Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni

Detalhes bibliográficos
Autor(a) principal: Waisberg, Michael
Data de Publicação: 2007
Outros Autores: Lobo, Francisco Pereira, Cerqueira, Gustavo M., Passos, Liana Konovaloff Jannotti, Carvalho, Omar dos Santos, El-Sayed, Najib M., Franco, Glória Regina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/6625
Resumo: CAPES, FAPEMIG, NIH,
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spelling Waisberg, MichaelLobo, Francisco PereiraCerqueira, Gustavo M.Passos, Liana Konovaloff JannottiCarvalho, Omar dos SantosEl-Sayed, Najib M.Franco, Glória Regina2013-06-25T18:11:18Z2013-06-25T18:11:18Z2007WAISBERG , Michael et al. Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni. BMC Genomics. 2007 Jun 20;8:181https://www.arca.fiocruz.br/handle/icict/6625CAPES, FAPEMIG, NIH,Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Imunologia e Bioquímica. Laboratório de Genética Bioquímica. Belo Horizonte, MG, Brazil/ The Institute for Genomic Research. Department of Parasite Genomics. Rockville, MD, USAUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Imunologia e Bioquímica. Laboratório de Genética Bioquímica. Belo Horizonte, MG, BrazilThe Institute for Genomic Research. Department of Parasite Genomics, , Rockville, MD, USA/ Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Imunologia e Bioquímica. Laboratório de Genética Molecular de Tripanosomatídeos. Belo Horizonte, MG, BrazilFundação Osvaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Osvaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil,University of Maryland. Department of Cell Biology and Molecular Genetics and Center for Bioinformatics and Computational Biology. College Park, MD, USA / The Institute for Genomic Research. Department of Parasite Genomics, Rockville, MD, USAUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Imunologia e Bioquímica. Laboratório de Genética Bioquímica. Belo Horizonte, MG, BrazilBackground: The parasitic trematode Schistosoma mansoni is one of the major causative agents of Schistosomiasis, a disease that affects approximately 200 million people, mostly in developing countries. Since much of the pathology is associated with eggs laid by the female worm, understanding the mechanisms involved in oogenesis and sexual maturation is an important step towards the discovery of new targets for effective drug therapy. It is known that the adult female worm only develops fully in the presenceof a male worm and that the rates of oviposition and maturation of eggs are significantly increased by mating. In order to study gene transcripts associated with sexual maturation and oviposition, we compared the gene expression profiles of sexually mature and immature parasites using DNA microarrays. Results: For each experiment, three amplified RNA microarray hybridizations and their dye swaps were analyzed. Our results show that 265 transcripts are differentially expressed in adult females and 53 in adult males when mature and immature worms are compared. Of the genes differentially expressed, 55% are expressed at higher levels in paired females while the remaining 45% are more expressed in unpaired ones and 56.6% are expressed at higher levels in paired male worms while the remaining 43.4% are more expressed in immature parasites. Real-time RT-PCR analysis validated the microarray results. Several new maturation associated transcripts were identified. Genes that were up-regulated in single-sex females were mostly related to energy generation (i.e. carbohydrateand protein metabolism, generation of precursor metabolites and energy, cellularcatabolism, and organelle organizationand biogenesis) while genes that were down-regulated related to RNA metabolism, reactive oxygen species metabolism, electron transport, organelle organization and biogenesisand protein biosynthesis. Conclusion: Our results confirm previous observations related togene expression induced by sexual maturation in female schistosome worms. They also increase the list of S. mansoni maturation associated transcripts considerably, therefore opening new and exciting avenues for the study of the conjugal biology and development of new drugs against schistosomes.engMicroarray analysis of gene expression induced by sexual contact in Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSchistosoma mansoniPCRinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL74 MICHAEL WAISBERG M.pdf74 MICHAEL WAISBERG M.pdfapplication/pdf564312https://www.arca.fiocruz.br/bitstream/icict/6625/1/74%20MICHAEL%20WAISBERG%20M.pdf9677dfec3b62b14f775b7f6dee4063cdMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/6625/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXT74 MICHAEL WAISBERG M.pdf.txt74 MICHAEL WAISBERG M.pdf.txtExtracted texttext/plain62443https://www.arca.fiocruz.br/bitstream/icict/6625/5/74%20MICHAEL%20WAISBERG%20M.pdf.txt5d47be14436b8062748deaaa86cf054bMD55THUMBNAIL74 MICHAEL WAISBERG M.pdf.jpg74 MICHAEL WAISBERG M.pdf.jpgGenerated Thumbnailimage/jpeg2417https://www.arca.fiocruz.br/bitstream/icict/6625/4/74%20MICHAEL%20WAISBERG%20M.pdf.jpg49e191def8596be2ecaa71dea1e016afMD54icict/66252018-04-25 15:08:16.317oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-25T18:08:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni
title Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni
spellingShingle Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni
Waisberg, Michael
Schistosoma mansoni
PCR
title_short Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni
title_full Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni
title_fullStr Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni
title_full_unstemmed Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni
title_sort Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni
author Waisberg, Michael
author_facet Waisberg, Michael
Lobo, Francisco Pereira
Cerqueira, Gustavo M.
Passos, Liana Konovaloff Jannotti
Carvalho, Omar dos Santos
El-Sayed, Najib M.
Franco, Glória Regina
author_role author
author2 Lobo, Francisco Pereira
Cerqueira, Gustavo M.
Passos, Liana Konovaloff Jannotti
Carvalho, Omar dos Santos
El-Sayed, Najib M.
Franco, Glória Regina
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Waisberg, Michael
Lobo, Francisco Pereira
Cerqueira, Gustavo M.
Passos, Liana Konovaloff Jannotti
Carvalho, Omar dos Santos
El-Sayed, Najib M.
Franco, Glória Regina
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
PCR
topic Schistosoma mansoni
PCR
description CAPES, FAPEMIG, NIH,
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2013-06-25T18:11:18Z
dc.date.available.fl_str_mv 2013-06-25T18:11:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv WAISBERG , Michael et al. Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni. BMC Genomics. 2007 Jun 20;8:181
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/6625
identifier_str_mv WAISBERG , Michael et al. Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni. BMC Genomics. 2007 Jun 20;8:181
url https://www.arca.fiocruz.br/handle/icict/6625
dc.language.iso.fl_str_mv eng
language eng
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