Molecular characterisation of methicillinresistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil

Detalhes bibliográficos
Autor(a) principal: Rodrigues, D. C. S.
Data de Publicação: 2020
Outros Autores: Lima, D. F., Cohen, R. W. F., Marques, E. A., Leão, R. S.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44369
Resumo: Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
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spelling Rodrigues, D. C. S.Lima, D. F.Cohen, R. W. F.Marques, E. A.Leão, R. S.2020-11-10T18:47:44Z2020-11-10T18:47:44Z2020RODRIGUES, D. C. S. et al. Molecular characterisation of methicillin-resistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil. Epidemiology and Infection, p. 1-7, May 2020.0950-2688https://www.arca.fiocruz.br/handle/icict/4436910.1017/S0950268820001156porCambridge University PressBrazil / epidemiologyCarrier StateChildCystic Fibrosis / microbiologyHumansMethicillin ResistanceMethicillin-Resistant Staphylococcus aureus / geneticsStaphylococcal Infections / epidemiologyStaphylococcal Infections / microbiologyCystic fibrosisSpa typingMRSAPFGESCCmecStaphylococcus aureusMolecular characterisation of methicillinresistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional da Saúde da Mulher da Criança e do Adolescente Fernandes Figueira. Departamento de Pneumologia Pediátrica. Rio de Janeiro, RJ, BrasilUniversidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.Persistent methicillin-resistant Staphylococcus aureus (MRSA) infection in cystic fibrosis (CF) patients has been associated with a more rapid decline in lung function, increased hospitalisation and mortality. The aim of this study was to evaluate the clonal relationships among 116 MRSA isolates from 12 chronically colonised CF pediatric patients over a 6-year period in a Rio de Janeiro CF specialist centre. Isolates were characterised by antimicrobial resistance, SCCmec type, presence of Panton-Valentine Leukocidin (PVL) genes and grouped according to DNA macrorestriction profile by pulsed-field gel electrophoresis (PFGE) and spa gene type. High resistance rates were detected for erythromycin (78%) and ciprofloxacin (50%) and SCCmec IV was the most common type (72.4%). Only 8.6% of isolates were PVL positive. High genetic diversity was evident by PFGE (39 pulsotypes) and of nine that were identified spa types, t002 (53.1%) and t539 (14.8%) were the most prevalent. We conclude that the observed homogeneity of spa types within patients over the study period demonstrates the persistence of such strain lineages throughout the course of chronic lung infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83134https://www.arca.fiocruz.br/bitstream/icict/44369/1/license.txt0ab46789d568c4ba27c7b5d29a9fe9c4MD51ORIGINALRodrigues_D. C. S_etal_IFF_2020.pdfRodrigues_D. C. S_etal_IFF_2020.pdfapplication/pdf250804https://www.arca.fiocruz.br/bitstream/icict/44369/2/Rodrigues_D.%20C.%20S_etal_IFF_2020.pdf9f87e8d8b8776f891f7e516912970278MD52TEXTRodrigues_D. C. S_etal_IFF_2020.pdf.txtRodrigues_D. C. 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dc.title.pt_BR.fl_str_mv Molecular characterisation of methicillinresistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil
title Molecular characterisation of methicillinresistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil
spellingShingle Molecular characterisation of methicillinresistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil
Rodrigues, D. C. S.
Brazil / epidemiology
Carrier State
Child
Cystic Fibrosis / microbiology
Humans
Methicillin Resistance
Methicillin-Resistant Staphylococcus aureus / genetics
Staphylococcal Infections / epidemiology
Staphylococcal Infections / microbiology
Cystic fibrosis
Spa typing
MRSA
PFGE
SCCmec
Staphylococcus aureus
title_short Molecular characterisation of methicillinresistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil
title_full Molecular characterisation of methicillinresistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil
title_fullStr Molecular characterisation of methicillinresistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil
title_full_unstemmed Molecular characterisation of methicillinresistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil
title_sort Molecular characterisation of methicillinresistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil
author Rodrigues, D. C. S.
author_facet Rodrigues, D. C. S.
Lima, D. F.
Cohen, R. W. F.
Marques, E. A.
Leão, R. S.
author_role author
author2 Lima, D. F.
Cohen, R. W. F.
Marques, E. A.
Leão, R. S.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues, D. C. S.
Lima, D. F.
Cohen, R. W. F.
Marques, E. A.
Leão, R. S.
dc.subject.mesh.pt_BR.fl_str_mv Brazil / epidemiology
Carrier State
Child
Cystic Fibrosis / microbiology
Humans
Methicillin Resistance
Methicillin-Resistant Staphylococcus aureus / genetics
Staphylococcal Infections / epidemiology
Staphylococcal Infections / microbiology
topic Brazil / epidemiology
Carrier State
Child
Cystic Fibrosis / microbiology
Humans
Methicillin Resistance
Methicillin-Resistant Staphylococcus aureus / genetics
Staphylococcal Infections / epidemiology
Staphylococcal Infections / microbiology
Cystic fibrosis
Spa typing
MRSA
PFGE
SCCmec
Staphylococcus aureus
dc.subject.en.pt_BR.fl_str_mv Cystic fibrosis
Spa typing
MRSA
PFGE
SCCmec
Staphylococcus aureus
description Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-11-10T18:47:44Z
dc.date.available.fl_str_mv 2020-11-10T18:47:44Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RODRIGUES, D. C. S. et al. Molecular characterisation of methicillin-resistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil. Epidemiology and Infection, p. 1-7, May 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44369
dc.identifier.issn.pt_BR.fl_str_mv 0950-2688
dc.identifier.doi.none.fl_str_mv 10.1017/S0950268820001156
identifier_str_mv RODRIGUES, D. C. S. et al. Molecular characterisation of methicillin-resistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil. Epidemiology and Infection, p. 1-7, May 2020.
0950-2688
10.1017/S0950268820001156
url https://www.arca.fiocruz.br/handle/icict/44369
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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