor C. Nimrichter, Leonardo |
| dc.subject.en.en_US.fl_str_mv |
Extracellular Vesicles Lipids Biofilms Hyphae |
| topic |
Extracellular Vesicles Lipids Biofilms Hyphae Vesículas Extracelulares Lípidos Biopelículas Hifa Candida albicans Vesículas Extracelulares Lipídeos Biofilmes Hifas |
| dc.subject.es.en_US.fl_str_mv |
Vesículas Extracelulares Lípidos Biopelículas Hifa |
| dc.subject.decs.en_US.fl_str_mv |
Candida albicans Vesículas Extracelulares Lipídeos Biofilmes Hifas |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Microbiologia. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brasil. |
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2022 |
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2022-09-15T18:56:05Z |
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2022-09-15T18:56:05Z |
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2022 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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HONORATO, Leandro et al. Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans. MBio, v. 13, n. 3, p. 1–21, 2022. |
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https://www.arca.fiocruz.br/handle/icict/54810 |
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2150-7511 |
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10.1128/mbio.00301-22 |
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HONORATO, Leandro et al. Extracellular vesicles regulate biofilm formation and yeast-to- hypha differentiation in Candida albicans. MBio, v. 13, n. 3, p. 1–21, 2022. 2150-7511 10.1128/mbio.00301-22 |
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American Society for Microbiology |
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American Society for Microbiology |
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