Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40087 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
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Cardoso, JosianeLima, Carla de PaulaLeal, TiagoGradia, Daniela FioriFragoso, Stenio PerdigãoGoldenberg, SamuelSá, Renata Guerra deKrieger, Marco Aurélio2020-02-19T15:10:52Z2020-02-19T15:10:52Z2011CARDOSO, Josiane et al. Analysis of Proteasomal Proteolysis during the In Vitro Metacyclogenesis of Trypanosoma cruzi. Plos One, v. 6, n. 6, p. 1-9, 2011.1932-6203https://www.arca.fiocruz.br/handle/icict/4008710.1371/journal.pone.00210271932-6203porPublic Library of ScienceTrypanosoma cruziProteasome Endopeptidase ComplexProteolysisUbiquitinComplejo de la Endopetidasa ProteasomalProteolisisUbiquitinaComplexo de Endopeptidases do ProteassomaProteóliseUbiquitinaAnalysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal de Ouro Preto. Ouro Preto, Minas Gerais, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal de Ouro Preto. Ouro Preto, Minas Gerais, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Proteasomes are large protein complexes, whose main function is to degrade unnecessary or damaged proteins. The inhibition of proteasome activity in Trypanosoma cruzi blocks parasite replication and cellular differentiation. We demonstrate that proteasome-dependent proteolysis occurs during the cellular differentiation of T. cruzi from replicative non-infectious epimastigotes to non-replicative and infectious trypomastigotes (metacyclogenesis). No peaks of ubiquitin-mediated degradation were observed and the profile of ubiquitinated conjugates was similar at all stages of differentiation. However, an analysis of carbonylated proteins showed significant variation in oxidized protein levels at the various stages of differentiation and the proteasome inhibition also increased oxidized protein levels. Our data suggest that different proteasome complexes coexist during metacyclogenesis. The 20S proteasome may be free or linked to regulatory particles (PA700, PA26 and PA200), at specific cell sites and the coordinated action of these complexes would make it possible for proteolysis of ubiquitin-tagged proteins and oxidized proteins, to coexist in the cell.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/40087/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALjournal.pone.0021027.PDFjournal.pone.0021027.PDFapplication/pdf1377266https://www.arca.fiocruz.br/bitstream/icict/40087/2/journal.pone.0021027.PDF707e64d697a3a40a1e8e011008b85f85MD52TEXTjournal.pone.0021027.PDF.txtjournal.pone.0021027.PDF.txtExtracted texttext/plain50240https://www.arca.fiocruz.br/bitstream/icict/40087/3/journal.pone.0021027.PDF.txt604681dc35389e08ccf258c5f562e846MD53icict/400872020-02-20 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| dc.title.pt_BR.fl_str_mv |
Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi |
| title |
Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi |
| spellingShingle |
Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi Cardoso, Josiane Trypanosoma cruzi Proteasome Endopeptidase Complex Proteolysis Ubiquitin Complejo de la Endopetidasa Proteasomal Proteolisis Ubiquitina Complexo de Endopeptidases do Proteassoma Proteólise Ubiquitina |
| title_short |
Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi |
| title_full |
Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi |
| title_fullStr |
Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi |
| title_full_unstemmed |
Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi |
| title_sort |
Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi |
| author |
Cardoso, Josiane |
| author_facet |
Cardoso, Josiane Lima, Carla de Paula Leal, Tiago Gradia, Daniela Fiori Fragoso, Stenio Perdigão Goldenberg, Samuel Sá, Renata Guerra de Krieger, Marco Aurélio |
| author_role |
author |
| author2 |
Lima, Carla de Paula Leal, Tiago Gradia, Daniela Fiori Fragoso, Stenio Perdigão Goldenberg, Samuel Sá, Renata Guerra de Krieger, Marco Aurélio |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cardoso, Josiane Lima, Carla de Paula Leal, Tiago Gradia, Daniela Fiori Fragoso, Stenio Perdigão Goldenberg, Samuel Sá, Renata Guerra de Krieger, Marco Aurélio |
| dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi |
| topic |
Trypanosoma cruzi Proteasome Endopeptidase Complex Proteolysis Ubiquitin Complejo de la Endopetidasa Proteasomal Proteolisis Ubiquitina Complexo de Endopeptidases do Proteassoma Proteólise Ubiquitina |
| dc.subject.en.pt_BR.fl_str_mv |
Proteasome Endopeptidase Complex Proteolysis Ubiquitin |
| dc.subject.es.pt_BR.fl_str_mv |
Complejo de la Endopetidasa Proteasomal Proteolisis Ubiquitina |
| dc.subject.decs.pt_BR.fl_str_mv |
Complexo de Endopeptidases do Proteassoma Proteólise Ubiquitina |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
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2011 |
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2011 |
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2020-02-19T15:10:52Z |
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2020-02-19T15:10:52Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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CARDOSO, Josiane et al. Analysis of Proteasomal Proteolysis during the In Vitro Metacyclogenesis of Trypanosoma cruzi. Plos One, v. 6, n. 6, p. 1-9, 2011. |
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https://www.arca.fiocruz.br/handle/icict/40087 |
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1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0021027 |
| dc.identifier.eissn.none.fl_str_mv |
1932-6203 |
| identifier_str_mv |
CARDOSO, Josiane et al. Analysis of Proteasomal Proteolysis during the In Vitro Metacyclogenesis of Trypanosoma cruzi. Plos One, v. 6, n. 6, p. 1-9, 2011. 1932-6203 10.1371/journal.pone.0021027 |
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