Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil

Detalhes bibliográficos
Autor(a) principal: Carvalho, Gabriel Costa de
Data de Publicação: 2012
Outros Autores: Marques, Letícia Helena dos Santos, Gomes, Luciana Inácia, Rabello, Ana Lúcia Teles, Ribeiro, Luiz Cláudio, Scopel, Kezia Katiane Gorza, Tibiriçá, Sandra Helena Cerrato, Coimbra, Elaine Soares, Abramo, Clarice
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7383
Resumo: Universidade Federal de Juiz de Fora. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil
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spelling Carvalho, Gabriel Costa deMarques, Letícia Helena dos SantosGomes, Luciana InáciaRabello, Ana Lúcia TelesRibeiro, Luiz CláudioScopel, Kezia Katiane GorzaTibiriçá, Sandra Helena CerratoCoimbra, Elaine SoaresAbramo, Clarice2014-03-07T14:11:54Z2014-03-07T14:11:54Z2012CARVALHO, Gabriel Costa de et al. Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, n.7, pp. 899-9021678-8060https://www.arca.fiocruz.br/handle/icict/738310.1590/S0074-02762012000700010engFundação Oswaldo CruzPolymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Juiz de Fora. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de Juiz de Fora. Departamento de Estatística. Juiz de Fora, MG, BrasilUniversidade Federal de Juiz de Fora. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, BrasilUniversidade Federal de Juiz de Fora. Faculdade de Medicina. Juiz de Fora, MG, BrasilUniversidade Federal de Juiz de Fora. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, BrasilUniversidade Federal de Juiz de Fora. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, BrasilThis study aimed to evaluate the occurrence of schistosomiasis in areas with low endemicity using polymerase chain reaction (PCR) as a diagnostic method. We analysed faecal samples from 219 individuals residing in Piau and Coronel Pacheco, state of Minas Gerais, Brazil, using a single faecal sample from each individual and two slides of the Kato-Katz technique as a gold standard. Fifteen out of the 219 samples were positive with both methods of diagnosis. One sample was diagnosed as positive by the Kato-Katz technique only and 61 were diagnosed only by PCR. The positivity rates were 7.3% with the Kato-Katz method and 34.7% with PCR. When both techniques were assumed to have 100% specificity and positive individuals were identified by both methods, the sensitivity of the Kato-Katz method was 20.8% and the PCR sensitivity was 98.7%. The Kappa index between the two techniques was 0.234, suggesting weak agreement. The assessment of a single faecal sample by PCR detected more cases of infection than the analysis of one sample with two slides using the Kato-Katz technique, suggesting that PCR can be a useful diagnostic tool, particularly in areas with low endemicity.diagnosisschistosomiasisPCRlow endemicityKato-Katzinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALPolymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil.pdfPolymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil.pdfapplication/pdf328292https://www.arca.fiocruz.br/bitstream/icict/7383/1/Polymerase%20chain%20reaction%20for%20the%20evalution%20of%20Schistosoma%20mansoni%20infection%20in%20two%20low%20endemicity%20areas%20of%20Minas%20Gerais%2c%20Brazil.pdf02329c4699a0a65bd53a114fec5560c1MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7383/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTPolymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil.pdf.txtPolymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil.pdf.txtExtracted texttext/plain22128https://www.arca.fiocruz.br/bitstream/icict/7383/5/Polymerase%20chain%20reaction%20for%20the%20evalution%20of%20Schistosoma%20mansoni%20infection%20in%20two%20low%20endemicity%20areas%20of%20Minas%20Gerais%2c%20Brazil.pdf.txt5dd280356ccc86d758201ccd1006eb4cMD55THUMBNAILPolymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil.pdf.jpgPolymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil.pdf.jpgGenerated Thumbnailimage/jpeg2341https://www.arca.fiocruz.br/bitstream/icict/7383/4/Polymerase%20chain%20reaction%20for%20the%20evalution%20of%20Schistosoma%20mansoni%20infection%20in%20two%20low%20endemicity%20areas%20of%20Minas%20Gerais%2c%20Brazil.pdf.jpg04d70467a10dfdb79551d4b72b2d9e3eMD54icict/73832018-04-06 08:43:57.244oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:43:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil
title Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil
spellingShingle Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil
Carvalho, Gabriel Costa de
diagnosis
schistosomiasis
PCR
low endemicity
Kato-Katz
title_short Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil
title_full Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil
title_fullStr Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil
title_full_unstemmed Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil
title_sort Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil
author Carvalho, Gabriel Costa de
author_facet Carvalho, Gabriel Costa de
Marques, Letícia Helena dos Santos
Gomes, Luciana Inácia
Rabello, Ana Lúcia Teles
Ribeiro, Luiz Cláudio
Scopel, Kezia Katiane Gorza
Tibiriçá, Sandra Helena Cerrato
Coimbra, Elaine Soares
Abramo, Clarice
author_role author
author2 Marques, Letícia Helena dos Santos
Gomes, Luciana Inácia
Rabello, Ana Lúcia Teles
Ribeiro, Luiz Cláudio
Scopel, Kezia Katiane Gorza
Tibiriçá, Sandra Helena Cerrato
Coimbra, Elaine Soares
Abramo, Clarice
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Gabriel Costa de
Marques, Letícia Helena dos Santos
Gomes, Luciana Inácia
Rabello, Ana Lúcia Teles
Ribeiro, Luiz Cláudio
Scopel, Kezia Katiane Gorza
Tibiriçá, Sandra Helena Cerrato
Coimbra, Elaine Soares
Abramo, Clarice
dc.subject.en.pt_BR.fl_str_mv diagnosis
schistosomiasis
PCR
low endemicity
Kato-Katz
topic diagnosis
schistosomiasis
PCR
low endemicity
Kato-Katz
description Universidade Federal de Juiz de Fora. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-03-07T14:11:54Z
dc.date.available.fl_str_mv 2014-03-07T14:11:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Gabriel Costa de et al. Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, n.7, pp. 899-902
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7383
dc.identifier.issn.none.fl_str_mv 1678-8060
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762012000700010
identifier_str_mv CARVALHO, Gabriel Costa de et al. Polymerase chain reaction for the evalution of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, n.7, pp. 899-902
1678-8060
10.1590/S0074-02762012000700010
url https://www.arca.fiocruz.br/handle/icict/7383
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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