IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process

Detalhes bibliográficos
Autor(a) principal: Du-Rocher, Bárbara
Data de Publicação: 2020
Outros Autores: Binato, Renata, Freitas Junior, Julio Cesar Madureira de, Corrêa, Stephany, Mencalha, André Luiz, Morgado-Díaz, José Andrés, Abdelhay, Eliana Saul Furquim Werneck
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45551
Resumo: Instituto Nacional de Câncer. Centro de Transplante de Medula óssea. Laboratório de Células-tronco. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.
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spelling Du-Rocher, BárbaraBinato, RenataFreitas Junior, Julio Cesar Madureira deCorrêa, StephanyMencalha, André LuizMorgado-Díaz, José AndrésAbdelhay, Eliana Saul Furquim Werneck2021-01-10T18:23:40Z2021-01-10T18:23:40Z2020DU-ROCHER, Bárbara et al. IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process. Stem Cell Reviews and Reports, v. 16,p. 1266-1279, Oct. 2020.1550-8943https://www.arca.fiocruz.br/handle/icict/4555110.1007/s12015-020-10051-4engSpringerIFNyCélulas estromais mesenquimais (MSCs)Matriz de chipsIL-17ImunomodulaçãoMesenchymal stromal cells (MSCs)IFNyIL-17Chip arrayImmunomodulationIL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Processinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Câncer. Centro de Transplante de Medula óssea. Laboratório de Células-tronco. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Centro de Transplante de Medula óssea. Laboratório de Células-tronco. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Programa de Oncobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Centro de Transplante de Medula óssea. Laboratório de Células-tronco. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biofísica e Biometria. Laboratório de Biologia do Câncer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Programa de Oncobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Centro de Transplante de Medula óssea. Laboratório de Células-tronco. Rio de Janeiro, RJ, Brasil.Mesenchymal stromal cells (MSCs) were first used as a source for cell therapy in 1995; however, despite their versatility and unambiguous demonstration of efficacy and safety in preclinical/phase I studies, the positive effect of MSCs in human phase III studies did not resemble the success obtained in mouse models of disease. This dissonance highlights the need to more thoroughly study the immunobiology of MSCs to make better use of these cells. Thus, we aimed to study the immunobiology of MSCs by using chip array analysis as a method for general screening to obtain a global picture in our model study and found IFNy and IL-17 signaling as the first two “top canonical pathways” involved in MSCs immunomodulation. The role of IFNy in triggering the immunosuppressive properties of MSCs is well recognized by many groups; however, the role of IL-17 in this process remains uncertain. Interestingly, in contrast to IFNy, which actively improved the MSCs-mediated immunosuppression, IL-17 did not improve directly the MSCs-mediated immunosuppression. Instead, IL-17 signaling induced the migration ofMSCs and inflammatory cells, bringing these cell types together and increasing the likelihood of the lymphocytes sensing the immunosuppressive molecules produced by the MSCs. These effects also correlated with high levels of cytokine/chemokine production and metalloprotease activation by MSCs. Importantly, this treatment maintained the MSCs safety profile by not inducing the expression of molecules related to antigen presentation. In this way, our findings highlight the possibility of using IL-17, in combination with IFNy, to prime MSCs for cell therapy to improve their biological properties and thus their therapeutic efficacy. Finally, the use of preactivated MSCs may also minimize variations among MSCs to produce more uniformtherapeutic products. In the not-so-distant future, we envisage a portfolio of MSCs activated by different cocktails specifically designed to target and treat specific diseases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45551/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRenataBinato_ElianaAbdelhay_etal_IOC_2020.pdfRenataBinato_ElianaAbdelhay_etal_IOC_2020.pdfapplication/pdf2558273https://www.arca.fiocruz.br/bitstream/icict/45551/2/RenataBinato_ElianaAbdelhay_etal_IOC_2020.pdfe4c9986a21effd646e4b92cba9b263eeMD52TEXTRenataBinato_ElianaAbdelhay_etal_IOC_2020.pdf.txtRenataBinato_ElianaAbdelhay_etal_IOC_2020.pdf.txtExtracted texttext/plain55823https://www.arca.fiocruz.br/bitstream/icict/45551/3/RenataBinato_ElianaAbdelhay_etal_IOC_2020.pdf.txtbf630ea8694f53e4213175f20dbb5e2dMD53icict/455512022-03-24 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dc.title.pt_BR.fl_str_mv IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process
title IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process
spellingShingle IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process
Du-Rocher, Bárbara
IFNy
Células estromais mesenquimais (MSCs)
Matriz de chips
IL-17
Imunomodulação
Mesenchymal stromal cells (MSCs)
IFNy
IL-17
Chip array
Immunomodulation
title_short IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process
title_full IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process
title_fullStr IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process
title_full_unstemmed IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process
title_sort IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process
author Du-Rocher, Bárbara
author_facet Du-Rocher, Bárbara
Binato, Renata
Freitas Junior, Julio Cesar Madureira de
Corrêa, Stephany
Mencalha, André Luiz
Morgado-Díaz, José Andrés
Abdelhay, Eliana Saul Furquim Werneck
author_role author
author2 Binato, Renata
Freitas Junior, Julio Cesar Madureira de
Corrêa, Stephany
Mencalha, André Luiz
Morgado-Díaz, José Andrés
Abdelhay, Eliana Saul Furquim Werneck
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Du-Rocher, Bárbara
Binato, Renata
Freitas Junior, Julio Cesar Madureira de
Corrêa, Stephany
Mencalha, André Luiz
Morgado-Díaz, José Andrés
Abdelhay, Eliana Saul Furquim Werneck
dc.subject.other.pt_BR.fl_str_mv IFNy
Células estromais mesenquimais (MSCs)
Matriz de chips
IL-17
Imunomodulação
topic IFNy
Células estromais mesenquimais (MSCs)
Matriz de chips
IL-17
Imunomodulação
Mesenchymal stromal cells (MSCs)
IFNy
IL-17
Chip array
Immunomodulation
dc.subject.en.pt_BR.fl_str_mv Mesenchymal stromal cells (MSCs)
IFNy
IL-17
Chip array
Immunomodulation
description Instituto Nacional de Câncer. Centro de Transplante de Medula óssea. Laboratório de Células-tronco. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-01-10T18:23:40Z
dc.date.available.fl_str_mv 2021-01-10T18:23:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DU-ROCHER, Bárbara et al. IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process. Stem Cell Reviews and Reports, v. 16,p. 1266-1279, Oct. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45551
dc.identifier.issn.pt_BR.fl_str_mv 1550-8943
dc.identifier.doi.none.fl_str_mv 10.1007/s12015-020-10051-4
identifier_str_mv DU-ROCHER, Bárbara et al. IL-17 Triggers Invasive and Migratory Properties in Human MSCs, while IFNy Favors their Immunosuppressive Capabilities: Implications for the “Licensing” Process. Stem Cell Reviews and Reports, v. 16,p. 1266-1279, Oct. 2020.
1550-8943
10.1007/s12015-020-10051-4
url https://www.arca.fiocruz.br/handle/icict/45551
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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