Kinome siRNA screen identifies novel cell-type specific dengue host target genes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16053 |
Resumo: | Institut Pasteur Korea. Discovery Biology Group. Seongnam-si, South Korea. |
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Kwon, Yong-JunHeo, JinyeongWong, Hazel E.E.Cruz, Deu John M.Velumani, SumathySilva, Camila T. daMosimann, Ana Luiza PamplonaSantos, Claudia Nunes Duarte dosFreitas-Junior, Lucio HolandaFink, Katja2016-10-03T18:30:17Z2016-10-03T18:30:17Z2014Kwon, Yong-Jun et al. Kinome siRNA screen identifies novel cell-type specific dengue host target genes. Antiviral Research, n. 110, p. 20–30, 2014.0166-3542https://www.arca.fiocruz.br/handle/icict/1605310.1016/j.antiviral.2014.07.006engMike BrayKinome siRNA screen identifies novel cell-type specific dengue host target genesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstitut Pasteur Korea. Discovery Biology Group. Seongnam-si, South Korea.Institut Pasteur Korea. Discovery Biology Group. Seongnam-si, South Korea.Singapore Immunology Network. Agency for Science, Technology and Research. Buona Vista, Singapore.Institut Pasteur Korea. Center for Neglected Diseases Drug Discovery. Seongnam-si, South Korea.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Singapore Immunology Network. Agency for Science, Technology and Research. Buona Vista, Singapore.Institut Pasteur Korea. Center for Neglected Diseases Drug Discovery. Seongnam-si, South Korea.Institut Pasteur Korea. Center for Neglected Diseases Drug Discovery. Seongnam-si, South Korea.Singapore Immunology Network. Agency for Science, Technology and Research. Buona Vista, Singapore.Dengue is a global emerging infectious disease, with no specific treatment available. To identify novel human host cell targets important for dengue virus infection and replication, an image-based high-throughput siRNA assay screening of a human kinome siRNA library was conducted using human hepatocyte cell line Huh7 infected with a recent dengue serotype 2 virus isolate BR DEN2 01-01. In the primary siRNA screening of 779 kinase-related genes, knockdown of 22 genes showed a reduction in DENV-2 infection. Conversely, knockdown of 8 genes enhanced viral infection. To assess host cell specificity, the confirmed hits were tested in the DENV-infected monocytic cell line U937. While the expression of EIF2AK3, ETNK2 and SMAD7 was regulated in both cell lines after infection, most kinases were hepatocyte-specific. Monocytic cells represent initial targets of infection and an antiviral treatment targeting these cells is probably most effective to reduce initial viral load. In turn, infection of the liver could contribute to pathogenesis, and the novel hepatocyte-specific human targets identified here could be important for dengue infection and pathogenesis.siRNAKinaseDengueHost cell targetsViral TropismRNA Interferente PequenoTropismo Viralinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/16053/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALKinome siRNA screen.pdfKinome siRNA screen.pdfapplication/pdf4048808https://www.arca.fiocruz.br/bitstream/icict/16053/2/Kinome%20siRNA%20screen.pdfbf050acf9f684cf1d04218d8e7424b31MD52TEXTKinome siRNA screen.pdf.txtKinome siRNA screen.pdf.txtExtracted texttext/plain44149https://www.arca.fiocruz.br/bitstream/icict/16053/3/Kinome%20siRNA%20screen.pdf.txtd4ed77c16650976a996518e46dcb574eMD53icict/160532019-11-01 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| dc.title.pt_BR.fl_str_mv |
Kinome siRNA screen identifies novel cell-type specific dengue host target genes |
| title |
Kinome siRNA screen identifies novel cell-type specific dengue host target genes |
| spellingShingle |
Kinome siRNA screen identifies novel cell-type specific dengue host target genes Kwon, Yong-Jun siRNA Kinase Dengue Host cell targets Viral Tropism RNA Interferente Pequeno Tropismo Viral |
| title_short |
Kinome siRNA screen identifies novel cell-type specific dengue host target genes |
| title_full |
Kinome siRNA screen identifies novel cell-type specific dengue host target genes |
| title_fullStr |
Kinome siRNA screen identifies novel cell-type specific dengue host target genes |
| title_full_unstemmed |
Kinome siRNA screen identifies novel cell-type specific dengue host target genes |
| title_sort |
Kinome siRNA screen identifies novel cell-type specific dengue host target genes |
| author |
Kwon, Yong-Jun |
| author_facet |
Kwon, Yong-Jun Heo, Jinyeong Wong, Hazel E.E. Cruz, Deu John M. Velumani, Sumathy Silva, Camila T. da Mosimann, Ana Luiza Pamplona Santos, Claudia Nunes Duarte dos Freitas-Junior, Lucio Holanda Fink, Katja |
| author_role |
author |
| author2 |
Heo, Jinyeong Wong, Hazel E.E. Cruz, Deu John M. Velumani, Sumathy Silva, Camila T. da Mosimann, Ana Luiza Pamplona Santos, Claudia Nunes Duarte dos Freitas-Junior, Lucio Holanda Fink, Katja |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Kwon, Yong-Jun Heo, Jinyeong Wong, Hazel E.E. Cruz, Deu John M. Velumani, Sumathy Silva, Camila T. da Mosimann, Ana Luiza Pamplona Santos, Claudia Nunes Duarte dos Freitas-Junior, Lucio Holanda Fink, Katja |
| dc.subject.en.pt_BR.fl_str_mv |
siRNA Kinase Dengue Host cell targets |
| topic |
siRNA Kinase Dengue Host cell targets Viral Tropism RNA Interferente Pequeno Tropismo Viral |
| dc.subject.en.En.fl_str_mv |
Viral Tropism |
| dc.subject.decs.pt_BR.fl_str_mv |
RNA Interferente Pequeno Tropismo Viral |
| description |
Institut Pasteur Korea. Discovery Biology Group. Seongnam-si, South Korea. |
| publishDate |
2014 |
| dc.date.issued.fl_str_mv |
2014 |
| dc.date.accessioned.fl_str_mv |
2016-10-03T18:30:17Z |
| dc.date.available.fl_str_mv |
2016-10-03T18:30:17Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
Kwon, Yong-Jun et al. Kinome siRNA screen identifies novel cell-type specific dengue host target genes. Antiviral Research, n. 110, p. 20–30, 2014. |
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https://www.arca.fiocruz.br/handle/icict/16053 |
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0166-3542 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1016/j.antiviral.2014.07.006 |
| identifier_str_mv |
Kwon, Yong-Jun et al. Kinome siRNA screen identifies novel cell-type specific dengue host target genes. Antiviral Research, n. 110, p. 20–30, 2014. 0166-3542 10.1016/j.antiviral.2014.07.006 |
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https://www.arca.fiocruz.br/handle/icict/16053 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Mike Bray |
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Mike Bray |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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