Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village

Detalhes bibliográficos
Autor(a) principal: Terashima, Rafael Ponce
Data de Publicação: 2014
Outros Autores: Koskey, Amber M, Reis, Mitermayer Galvão dos, McLellan, Sandra L, Blanton, Ronald Edward
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8702
Resumo: Mercer University School of Medicine. Macon, Georgia, USA / Case Western Reserve University. Center for Global Health and Diseases. Cleveland Ohio, USA
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spelling Terashima, Rafael PonceKoskey, Amber MReis, Mitermayer Galvão dosMcLellan, Sandra LBlanton, Ronald Edward2014-10-31T12:57:23Z2014-10-31T12:57:23Z2014PONCE-TERASHIMA, R. et al. Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village. PLoS Neglected Tropical Diseases, v. 8, n. 10, p. e3186, 2014.1935-2735https://www.arca.fiocruz.br/handle/icict/870210.1371/journal.pntd.0003186engPublic Library of ScienceEsquistossomoseInfecçãoContaminaçãoFezesTransmissãoSaneamentoLimpeza ambientalPrevenção & ControleSources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian villageinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMercer University School of Medicine. Macon, Georgia, USA / Case Western Reserve University. Center for Global Health and Diseases. Cleveland Ohio, USAUniversity of Wisconsin. School of Freshwater Sciences. Great Lakes Water Institute. Milwaukee, Wisconsin, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, BrasilUniversity of Wisconsin. School of Freshwater Sciences. Great Lakes Water Institute. Milwaukee, Wisconsin, USACase Western Reserve University. Center for Global Health and Diseases. Cleveland Ohio, USABACKGROUND: The relationship between poor sanitation and the parasitic infection schistosomiasis is well-known, but still rarely investigated directly and quantitatively. In a Brazilian village we correlated the spatial concentration of human fecal contamination of its main river and the prevalence of schistosomiasis. METHODS: We validated three bacterial markers of contamination in this population by high throughput sequencing of the 16S rRNA gene and qPCR of feces from local residents. The qPCR of genetic markers from the 16S rRNA gene of Bacteroides-Prevotella group, Bacteroides HF8 cluster, and Lachnospiraceae Lachno2 cluster as well as sequencing was performed on georeferenced samples of river water. Ninety-six percent of residents were examined for schistosomiasis. FINDINGS: Sequence of 16S rRNA DNA from stool samples validated the relative human specificity of the HF8 and Lachno 2 fecal indicators compared to animals. The concentration of fecal contamination increased markedly along the river as it passed an increasing proportion of the population on its way downstream as did the sequence reads from bacterial families associated with human feces. Lachnospiraceae provided the most robust signal of human fecal contamination. The prevalence of schistosomiasis likewise increased downstream. Using a linear regression model, a significant correlation was demonstrated between the prevalence of S. mansoni infection and local concentration of human fecal contamination based on the Lachnospiraceae Lachno2 cluster (r2 0.53) as compared to the correlation with the general fecal marker E. coli (r2 0.28). INTERPRETATION: Fecal contamination in rivers has a downstream cumulative effect. The transmission of schistosomiasis correlates with very local factors probably resulting from the distribution of human fecal contamination, the limited movement of snails, and the frequency of water contact near the home. In endemic regions, the combined use of human associated bacterial markers and GIS analysis can quantitatively identify areas with risk for schistosomiasis as well as assess the efficacy of sanitation and environmental interventions for prevention.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8702/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALPonce-Terashima R Sources and....pdfPonce-Terashima R Sources and....pdfapplication/pdf930066https://www.arca.fiocruz.br/bitstream/icict/8702/2/Ponce-Terashima%20R%20Sources%20and....pdf7d7ca12f68b273436285704fc5bd42caMD52TEXTPonce-Terashima R Sources and....pdf.txtPonce-Terashima R Sources and....pdf.txtExtracted texttext/plain50850https://www.arca.fiocruz.br/bitstream/icict/8702/3/Ponce-Terashima%20R%20Sources%20and....pdf.txta3264e221b1656cb18eb66c594bfe1eaMD53icict/87022023-03-15 14:33:23.634oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village
title Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village
spellingShingle Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village
Terashima, Rafael Ponce
Esquistossomose
Infecção
Contaminação
Fezes
Transmissão
Saneamento
Limpeza ambiental
Prevenção & Controle
title_short Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village
title_full Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village
title_fullStr Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village
title_full_unstemmed Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village
title_sort Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village
author Terashima, Rafael Ponce
author_facet Terashima, Rafael Ponce
Koskey, Amber M
Reis, Mitermayer Galvão dos
McLellan, Sandra L
Blanton, Ronald Edward
author_role author
author2 Koskey, Amber M
Reis, Mitermayer Galvão dos
McLellan, Sandra L
Blanton, Ronald Edward
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Terashima, Rafael Ponce
Koskey, Amber M
Reis, Mitermayer Galvão dos
McLellan, Sandra L
Blanton, Ronald Edward
dc.subject.other.pt_BR.fl_str_mv Esquistossomose
Infecção
Contaminação
Fezes
Transmissão
Saneamento
Limpeza ambiental
Prevenção & Controle
topic Esquistossomose
Infecção
Contaminação
Fezes
Transmissão
Saneamento
Limpeza ambiental
Prevenção & Controle
description Mercer University School of Medicine. Macon, Georgia, USA / Case Western Reserve University. Center for Global Health and Diseases. Cleveland Ohio, USA
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-10-31T12:57:23Z
dc.date.available.fl_str_mv 2014-10-31T12:57:23Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PONCE-TERASHIMA, R. et al. Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village. PLoS Neglected Tropical Diseases, v. 8, n. 10, p. e3186, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8702
dc.identifier.issn.none.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0003186
identifier_str_mv PONCE-TERASHIMA, R. et al. Sources and distribution of surface water fecal contamination and prevalence of schistosomiasis in a brazilian village. PLoS Neglected Tropical Diseases, v. 8, n. 10, p. e3186, 2014.
1935-2735
10.1371/journal.pntd.0003186
url https://www.arca.fiocruz.br/handle/icict/8702
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
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